Chlorine in PDB 230l: T4 Lysozyme Mutant M6L

All present enzymatic activity of T4 Lysozyme Mutant M6L:
3.2.1.17;

The structure of T4 Lysozyme Mutant M6L, PDB code: 230l was solved by L.A.Lipscomb, N.C.Gassner, S.Snow, A.M.Eldridge, D.L.Drew, W.A.Baase, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	60.870, 60.870, 97.200, 90.00, 90.00, 120.00
R / Rfree (%)	18.4 / n/a

The binding sites of Chlorine atom in the T4 Lysozyme Mutant M6L (pdb code 230l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the T4 Lysozyme Mutant M6L, PDB code: 230l:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the T4 Lysozyme Mutant M6L


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of T4 Lysozyme Mutant M6L within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl205 b:14.8 occ:1.00 N A:ARG145 3.0 6.9 1.0 O A:HOH363 3.0 60.0 1.0 N A:ASN144 3.4 3.8 1.0 C A:THR142 3.4 13.6 1.0 CB A:THR142 3.4 12.4 1.0 CA A:THR142 3.5 13.0 1.0 CB A:ARG145 3.5 6.7 1.0 O A:THR142 3.6 10.2 1.0 CB A:ASN144 3.8 6.0 1.0 CA A:ASN144 3.8 6.1 1.0 CA A:ARG145 3.8 8.9 1.0 C A:ASN144 3.9 10.8 1.0 N A:PRO143 3.9 17.2 1.0 C A:PRO143 4.2 9.6 1.0 CG2 A:THR142 4.2 9.5 1.0 O A:HOH256 4.3 14.7 0.5 CD A:PRO143 4.5 8.6 1.0 OG1 A:THR142 4.5 12.8 1.0 CA A:PRO143 4.6 13.2 1.0 CG A:ASN144 4.7 33.6 1.0 N A:ALA146 4.9 8.9 1.0 C A:ARG145 4.9 6.8 1.0 N A:THR142 5.0 8.0 1.0 CG A:ARG145 5.0 12.4 1.0

Chlorine binding site 2 out of 2 in the T4 Lysozyme Mutant M6L


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of T4 Lysozyme Mutant M6L within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl242 b:29.6 occ:0.50 O A:HOH211 3.0 17.0 1.0 O A:HOH226 3.1 27.2 1.0 O A:HOH214 3.4 20.9 1.0 CB A:ALA49 3.8 16.3 1.0 CE1 A:HIS31 3.9 7.3 1.0 NE2 A:HIS31 4.0 9.8 1.0 CA A:ALA49 4.2 16.0 1.0 CD2 A:LEU66 4.3 20.5 1.0 NE2 A:GLN69 4.4 19.1 1.0 O A:ALA49 4.7 18.5 1.0 C A:ALA49 5.0 14.3 1.0

L.A.Lipscomb, N.C.Gassner, S.D.Snow, A.M.Eldridge, W.A.Baase, D.L.Drew, B.W.Matthews. Context-Dependent Protein Stabilization By Methionine-to-Leucine Substitution Shown in T4 Lysozyme. Protein Sci. V. 7 765 1998.
ISSN: ISSN 0961-8368
PubMed: 9541409