Chlorine in PDB 2pu3: Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida

All present enzymatic activity of Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida:
3.1.21.1;

The structure of Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida, PDB code: 2pu3 was solved by B.Altermark, R.Helland, E.Moe, N.P.Willassen, A.O.Smalas, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.92 / 1.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	42.078, 44.948, 51.746, 90.00, 92.66, 90.00
R / Rfree (%)	16.6 / 18.5

The structure of Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Chlorine atom in the Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida (pdb code 2pu3). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida, PDB code: 2pu3:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl301 b:5.3 occ:1.00 OG A:SER41 3.1 6.0 1.0 N A:CYS44 3.3 5.9 1.0 N A:ILE123 3.3 4.2 1.0 N A:TYR43 3.3 4.3 1.0 CB A:SER41 3.5 5.9 1.0 CB A:TYR43 3.6 5.1 1.0 CA A:ALA122 3.8 3.8 1.0 CA A:TYR43 3.8 4.7 1.0 CG1 A:ILE123 3.8 5.4 1.0 CB A:CYS44 3.9 6.3 1.0 N A:PHE42 3.9 5.1 1.0 C A:TYR43 4.0 5.5 1.0 CB A:ILE123 4.0 4.4 1.0 C A:ALA122 4.0 4.3 1.0 CD1 A:ILE123 4.1 7.4 1.0 CD1 A:TYR43 4.1 3.6 1.0 C A:SER41 4.1 5.6 1.0 CB A:ALA122 4.1 4.3 1.0 SG A:CYS44 4.1 8.4 1.0 CA A:CYS44 4.2 6.4 1.0 CA A:ILE123 4.3 4.3 1.0 CG A:TYR43 4.3 3.5 1.0 C A:PHE42 4.3 4.8 1.0 CA A:SER41 4.4 5.8 1.0 O A:PRO121 4.6 5.2 1.0 CA A:PHE42 4.6 4.8 1.0 O A:SER41 4.6 5.8 1.0 CB A:VAL126 4.9 4.1 1.0 CG2 A:VAL126 4.9 5.2 1.0 N A:GLY45 4.9 5.8 1.0 N A:ALA122 5.0 4.3 1.0

Chlorine binding site 2 out of 2 in the Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:10.4 occ:1.00 O A:HOH308 3.2 10.6 1.0 O A:HOH401 3.2 24.2 1.0 N A:SER186 3.3 9.9 1.0 NE2 A:GLN86 3.5 13.7 1.0 OG A:SER186 3.6 10.5 1.0 CB A:SER186 3.6 9.9 1.0 CG A:GLN189 3.7 6.7 1.0 CA A:SER186 4.1 9.8 1.0 CA A:LEU185 4.2 10.7 1.0 CB A:GLN189 4.2 6.5 1.0 CD1 A:LEU185 4.3 12.7 1.0 C A:LEU185 4.3 10.4 1.0 CD A:GLN86 4.4 10.2 1.0 ND2 A:ASN184 4.7 14.6 1.0 O A:HOH392 4.7 14.6 1.0 OE1 A:GLN86 4.7 10.1 1.0 CD A:GLN189 4.7 6.7 1.0 O A:HOH448 4.8 17.9 1.0 O A:ASN184 4.8 10.7 1.0 OE1 A:GLN189 4.9 7.8 1.0 CG A:LEU185 5.0 11.5 1.0

B.Altermark, R.Helland, E.Moe, N.P.Willassen, A.O.Smalas. Structural Adaptation of Endonuclease I From the Cold-Adapted and Halophilic Bacterium Vibrio Salmonicida. Acta Crystallogr.,Sect.D V. 64 368 2008.
ISSN: ISSN 0907-4449
PubMed: 18391403
DOI: 10.1107/S0907444908000097