Atomistry » Chlorine » PDB 2dxa-2ess » 2e0l
Atomistry »
  Chlorine »
    PDB 2dxa-2ess »
      2e0l »

Chlorine in PDB 2e0l: Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)

Enzymatic activity of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)

All present enzymatic activity of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L):
3.1.27.5;

Protein crystallography data

The structure of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L), PDB code: 2e0l was solved by H.Yamada, T.Tamada, M.Kosaka, R.Kuroki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.60
Space group I 41
Cell size a, b, c (Å), α, β, γ (°) 93.376, 93.376, 93.009, 90.00, 90.00, 90.00
R / Rfree (%) 19.4 / 21.5

Other elements in 2e0l:

The structure of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) also contains other interesting chemical elements:

Cadmium (Cd) 8 atoms

Chlorine Binding Sites:

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 11;

Binding sites:

The binding sites of Chlorine atom in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) (pdb code 2e0l). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 11 binding sites of Chlorine where determined in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L), PDB code: 2e0l:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Chlorine binding site 1 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 1 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl2001

b:29.0
occ:1.00
 CD A:CD1002 2.5 18.5 0.5
CL A:CL2002 3.5 19.9 1.0

Chlorine binding site 2 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 2 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl2002

b:19.9
occ:1.00
 CD A:CD1002 2.3 18.5 0.5
N A:GLU125 3.2 31.8 1.0
CL A:CL2001 3.5 29.0 1.0
NE A:ARG104 3.7 30.1 1.0
CA A:VAL124 3.8 31.9 1.0
CB A:GLU125 3.9 30.8 1.0
C A:VAL124 3.9 31.9 1.0
O A:HOH2035 4.0 27.6 1.0
CD A:ARG104 4.1 30.1 1.0
CA A:GLU125 4.2 31.9 1.0
CZ A:ARG104 4.3 32.2 1.0
O A:SER123 4.3 32.1 1.0
NH2 A:ARG104 4.4 34.3 1.0
CB A:VAL124 4.6 33.5 1.0
N A:VAL124 4.8 32.0 1.0
C A:SER123 4.9 32.4 1.0
OE2 A:GLU125 5.0 29.1 1.0

Chlorine binding site 3 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 3 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl2005

b:35.9
occ:1.00
 CD A:CD1004 2.5 34.5 1.0
NE A:ARG85 3.4 44.6 1.0
NZ A:LYS102 3.6 39.0 1.0
CB A:SER100 3.6 30.0 1.0
OD2 A:ASP83 3.6 40.1 1.0
OG A:SER100 3.7 34.1 1.0
CB A:ASP83 3.7 32.3 1.0
CD A:ARG85 3.8 35.6 1.0
CL A:CL2006 4.1 28.2 1.0
CG A:ASP83 4.1 36.5 1.0
CZ A:ARG85 4.4 47.1 1.0
CE A:LYS102 4.5 41.4 1.0
CG A:ARG85 4.6 34.9 1.0
NH2 A:ARG85 4.6 51.0 1.0

Chlorine binding site 4 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 4 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl2006

b:28.2
occ:1.00
 CD A:CD1004 2.5 34.5 1.0
CD A:ARG85 3.6 35.6 1.0
O A:HOH2165 3.9 42.0 1.0
CL A:CL2005 4.1 35.9 1.0
NE A:ARG85 4.2 44.6 1.0
O A:HOH2192 4.6 47.7 1.0
CG A:ARG85 4.9 34.9 1.0
CZ A:ARG85 4.9 47.1 1.0

Chlorine binding site 5 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 5 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl2009

b:43.3
occ:1.00
 CD A:CD1006 2.7 70.6 1.0
O A:HOH2063 2.7 25.1 1.0
N A:PHE120 3.0 32.2 1.0
NE2 A:HIS12 3.1 31.9 1.0
O A:HOH2146 3.2 41.8 1.0
O A:HOH2090 3.4 42.8 1.0
CA A:HIS119 3.4 32.6 1.0
O A:HOH2153 3.7 48.8 1.0
CD2 A:HIS12 3.7 32.1 1.0
C A:HIS119 3.7 32.6 1.0
ND1 A:HIS119 3.8 36.1 1.0
CA A:PHE120 4.0 32.2 1.0
CE1 A:HIS12 4.1 33.9 1.0
OE1 A:GLN11 4.1 35.1 1.0
CB A:PHE120 4.1 32.9 1.0
CB A:HIS119 4.2 33.1 1.0
O A:PHE120 4.2 32.4 1.0
CG A:HIS119 4.3 35.5 1.0
O A:VAL118 4.4 33.4 1.0
O A:HOH2188 4.4 55.8 1.0
N A:HIS119 4.5 32.8 1.0
C A:PHE120 4.6 32.5 1.0
O A:HOH2034 4.7 29.3 1.0
CE1 A:HIS119 4.7 37.1 1.0
CD1 A:PHE8 4.8 31.0 1.0
C A:VAL118 4.9 31.7 1.0
CG A:HIS12 4.9 32.4 1.0
O A:HIS119 4.9 32.8 1.0

Chlorine binding site 6 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 6 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl2011

b:34.0
occ:1.00
 CD A:CD1008 2.3 36.7 1.0
O A:HOH2019 2.6 20.9 1.0
O A:HOH2096 2.9 37.2 1.0
OE1 A:GLU49 3.0 32.8 1.0
O A:HOH2033 3.3 34.1 1.0
O A:HOH2132 3.3 35.3 1.0
ND1 A:HIS80 3.4 34.8 1.0
CE1 A:HIS80 3.4 30.8 1.0
CD A:GLU49 3.8 32.6 1.0
OE2 A:GLU49 3.8 32.6 1.0
O A:SER78 3.8 35.5 1.0
O A:HOH2158 4.2 47.1 1.0
CG A:HIS80 4.4 30.2 1.0
N A:HIS80 4.4 28.1 1.0
NE2 A:HIS80 4.4 35.1 1.0
CA A:MET79 4.5 30.4 1.0
O A:HOH2122 4.7 40.3 1.0
OE1 A:GLU103 4.8 36.5 1.0
O A:HOH2182 4.8 40.2 1.0
C A:MET79 4.9 31.2 1.0
C A:SER78 4.9 32.5 1.0
CD2 A:HIS80 4.9 32.6 1.0

Chlorine binding site 7 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 7 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl2003

b:29.3
occ:1.00
 CD B:CD1003 3.1 18.5 0.5
CL B:CL2004 3.5 20.3 1.0

Chlorine binding site 8 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 8 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 8 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl2004

b:20.3
occ:1.00
 CD B:CD1003 2.8 18.5 0.5
N B:GLU125 3.2 32.4 1.0
CL B:CL2003 3.5 29.3 1.0
NE B:ARG104 3.6 31.1 1.0
CA B:VAL124 3.8 32.2 1.0
CB B:GLU125 3.8 31.6 1.0
C B:VAL124 3.9 32.2 1.0
O B:HOH2198 4.0 27.7 1.0
CA B:GLU125 4.1 32.7 1.0
CD B:ARG104 4.1 31.7 1.0
CZ B:ARG104 4.2 33.8 1.0
O B:SER123 4.3 32.7 1.0
NH2 B:ARG104 4.4 36.0 1.0
CB B:VAL124 4.6 33.7 1.0
N B:VAL124 4.8 32.8 1.0
OE2 B:GLU125 4.9 29.9 1.0
C B:SER123 4.9 33.0 1.0

Chlorine binding site 9 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 9 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 9 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl2007

b:35.6
occ:1.00
 CD B:CD1005 2.3 36.7 1.0
O B:HOH2021 2.6 21.8 1.0
O B:HOH2097 2.9 36.4 1.0
OE1 B:GLU49 3.0 32.7 1.0
O B:HOH2093 3.2 37.0 1.0
ND1 B:HIS80 3.3 34.4 1.0
CL B:CL2008 3.5 48.1 1.0
CE1 B:HIS80 3.5 30.8 1.0
CD B:GLU49 3.8 33.2 1.0
OE2 B:GLU49 3.8 32.4 1.0
O B:SER78 3.8 35.5 1.0
O B:HOH2054 3.9 26.1 1.0
N B:HIS80 4.4 28.4 1.0
CG B:HIS80 4.4 29.8 1.0
CA B:MET79 4.5 30.9 1.0
NE2 B:HIS80 4.6 34.6 1.0
O B:HOH2122 4.7 35.6 1.0
O B:HOH2131 4.8 42.7 1.0
O B:HOH2189 4.8 42.0 1.0
C B:MET79 4.8 30.7 1.0
C B:SER78 4.9 32.7 1.0

Chlorine binding site 10 out of 11 in 2e0l

Go back to Chlorine Binding Sites List in 2e0l
Chlorine binding site 10 out of 11 in the Mutant Human Ribonuclease 1 (Q28L, R31L, R32L)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 10 of Mutant Human Ribonuclease 1 (Q28L, R31L, R32L) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl2008

b:48.1
occ:1.00
 CD B:CD1005 2.7 36.7 1.0
O B:HOH2093 2.7 37.0 1.0
CE1 B:HIS80 3.2 30.8 1.0
CL B:CL2007 3.5 35.6 1.0
ND1 B:HIS80 3.6 34.4 1.0
O B:HOH2122 3.7 35.6 1.0
NE2 B:HIS80 4.5 34.6 1.0
O B:HOH2177 4.7 61.1 1.0
O B:HOH2172 4.8 57.1 1.0
OE2 B:GLU49 4.9 32.4 1.0
CG B:HIS80 5.0 29.8 1.0

Reference:

H.Yamada, T.Tamada, M.Kosaka, K.Miyata, S.Fujiki, M.Tano, M.Moriya, M.Yamanishi, E.Honjo, H.Tada, T.Ino, H.Yamaguchi, J.Futami, M.Seno, T.Nomoto, T.Hirata, M.Yoshimura, R.Kuroki. 'Crystal Lattice Engineering,' An Approach to Engineer Protein Crystal Contacts By Creating Intermolecular Symmetry: Crystallization and Structure Determination of A Mutant Human Rnase 1 with A Hydrophobic Interface of Leucines Protein Sci. V. 16 1389 2007.
ISSN: ISSN 0961-8368
PubMed: 17586772
DOI: 10.1110/PS.072851407
Page generated: Sat Jul 20 06:42:46 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy