Chlorine in PDB 1t8f: Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A

All present enzymatic activity of Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A:
3.2.1.17;

The structure of Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A, PDB code: 1t8f was solved by M.M.He, Z.A.Wood, W.A.Baase, H.Xiao, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.45 / 2.15
Space group	P 62 2 2
Cell size a, b, c (Å), α, β, γ (°)	120.518, 120.518, 50.317, 90.00, 90.00, 120.00
R / Rfree (%)	22.4 / 27.5

The binding sites of Chlorine atom in the Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A (pdb code 1t8f). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A, PDB code: 1t8f:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl800 b:17.6 occ:1.00 N A:ASN144 3.3 19.9 1.0 O A:HOH945 3.3 22.4 1.0 N A:ARG145 3.4 20.5 1.0 CB A:ASN144 3.5 21.6 1.0 ND2 A:ASN144 3.5 18.5 1.0 C A:THR142 3.5 20.7 1.0 CA A:THR142 3.5 19.8 1.0 N A:PRO143 3.7 18.9 1.0 CA A:ASN144 3.8 19.9 1.0 CB A:THR142 3.8 18.8 1.0 CD A:PRO143 3.9 22.1 1.0 O A:THR142 4.0 23.1 1.0 CG A:ASN144 4.0 22.2 1.0 C A:ASN144 4.1 19.2 1.0 C A:PRO143 4.3 19.0 1.0 CB A:ARG145 4.3 18.5 1.0 CG2 A:THR142 4.3 18.3 1.0 CA A:ARG145 4.5 21.0 1.0 CG A:PRO143 4.5 21.7 1.0 CA A:PRO143 4.5 18.4 1.0 O A:HOH921 4.7 25.9 1.0 N A:THR142 4.9 20.0 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Phage T4 Lysozyme Mutant R14A/K16A/I17A/K19A/T21A/E22A/C54T/C97A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl900 b:18.8 occ:0.50 O A:GLU11 3.9 20.7 1.0 CA A:GLY12 4.4 22.9 1.0 N A:LEU13 4.5 24.8 1.0 O A:HOH928 4.5 21.1 1.0 OH A:TYR18 4.6 24.9 1.0 C A:GLY12 4.6 22.6 1.0 O A:LEU13 4.6 21.7 1.0 CZ A:TYR18 4.7 26.5 1.0 CB A:ALA14 4.7 24.2 1.0 C A:LEU13 4.8 23.3 1.0 CE2 A:TYR18 4.9 28.6 1.0 C A:GLU11 4.9 19.6 1.0

M.M.He, Z.A.Wood, W.A.Baase, H.Xiao, B.W.Matthews. Alanine-Scanning Mutagenesis of the Beta-Sheet Region of Phage T4 Lysozyme Suggests That Tertiary Context Has A Dominant Effect on Beta-Sheet Formation. Protein Sci. V. 13 2716 2004.
ISSN: ISSN 0961-8368
PubMed: 15340171
DOI: 10.1110/PS.04875504