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Chlorine in PDB 1wx5: Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal

Enzymatic activity of Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal

All present enzymatic activity of Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal:
1.14.18.1;

Protein crystallography data

The structure of Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal, PDB code: 1wx5 was solved by Y.Matoba, T.Kumagai, A.Yamamoto, H.Yoshitsu, M.Sugiyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 2.02
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 58.850, 93.200, 65.590, 90.00, 93.80, 90.00
R / Rfree (%) 20.1 / 29.1

Other elements in 1wx5:

The structure of Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal also contains other interesting chemical elements:

Sodium (Na) 3 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal (pdb code 1wx5). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal, PDB code: 1wx5:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 1wx5

Go back to Chlorine Binding Sites List in 1wx5
Chlorine binding site 1 out of 2 in the Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl283

b:19.1
occ:1.00
NE2 A:HIS265 3.2 12.7 1.0
N A:PHE271 3.5 13.2 1.0
O A:HOH459 3.5 34.9 1.0
CB A:THR270 3.6 12.7 1.0
CA A:THR270 3.7 12.2 1.0
CD1 A:PHE271 3.8 11.2 1.0
CD1 A:LEU274 4.0 26.4 1.0
CD2 A:HIS265 4.0 5.5 1.0
CG2 A:THR270 4.0 19.5 1.0
C A:THR270 4.1 13.1 1.0
CE1 A:HIS265 4.2 11.9 1.0
CG2 A:THR266 4.3 6.0 1.0
CA A:PHE271 4.6 11.2 1.0
CB A:PHE271 4.6 10.7 1.0
CE1 A:PHE271 4.6 16.4 1.0
O A:PHE271 4.7 10.8 1.0
CG A:PHE271 4.7 10.1 1.0
NH2 A:ARG228 4.7 55.3 1.0
CG A:LEU274 4.8 28.4 1.0
OG1 A:THR270 4.9 15.7 1.0

Chlorine binding site 2 out of 2 in 1wx5

Go back to Chlorine Binding Sites List in 1wx5
Chlorine binding site 2 out of 2 in the Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Copper-Free Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein in the Monoclinic Crystal within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl284

b:29.5
occ:1.00
NE2 A:GLN227 3.1 21.8 1.0
NE1 A:TRP223 3.4 5.2 1.0
N A:TYR235 3.7 13.3 1.0
CG A:GLN227 3.7 16.8 1.0
CZ2 A:TRP223 3.7 9.9 1.0
CD2 A:LEU263 3.8 3.6 1.0
CZ3 A:TRP226 3.8 16.7 1.0
CD1 A:LEU263 3.9 17.2 1.0
CD A:GLN227 3.9 21.9 1.0
CE2 A:TRP223 3.9 11.4 1.0
CA A:ALA234 4.1 20.4 1.0
C A:ALA234 4.3 17.2 1.0
CG A:LEU263 4.3 13.1 1.0
CB A:TYR235 4.3 5.5 1.0
CA A:TYR235 4.5 11.0 1.0
CB A:ALA234 4.5 24.1 1.0
CH2 A:TRP226 4.6 20.7 1.0
CD1 A:TRP223 4.6 4.7 1.0
CB A:LEU263 4.6 11.6 1.0
CD2 A:TYR165 4.7 16.6 1.0
CE3 A:TRP226 4.8 18.5 1.0
CE2 A:TYR165 4.8 16.4 1.0
CH2 A:TRP223 4.9 10.3 1.0

Reference:

Y.Matoba, T.Kumagai, A.Yamamoto, H.Yoshitsu, M.Sugiyama. Crystallographic Evidence That the Dinuclear Copper Center of Tyrosinase Is Flexible During Catalysis J.Biol.Chem. V. 281 8981 2006.
ISSN: ISSN 0021-9258
PubMed: 16436386
DOI: 10.1074/JBC.M509785200
Page generated: Sat Dec 12 08:54:13 2020

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