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Chlorine in PDB 2bg2: Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced.
Enzymatic activity of Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced.
All present enzymatic activity of Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced.:
3.5.2.6;
3.5.2.6;
Protein crystallography data
The structure of Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced., PDB code: 2bg2
was solved by
A.M.Davies,
R.M.Rasia,
A.J.Vila,
B.J.Sutton,
S.M.Fabiane,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 6.00 / 2.40 |
| Space group | P 31 2 1 |
| Cell size a, b, c (Å), α, β, γ (°) | 67.300, 67.300, 177.183, 90.00, 90.00, 120.00 |
| R / Rfree (%) | 17.9 / 22.4 |
Other elements in 2bg2:
The structure of Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced. also contains other interesting chemical elements:
| Zinc | (Zn) | 4 atoms |
Chlorine Binding Sites:
The binding sites of Chlorine atom in the Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced.
(pdb code 2bg2). This binding sites where shown within
5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced., PDB code: 2bg2:
In total only one binding site of Chlorine was determined in the Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced., PDB code: 2bg2:
Chlorine binding site 1 out of 1 in 2bg2
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Chlorine Binding Sites List in 2bg2
Chlorine binding site 1 out
of 1 in the Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced.
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Chlorine with other atoms in the Cl binding
site number 1 of Bacillus Cereus Metallo-Beta-Lactamase (Bcii) Arg (121) Cys Mutant. Solved at PH4.5 Using 20MM ZNSO4 in the Buffer. 1MM Dtt and 1MM Tcep- Hcl Were Used As Reducing Agents. CYS221 Is Reduced. within 5.0Å range:
|
Reference:
A.M.Davies,
R.M.Rasia,
A.J.Vila,
B.J.Sutton,
S.M.Fabiane.
Effect of pH on the Active Site of An ARG121CYS Mutant of the Metallo-Beta-Lactamase From Bacillus Cereus: Implications For the Enzyme Mechanism. Biochemistry V. 44 4841 2005.
ISSN: ISSN 0006-2960
PubMed: 15779910
DOI: 10.1021/BI047709T
Page generated: Sat Dec 12 09:00:08 2020
ISSN: ISSN 0006-2960
PubMed: 15779910
DOI: 10.1021/BI047709T
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