Chlorine in PDB 1sez: Crystal Structure of Protoporphyrinogen IX Oxidase

All present enzymatic activity of Crystal Structure of Protoporphyrinogen IX Oxidase:
1.3.3.4;

The structure of Crystal Structure of Protoporphyrinogen IX Oxidase, PDB code: 1sez was solved by M.Koch, C.Breithaupt, R.Kiefersauer, J.Freigang, R.Huber, A.Messerschmidt, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.73 / 2.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	119.090, 147.250, 127.040, 90.00, 90.00, 90.00
R / Rfree (%)	22.7 / 29.3

The structure of Crystal Structure of Protoporphyrinogen IX Oxidase also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Bromine	(Br)	2 atoms

The binding sites of Chlorine atom in the Crystal Structure of Protoporphyrinogen IX Oxidase (pdb code 1sez). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Protoporphyrinogen IX Oxidase, PDB code: 1sez:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Protoporphyrinogen IX Oxidase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Protoporphyrinogen IX Oxidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl601 b:70.5 occ:1.00 CL1 A:OMN601 0.0 70.5 1.0 C10 A:OMN601 1.7 61.6 1.0 C11 A:OMN601 2.7 59.9 1.0 C8 A:OMN601 2.8 59.2 1.0 O2 A:OMN601 3.1 57.2 1.0 C9 A:OMN601 3.2 57.0 1.0 CA A:GLY354 3.4 48.4 1.0 N A:GLY354 3.4 48.0 1.0 C A:GLY354 3.5 49.3 1.0 N A:VAL355 3.8 49.4 1.0 C A:PHE353 3.9 49.0 1.0 O A:GLY354 4.0 48.9 1.0 C12 A:OMN601 4.0 59.0 1.0 C7 A:OMN601 4.1 57.3 1.0 CG A:ARG98 4.1 61.3 1.0 O A:PHE353 4.2 49.5 1.0 O1 A:OMN601 4.2 56.1 1.0 CB A:PHE353 4.2 49.1 1.0 CB A:ARG98 4.3 58.7 1.0 CD2 A:LEU372 4.3 44.6 1.0 C6 A:OMN601 4.6 58.1 1.0 CA A:VAL355 4.6 48.6 1.0 CD A:ARG98 4.6 65.5 1.0 CG A:LEU356 4.6 47.5 1.0 CA A:PHE353 4.7 49.9 1.0 NE A:ARG98 4.7 69.8 1.0 CA A:ARG98 4.7 57.0 1.0 C A:VAL355 4.7 49.0 1.0 CD2 A:LEU356 4.8 47.8 1.0 O A:THR371 4.9 44.2 1.0 O A:VAL355 5.0 50.8 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Protoporphyrinogen IX Oxidase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Protoporphyrinogen IX Oxidase within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl604 b:73.4 occ:1.00 CL1 B:OMN604 0.0 73.4 1.0 C10 B:OMN604 1.7 66.9 1.0 C11 B:OMN604 2.7 66.3 1.0 C8 B:OMN604 2.8 66.2 1.0 C9 B:OMN604 3.2 65.6 1.0 CA B:GLY354 3.4 49.7 1.0 C B:GLY354 3.5 50.2 1.0 O2 B:OMN604 3.6 68.2 1.0 N B:GLY354 3.6 48.6 1.0 N B:VAL355 3.7 50.2 1.0 O1 B:OMN604 3.9 65.5 1.0 C12 B:OMN604 4.0 64.1 1.0 O B:GLY354 4.0 51.1 1.0 C7 B:OMN604 4.1 64.8 1.0 CG B:ARG98 4.1 61.8 1.0 C B:PHE353 4.2 50.6 1.0 CG B:LEU356 4.3 48.8 1.0 CB B:ARG98 4.3 58.9 1.0 CD2 B:LEU372 4.4 45.9 1.0 C B:VAL355 4.4 52.2 1.0 CA B:VAL355 4.4 51.5 1.0 CD B:ARG98 4.5 68.5 1.0 CD2 B:LEU356 4.5 51.0 1.0 O B:PHE353 4.5 48.9 1.0 O B:VAL355 4.5 52.3 1.0 CB B:PHE353 4.5 51.3 1.0 C6 B:OMN604 4.6 63.7 1.0 NE B:ARG98 4.7 73.2 1.0 CA B:ARG98 4.8 58.6 1.0 CD1 B:LEU356 4.8 52.5 1.0 O B:THR371 4.9 47.7 1.0 N B:LEU356 4.9 50.9 1.0 F4 B:OMN604 5.0 64.2 1.0 CA B:PHE353 5.0 52.5 1.0

M.Koch, C.Breithaupt, R.Kiefersauer, J.Freigang, R.Huber, A.Messerschmidt. Crystal Structure of Protoporphyrinogen IX Oxidase: A Key Enzyme in Haem and Chlorophyll Biosynthesis. Embo J. V. 23 1720 2004.
ISSN: ISSN 0261-4189
PubMed: 15057273
DOI: 10.1038/SJ.EMBOJ.7600189