Chlorine in PDB 2he3: Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2)

All present enzymatic activity of Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2):
1.11.1.9;

The structure of Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2), PDB code: 2he3 was solved by C.Johansson, K.L.Kavanagh, A.Rojkova, O.Gileadi, F.Von Delft, C.Arrowsmith, J.Weigelt, M.Sundstrom, A.Edwards, U.Oppermann, Structuralgenomics Consortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.00 / 2.10
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	41.308, 117.212, 120.157, 90.00, 90.00, 90.00
R / Rfree (%)	15.6 / 20.5

The binding sites of Chlorine atom in the Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2) (pdb code 2he3). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2), PDB code: 2he3:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl189 b:23.0 occ:1.00 O A:HOH196 3.2 20.9 1.0 N A:ARG59 3.2 22.4 1.0 NE A:ARG60 3.4 30.9 1.0 N A:ARG60 3.4 21.5 1.0 CD A:PRO58 3.5 16.6 1.0 N A:PRO58 3.5 17.9 1.0 C A:PHE57 3.6 21.6 1.0 CA A:PHE57 3.7 19.8 1.0 CG A:ARG60 3.7 21.6 1.0 CB A:ARG59 3.8 21.1 1.0 CA A:ARG59 3.8 23.4 1.0 CB A:ARG60 4.0 14.2 1.0 C A:ARG59 4.1 22.0 1.0 CG A:PRO58 4.1 20.2 1.0 CD A:ARG60 4.1 28.1 1.0 O A:PHE57 4.1 18.8 1.0 C A:PRO58 4.2 20.7 1.0 CA A:ARG60 4.3 19.9 1.0 CZ A:ARG60 4.4 34.2 1.0 CB A:PHE57 4.4 26.8 1.0 NH2 A:ARG60 4.4 29.5 1.0 CA A:PRO58 4.4 17.3 1.0 CG A:ARG59 4.5 25.2 1.0 O A:HOH260 4.5 37.3 1.0 O A:ARG56 4.6 18.7 1.0 N A:PHE57 4.8 20.1 1.0 CB A:PRO58 5.0 16.9 1.0 CD2 A:PHE57 5.0 20.6 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl190 b:38.0 occ:1.00 N A:GLU162 3.2 23.2 1.0 O A:HOH289 3.3 37.6 1.0 CA A:GLY160 3.4 18.8 1.0 N A:GLU164 3.4 21.6 1.0 C A:GLY160 3.5 19.4 1.0 CB A:GLU162 3.5 29.6 1.0 N A:GLY163 3.6 18.4 1.0 CA A:GLU162 3.6 26.1 1.0 OE1 A:GLU162 3.6 67.2 1.0 CB A:GLU164 3.6 24.3 1.0 C A:GLU162 3.7 23.5 1.0 N A:PRO161 3.8 20.9 1.0 O A:GLY160 3.8 20.9 1.0 O A:GLU164 3.9 16.2 1.0 CA A:GLU164 4.0 23.0 1.0 N A:GLY160 4.0 19.2 1.0 CD A:PRO161 4.2 20.5 1.0 C A:PRO161 4.3 22.8 1.0 CD A:GLU162 4.4 56.9 1.0 C A:GLU164 4.5 21.1 1.0 C A:GLY163 4.5 19.4 1.0 O A:GLU162 4.5 24.2 1.0 CA A:GLY163 4.6 20.8 1.0 CG A:GLU162 4.6 35.8 1.0 CA A:PRO161 4.7 24.6 1.0 CG A:PRO161 5.0 24.4 1.0

K.L.Kavanagh, U.Oppermann. Crystal Structure of the Selenocysteine to Cysteine Mutant of Human Glutathionine Peroxidase 2 (GPX2) To Be Published.