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Chlorine in PDB 1uxj: Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface

Enzymatic activity of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface

All present enzymatic activity of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface:
1.1.1.37;

Protein crystallography data

The structure of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface, PDB code: 1uxj was solved by A.Bjork, B.Dalhus, D.Mantzilas, V.G.H.Eijsink, R.Sirevag, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 1.75
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 105.247, 105.247, 102.196, 90.00, 90.00, 120.00
R / Rfree (%) 17.7 / 21.1

Other elements in 1uxj:

The structure of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface also contains other interesting chemical elements:

Cadmium (Cd) 9 atoms
Sodium (Na) 3 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface (pdb code 1uxj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface, PDB code: 1uxj:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 1uxj

Go back to Chlorine Binding Sites List in 1uxj
Chlorine binding site 1 out of 2 in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1313

b:24.8
occ:1.00
CD A:CD1311 2.5 26.8 0.8
O A:HOH2312 3.4 22.6 1.0
N A:ALA124 3.4 20.2 1.0
CG2 A:THR302 3.7 27.7 1.0
CB A:ASP123 3.7 21.0 1.0
CB A:PRO121 3.8 23.8 1.0
CB A:ALA124 3.8 20.4 1.0
CB A:THR302 3.9 29.4 1.0
N A:ASP123 3.9 19.4 1.0
O A:HOH2154 4.0 14.2 1.0
CA A:ASP123 4.1 21.0 1.0
OG1 A:THR302 4.2 29.6 1.0
O A:HOH2195 4.2 24.6 1.0
C A:ASP123 4.2 21.2 1.0
CA A:ALA124 4.2 20.6 1.0
OE2 A:GLU178 4.2 34.1 1.0
OE1 A:GLU178 4.3 36.0 1.0
C A:PRO121 4.3 22.4 1.0
O A:PRO121 4.4 20.7 1.0
CG1 A:VAL299 4.5 25.0 1.0
CA A:PRO121 4.5 22.2 1.0
CG A:ASP123 4.6 23.8 1.0
OD2 A:ASP123 4.6 20.4 1.0
N A:LEU122 4.7 21.4 1.0
CD A:GLU178 4.7 31.8 1.0
CG A:PRO121 4.7 23.1 1.0
C A:LEU122 4.8 19.8 1.0
CA A:VAL299 4.8 25.5 1.0
O A:VAL299 4.8 25.2 1.0

Chlorine binding site 2 out of 2 in 1uxj

Go back to Chlorine Binding Sites List in 1uxj
Chlorine binding site 2 out of 2 in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl1314

b:25.6
occ:1.00
CD C:CD1311 2.5 25.4 0.9
N C:ALA124 3.4 21.1 1.0
O C:HOH2300 3.4 23.9 1.0
CB C:ASP123 3.6 19.3 1.0
CG2 C:THR302 3.7 27.6 1.0
CB C:PRO121 3.7 22.8 1.0
CB C:ALA124 3.8 22.4 1.0
N C:ASP123 3.9 20.1 1.0
CB C:THR302 3.9 28.3 1.0
O C:HOH2137 3.9 8.3 1.0
O C:HOH2181 4.0 13.9 1.0
OE2 C:GLU178 4.0 36.1 1.0
CA C:ASP123 4.1 19.9 1.0
OE1 C:GLU178 4.2 34.5 1.0
CA C:ALA124 4.2 22.4 1.0
C C:ASP123 4.2 21.0 1.0
OG1 C:THR302 4.3 28.2 1.0
C C:PRO121 4.3 21.5 1.0
O C:PRO121 4.3 19.9 1.0
CA C:PRO121 4.5 22.4 1.0
CD C:GLU178 4.5 32.5 1.0
CG1 C:VAL299 4.5 23.7 1.0
CG C:ASP123 4.6 21.9 1.0
OD2 C:ASP123 4.6 21.4 1.0
CG C:PRO121 4.6 22.2 1.0
N C:LEU122 4.7 21.4 1.0
C C:LEU122 4.8 18.7 1.0
O C:VAL299 4.8 27.5 1.0
CA C:VAL299 4.9 25.3 1.0

Reference:

A.Bjork, B.Dalhus, D.Mantzilas, R.Sirevag, V.G.H.Eijsink. Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface. J.Mol.Biol. V. 341 1215 2004.
ISSN: ISSN 0022-2836
PubMed: 15321717
DOI: 10.1016/J.JMB.2004.06.079
Page generated: Sat Dec 12 08:51:13 2020

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