Chlorine in PDB 2a53: Fluorescent Protein ASFP595, A143S, Off-State

The structure of Fluorescent Protein ASFP595, A143S, Off-State, PDB code: 2a53 was solved by M.Andresen, M.C.Wahl, A.C.Stiel, F.Graeter, L.Schaefer, S.Trowitzsch, G.Weber, C.Eggeling, H.Grubmueller, S.W.Hell, S.Jakobs, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.45
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	75.996, 126.259, 93.765, 90.00, 90.00, 90.00
R / Rfree (%)	17.3 / 19.9

The binding sites of Chlorine atom in the Fluorescent Protein ASFP595, A143S, Off-State (pdb code 2a53). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Fluorescent Protein ASFP595, A143S, Off-State, PDB code: 2a53:

Chlorine binding site 1 out of 1 in the Fluorescent Protein ASFP595, A143S, Off-State


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Fluorescent Protein ASFP595, A143S, Off-State within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl601 b:21.8 occ:1.00 N B:PHE192 3.2 12.6 1.0 O B:HOH661 3.5 20.8 1.0 CA B:GLY191 3.7 14.3 1.0 CB B:PHE192 3.9 12.4 1.0 C B:GLY191 4.0 14.1 1.0 CA B:PHE192 4.1 12.5 1.0 O B:HOH713 4.3 42.6 1.0 O B:HOH647 4.4 17.4 1.0 O B:HOH758 4.7 30.7 1.0 O B:HOH739 4.9 37.8 1.0

M.Andresen, M.C.Wahl, A.C.Stiel, F.Graeter, L.Schaefer, S.Trowitzsch, G.Weber, C.Eggeling, H.Grubmueller, S.W.Hell, S.Jakobs. Structure and Mechanism of the Reversible Photoswitch of A Fluorescent Protein Proc.Natl.Acad.Sci.Usa V. 102 13070 2005.
ISSN: ISSN 0027-8424
PubMed: 16135569
DOI: 10.1073/PNAS.0502772102