Chlorine in PDB 2q10: Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex

The structure of Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex, PDB code: 2q10 was solved by M.Sokolowska, M.Kaus-Drobek, H.Czapinska, G.Tamulaitis, R.H.Szczepanowski, C.Urbanke, V.Siksnys, M.Bochtler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.72 / 1.75
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	81.354, 87.551, 79.162, 90.00, 90.00, 90.00
R / Rfree (%)	17.4 / 21.3

The structure of Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex also contains other interesting chemical elements:

Calcium

(Ca)

3 atoms

The binding sites of Chlorine atom in the Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex (pdb code 2q10). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex, PDB code: 2q10:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl705 b:17.7 occ:1.00 O A:HOH858 2.9 16.1 1.0 O A:HOH724 3.1 7.2 1.0 O C:HOH739 3.2 31.5 1.0 N A:VAL86 3.2 8.3 1.0 O C:HOH715 3.3 24.2 1.0 CG2 A:VAL86 3.6 9.9 1.0 CA A:THR85 3.7 7.6 1.0 CB A:THR85 3.7 7.8 1.0 CD A:PRO80 3.7 8.4 1.0 CB A:LYS79 3.9 8.8 1.0 C A:THR85 3.9 7.6 1.0 CB A:VAL86 4.0 8.6 1.0 CG A:PRO80 4.0 9.1 1.0 CA A:VAL86 4.2 7.9 1.0 CG2 A:THR85 4.2 7.2 1.0 O A:HOH890 4.4 21.6 1.0 CD A:LYS79 4.4 11.1 1.0 O A:HOH767 4.5 9.3 1.0 OP2 C:DA-3 4.5 13.2 1.0 N A:PRO80 4.6 8.6 1.0 CA A:LYS79 4.7 8.5 1.0 O A:HOH731 4.7 10.6 1.0 CG A:LYS79 4.8 9.1 1.0 O A:PRO80 4.8 5.8 1.0 OG1 A:THR85 4.9 7.2 1.0

Chlorine binding site 2 out of 2 in the Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Restriction Endonuclease Bcni (Wild Type)-Cognate Dna Substrate Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl704 b:12.9 occ:1.00 O B:HOH881 3.2 25.6 1.0 O B:HOH713 3.2 8.7 1.0 O E:HOH482 3.3 26.8 1.0 N B:VAL86 3.3 9.4 1.0 CG2 B:VAL86 3.7 9.6 1.0 CD B:PRO80 3.8 8.6 1.0 CB B:THR85 3.8 8.7 1.0 O E:HOH224 3.8 22.4 1.0 CA B:THR85 3.9 9.1 1.0 CE B:LYS79 4.0 16.3 1.0 CB B:LYS79 4.0 9.5 1.0 CB B:VAL86 4.0 9.6 1.0 CG B:PRO80 4.0 8.5 1.0 C B:THR85 4.1 8.5 1.0 CA B:VAL86 4.2 9.1 1.0 O B:HOH731 4.5 14.0 1.0 CG2 B:THR85 4.5 8.6 1.0 CD B:LYS79 4.6 12.0 1.0 N B:PRO80 4.6 8.4 1.0 CA B:LYS79 4.8 9.1 1.0 CG B:LYS79 4.8 9.9 1.0 NZ B:LYS79 4.8 20.0 1.0 OG1 B:THR85 4.9 9.5 1.0 O B:PRO80 4.9 8.7 1.0 OP2 E:DA-3 4.9 12.8 1.0 O B:HOH733 5.0 10.1 1.0 O B:HOH843 5.0 29.4 1.0

M.Sokolowska, M.Kaus-Drobek, H.Czapinska, G.Tamulaitis, R.H.Szczepanowski, C.Urbanke, V.Siksnys, M.Bochtler. Monomeric Restriction Endonuclease Bcni in the Apo Form and in An Asymmetric Complex with Target Dna. J.Mol.Biol. V. 369 722 2007.
ISSN: ISSN 0022-2836
PubMed: 17445830
DOI: 10.1016/J.JMB.2007.03.018