Chlorine in PDB 1wl6: Mg-Substituted Form of E. Coli Aminopeptidase P

All present enzymatic activity of Mg-Substituted Form of E. Coli Aminopeptidase P:
3.4.11.9;

The structure of Mg-Substituted Form of E. Coli Aminopeptidase P, PDB code: 1wl6 was solved by S.C.Graham, C.S.Bond, H.C.Freeman, J.M.Guss, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.26 / 2.00
Space group	P 64 2 2
Cell size a, b, c (Å), α, β, γ (°)	177.733, 177.733, 96.484, 90.00, 90.00, 120.00
R / Rfree (%)	14.8 / 17.3

The structure of Mg-Substituted Form of E. Coli Aminopeptidase P also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Chlorine atom in the Mg-Substituted Form of E. Coli Aminopeptidase P (pdb code 1wl6). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Mg-Substituted Form of E. Coli Aminopeptidase P, PDB code: 1wl6:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Mg-Substituted Form of E. Coli Aminopeptidase P


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Mg-Substituted Form of E. Coli Aminopeptidase P within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl803 b:26.1 occ:1.00 O A:HOH1210 3.1 38.2 1.0 O A:HOH1224 3.1 41.1 1.0 O A:HOH1346 3.3 43.1 1.0 N A:VAL80 3.3 20.9 1.0 N A:SER111 3.5 19.9 1.0 CA A:ARG79 3.7 18.0 1.0 CB A:SER111 3.8 21.2 1.0 N A:PHE110 3.8 18.2 1.0 CG1 A:VAL80 3.8 23.2 1.0 OG A:SER111 3.9 20.8 1.0 CB A:VAL80 4.0 21.3 1.0 C A:ARG79 4.0 19.1 1.0 O A:ASN78 4.1 15.3 1.0 CB A:PHE110 4.2 18.2 1.0 CA A:SER111 4.2 21.4 1.0 CB A:ALA109 4.3 19.7 1.0 CA A:VAL80 4.3 21.7 1.0 CA A:PHE110 4.3 17.5 1.0 C A:PHE110 4.4 19.2 1.0 CB A:ARG79 4.4 17.4 1.0 C A:ALA109 4.5 19.0 1.0 N A:ARG79 4.6 17.2 1.0 CA A:ALA109 4.6 18.6 1.0 CG A:ARG79 4.7 19.6 1.0 C A:ASN78 4.7 17.4 1.0

Chlorine binding site 2 out of 2 in the Mg-Substituted Form of E. Coli Aminopeptidase P


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Mg-Substituted Form of E. Coli Aminopeptidase P within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl804 b:30.7 occ:1.00 N A:GLY351 3.1 15.1 1.0 O A:HOH983 3.2 25.3 1.0 NH2 A:ARG370 3.4 25.3 1.0 C11 A:XPE901 3.7 40.2 1.0 CB A:HIS350 3.9 17.2 1.0 CA A:GLY351 3.9 15.8 1.0 CA A:HIS350 4.0 16.4 1.0 C A:HIS350 4.1 15.5 1.0 CZ A:ARG370 4.4 25.0 1.0 O A:HOH1164 4.4 35.3 1.0 C12 A:XPE901 4.4 37.7 1.0 NH1 A:ARG370 4.5 25.9 1.0 O13 A:XPE901 4.5 37.0 1.0 O10 A:XPE901 4.5 41.1 1.0 C1 A:IPA902 4.6 28.3 1.0 C9 A:XPE901 4.6 42.8 1.0 C A:GLY351 4.9 15.5 1.0 O A:GLY351 4.9 13.5 1.0

S.C.Graham, C.S.Bond, H.C.Freeman, J.M.Guss. Structural and Functional Implications of Metal Ion Selection in Aminopeptidase P, A Metalloprotease with A Dinuclear Metal Center Biochemistry V. 44 13820 2005.
ISSN: ISSN 0006-2960
PubMed: 16229471
DOI: 10.1021/BI0512849