Chlorine in PDB 2odi: Restriction Endonuclease Bcni-Cognate Dna Substrate Complex

The structure of Restriction Endonuclease Bcni-Cognate Dna Substrate Complex, PDB code: 2odi was solved by M.Sokolowska, M.Kaus-Drobek, H.Czapinska, G.Tamulaitis, R.H.Szczepanowski, C.Urbanke, V.Siksnys, M.Bochtler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.38 / 1.45
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	81.553, 87.870, 79.391, 90.00, 90.00, 90.00
R / Rfree (%)	17.4 / 19.9

The structure of Restriction Endonuclease Bcni-Cognate Dna Substrate Complex also contains other interesting chemical elements:

Calcium

(Ca)

3 atoms

The binding sites of Chlorine atom in the Restriction Endonuclease Bcni-Cognate Dna Substrate Complex (pdb code 2odi). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Restriction Endonuclease Bcni-Cognate Dna Substrate Complex, PDB code: 2odi:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Restriction Endonuclease Bcni-Cognate Dna Substrate Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Restriction Endonuclease Bcni-Cognate Dna Substrate Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl705 b:18.6 occ:1.00 O A:HOH861 3.0 17.4 1.0 O C:HOH43 3.1 31.0 1.0 O A:HOH726 3.1 10.7 1.0 N A:VAL86 3.2 9.5 1.0 CG2 A:VAL86 3.6 12.0 1.0 O C:HOH14 3.6 28.1 1.0 CB A:THR85 3.7 9.6 1.0 CA A:THR85 3.7 9.6 1.0 CD A:PRO80 3.8 8.4 1.0 CB A:LYS79 3.9 10.1 1.0 C A:THR85 3.9 9.5 1.0 CB A:VAL86 4.0 9.5 1.0 CG A:PRO80 4.1 9.2 1.0 CA A:VAL86 4.2 9.9 1.0 CG2 A:THR85 4.3 10.0 1.0 OP2 C:DA-3 4.3 16.2 1.0 CD A:LYS79 4.4 14.9 1.0 O A:HOH771 4.4 17.0 1.0 O A:HOH896 4.5 28.1 1.0 N A:PRO80 4.6 9.0 1.0 CG A:LYS79 4.7 11.4 1.0 CA A:LYS79 4.7 9.3 1.0 OG1 A:THR85 4.8 10.2 1.0 O A:HOH734 4.9 15.5 1.0 O A:PRO80 4.9 9.0 1.0

Chlorine binding site 2 out of 2 in the Restriction Endonuclease Bcni-Cognate Dna Substrate Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Restriction Endonuclease Bcni-Cognate Dna Substrate Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl704 b:15.2 occ:1.00 O B:HOH883 3.1 27.9 1.0 O B:HOH714 3.1 13.4 1.0 O E:HOH482 3.2 32.0 1.0 N B:VAL86 3.3 10.7 1.0 CG2 B:VAL86 3.7 12.9 1.0 CB B:THR85 3.7 10.6 1.0 CA B:THR85 3.8 10.7 1.0 CD B:PRO80 3.8 9.1 1.0 CE B:LYS79 3.8 13.8 0.5 CG B:PRO80 4.0 10.0 1.0 CB B:VAL86 4.0 11.5 1.0 CB B:LYS79 4.0 10.0 1.0 O E:HOH224 4.0 27.7 1.0 C B:THR85 4.0 10.6 1.0 CA B:VAL86 4.2 11.4 1.0 CG2 B:THR85 4.3 11.7 1.0 CD B:LYS79 4.4 14.8 0.5 O B:HOH731 4.5 18.0 1.0 CD B:LYS79 4.6 11.7 0.5 NZ B:LYS79 4.6 15.4 0.5 O B:HOH844 4.7 25.1 1.0 OP2 E:DA-3 4.7 15.0 1.0 N B:PRO80 4.7 9.4 1.0 CG B:LYS79 4.8 11.5 1.0 OG1 B:THR85 4.8 12.2 1.0 CA B:LYS79 4.9 9.7 1.0 O B:PRO80 4.9 10.2 1.0

M.Sokolowska, M.Kaus-Drobek, H.Czapinska, G.Tamulaitis, R.H.Szczepanowski, C.Urbanke, V.Siksnys, M.Bochtler. Monomeric Restriction Endonuclease Bcni in the Apo Form and in An Asymmetric Complex with Target Dna. J.Mol.Biol. V. 369 722 2007.
ISSN: ISSN 0022-2836
PubMed: 17445830
DOI: 10.1016/J.JMB.2007.03.018