Chlorine in PDB 258l: An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme

All present enzymatic activity of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme:
3.2.1.17;

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 258l was solved by J.W.Wray, W.A.Baase, G.J.Ostheimer, B.W.Matthews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	61.330, 61.330, 97.470, 90.00, 90.00, 120.00
R / Rfree (%)	17.3 / n/a

The structure of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Chlorine atom in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme (pdb code 258l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme, PDB code: 258l:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl173 b:31.3 occ:1.00 O A:HOH200 3.0 28.3 1.0 N A:ARG145 3.1 13.8 1.0 C A:HIS142 3.5 13.8 1.0 N A:ASN144 3.5 6.7 1.0 CA A:HIS142 3.5 10.6 1.0 CB A:HIS142 3.7 12.0 1.0 O A:HIS142 3.7 10.7 1.0 CB A:ARG145 3.7 9.4 1.0 CA A:ASN144 3.9 9.6 1.0 CB A:ASN144 4.0 7.7 1.0 C A:ASN144 4.0 18.2 1.0 N A:PRO143 4.0 11.1 1.0 CA A:ARG145 4.0 9.2 1.0 CD A:PRO143 4.4 15.5 1.0 C A:PRO143 4.4 11.5 1.0 O A:HOH214 4.5 47.6 1.0 O A:HOH220 4.8 28.9 1.0 CA A:PRO143 4.8 9.9 1.0 CG A:HIS142 4.9 15.3 1.0 ND2 A:ASN144 5.0 20.9 1.0 N A:HIS142 5.0 11.0 1.0 CG A:ASN144 5.0 26.3 1.0

Chlorine binding site 2 out of 2 in the An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of An Adaptable Metal-Binding Site Engineered Into T4 Lysozyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl178 b:17.1 occ:0.50 O A:ALA49 2.9 18.3 1.0 NE2 A:GLN69 3.0 19.5 1.0 O A:HOH260 3.1 21.8 1.0 C A:ALA49 3.8 11.4 1.0 CD A:GLN69 3.8 25.7 1.0 OE1 A:GLN69 3.8 23.6 1.0 O A:HOH222 3.9 39.2 1.0 CA A:ALA49 3.9 13.9 1.0 O A:HOH183 4.0 44.2 1.0 CB A:ALA49 4.1 11.3 1.0 CD2 A:LEU66 4.1 24.2 1.0 O A:HOH182 4.3 20.1 1.0 N A:ILE50 5.0 10.0 1.0

J.W.Wray, W.A.Baase, G.J.Ostheimer, X.J.Zhang, B.W.Matthews. Use of A Non-Rigid Region in T4 Lysozyme to Design An Adaptable Metal-Binding Site. Protein Eng. V. 13 313 2000.
ISSN: ISSN 0269-2139
PubMed: 10835104
DOI: 10.1093/PROTEIN/13.5.313