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Chlorine in PDB 1uxk: Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface

Enzymatic activity of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface

All present enzymatic activity of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface:
1.1.1.37;

Protein crystallography data

The structure of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface, PDB code: 1uxk was solved by A.Bjork, B.Dalhus, D.Mantzilas, V.G.H.Eijsink, R.Sirevag, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 1.80
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 105.405, 105.405, 102.305, 90.00, 90.00, 120.00
R / Rfree (%) 17.8 / 21.4

Other elements in 1uxk:

The structure of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface also contains other interesting chemical elements:

Cadmium (Cd) 10 atoms
Sodium (Na) 6 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface (pdb code 1uxk). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface, PDB code: 1uxk:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 1uxk

Go back to Chlorine Binding Sites List in 1uxk
Chlorine binding site 1 out of 2 in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl1313

b:26.2
occ:1.00
CD A:CD1311 2.5 28.5 0.9
O A:HOH2308 3.3 28.7 1.0
N A:ALA124 3.4 22.1 1.0
CG2 A:THR302 3.6 30.1 1.0
CB A:ASP123 3.7 22.6 1.0
CB A:PRO121 3.7 23.1 1.0
CB A:ALA124 3.8 23.3 1.0
CB A:THR302 3.9 32.2 1.0
N A:ASP123 4.0 20.9 1.0
O A:HOH2142 4.0 12.4 1.0
O A:HOH2077 4.1 16.8 1.0
OE1 A:GLU178 4.1 32.7 1.0
CA A:ASP123 4.1 22.0 1.0
OE2 A:GLU178 4.2 33.8 1.0
C A:ASP123 4.2 21.7 1.0
OG1 A:THR302 4.2 31.8 1.0
CA A:ALA124 4.2 21.1 1.0
O A:PRO121 4.3 21.7 1.0
C A:PRO121 4.3 22.7 1.0
CG1 A:VAL299 4.5 23.0 1.0
CA A:PRO121 4.5 22.8 1.0
CG A:PRO121 4.6 24.5 1.0
CG A:ASP123 4.6 23.6 1.0
CD A:GLU178 4.6 31.2 1.0
OD2 A:ASP123 4.7 23.6 1.0
N A:LEU122 4.7 22.7 1.0
CA A:VAL299 4.8 25.1 1.0
O A:VAL299 4.8 23.5 1.0
C A:LEU122 4.8 21.2 1.0
CG2 A:VAL299 5.0 23.5 1.0

Chlorine binding site 2 out of 2 in 1uxk

Go back to Chlorine Binding Sites List in 1uxk
Chlorine binding site 2 out of 2 in the Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl1312

b:25.0
occ:1.00
CD A:CD1309 2.5 20.1 1.0
O A:HOH2204 2.9 31.9 1.0
N C:GLU281 3.3 15.8 1.0
O A:HOH2206 3.5 19.6 1.0
O C:HOH2286 3.6 34.5 1.0
OE2 A:GLU195 3.7 26.8 1.0
O C:GLU281 3.7 16.0 1.0
O C:HOH2291 3.8 22.2 1.0
CA C:LEU280 3.9 16.2 1.0
CB C:LEU280 4.0 14.7 1.0
O C:HOH2292 4.0 19.1 1.0
C C:LEU280 4.0 16.5 1.0
C C:GLU281 4.1 17.3 1.0
CD2 C:LEU280 4.1 16.4 1.0
CA C:GLU281 4.2 15.5 1.0
CG A:GLU195 4.3 26.2 1.0
CD A:GLU195 4.5 28.7 1.0
O A:GLU195 4.5 21.9 1.0
CB C:GLU281 4.6 17.7 1.0
CG C:LEU280 4.7 15.9 1.0
N C:LEU282 4.9 17.2 1.0

Reference:

A.Bjork, B.Dalhus, D.Mantzilas, R.Sirevag, V.G.H.Eijsink. Large Improvement in the Thermal Stability of A Tetrameric Malate Dehydrogenase By Single Point Mutations at the Dimer-Dimer Interface. J.Mol.Biol. V. 341 1215 2004.
ISSN: ISSN 0022-2836
PubMed: 15321717
DOI: 10.1016/J.JMB.2004.06.079
Page generated: Sat Jul 20 02:52:14 2024

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