Chlorine in PDB 2v9k: Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase.

The structure of Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase., PDB code: 2v9k was solved by C.J.Mccleverty, M.Hornsby, G.Spraggon, A.Kreusch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.84 / 2.0
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	80.611, 80.611, 153.947, 90.00, 90.00, 120.00
R / Rfree (%)	18.6 / 23

The structure of Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase. also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Chlorine atom in the Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase. (pdb code 2v9k). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase., PDB code: 2v9k:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase. within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1529 b:36.9 occ:1.00 O A:HOH2206 2.9 38.0 1.0 NH1 A:ARG447 3.0 45.6 1.0 N A:ILE486 3.1 36.5 1.0 N A:LYS487 3.3 34.0 1.0 C3 A:EPE1534 3.4 45.1 1.0 C7 A:EPE1534 3.6 38.1 1.0 C A:TYR485 3.8 36.2 1.0 CA A:TYR485 3.8 36.0 1.0 CG A:LYS487 3.8 28.4 1.0 CA A:ILE486 3.9 35.3 1.0 N4 A:EPE1534 4.0 43.4 1.0 CB A:LYS487 4.0 31.2 1.0 C A:ILE486 4.1 36.3 1.0 CB A:ILE486 4.1 35.0 1.0 OE1 A:GLU488 4.1 40.0 1.0 O A:HOH2205 4.1 41.7 1.0 CD2 A:TYR485 4.2 35.8 1.0 CZ A:ARG447 4.2 46.0 1.0 CB A:TYR485 4.2 35.0 1.0 CA A:LYS487 4.2 33.2 1.0 CD2 A:LEU514 4.3 32.2 1.0 CD A:LYS487 4.3 35.1 1.0 CG1 A:ILE486 4.5 35.7 1.0 O8 A:EPE1534 4.5 28.8 1.0 C8 A:EPE1534 4.6 33.4 1.0 OD1 A:ASP344 4.6 42.5 1.0 CG A:TYR485 4.7 37.9 1.0 C2 A:EPE1534 4.7 48.1 1.0 O A:TYR485 4.8 34.0 1.0 NH2 A:ARG447 4.8 50.9 1.0 CE A:LYS487 4.9 31.9 1.0 O A:THR484 4.9 38.0 1.0

Chlorine binding site 2 out of 4 in the Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase. within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1530 b:40.6 occ:1.00 O A:HOH2265 3.0 34.7 1.0 O A:HOH2316 3.3 41.7 1.0 NH1 A:ARG354 3.3 27.3 1.0 O A:HOH2264 3.3 42.3 1.0 CA A:GLY341 3.6 27.9 1.0 N A:ARG342 3.9 27.6 1.0 CE A:LYS412 3.9 31.9 1.0 C6 A:EPE1534 4.0 47.9 1.0 C5 A:EPE1534 4.0 46.9 1.0 CD A:LYS412 4.1 30.9 1.0 O A:ARG342 4.1 32.4 1.0 O A:HOH2199 4.2 34.2 1.0 C A:GLY341 4.3 28.3 1.0 CZ A:ARG354 4.3 27.6 1.0 NZ A:LYS412 4.3 37.9 1.0 CG A:LYS412 4.4 34.0 1.0 NH2 A:ARG354 4.5 28.6 1.0 O A:SER340 4.5 28.9 1.0 N A:GLY341 4.7 27.8 1.0 C9 A:EPE1534 4.7 56.5 1.0 OH A:TYR414 4.8 37.8 1.0 C A:ARG342 4.9 28.4 1.0 N1 A:EPE1534 4.9 52.8 1.0 C A:SER340 5.0 26.9 1.0

Chlorine binding site 3 out of 4 in the Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase. within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1531 b:43.1 occ:1.00 O A:HOH2091 3.0 43.8 1.0 O A:HOH2236 3.2 34.5 1.0 N A:ASN385 3.2 25.7 1.0 CB A:ASN385 3.8 23.9 1.0 CA A:ASN385 3.9 25.8 1.0 CG A:LYS386 4.0 23.9 1.0 OG A:SER384 4.0 28.6 1.0 N A:LYS386 4.0 25.1 1.0 O A:HOH2237 4.1 39.0 1.0 C A:SER384 4.2 27.7 1.0 CA A:SER384 4.2 28.8 1.0 C A:ASN385 4.4 24.4 1.0 CG A:ASN385 4.4 24.1 1.0 ND2 A:ASN385 4.5 26.2 1.0 O A:HOH2234 4.6 39.9 1.0 CE A:LYS386 4.6 35.1 1.0 CD A:LYS386 4.7 32.1 1.0 CB A:SER384 4.7 29.9 1.0 CB A:LYS386 4.8 25.7 1.0 CA A:LYS386 5.0 26.0 1.0

Chlorine binding site 4 out of 4 in the Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase.


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase. within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1532 b:39.1 occ:1.00 NZ A:LYS187 3.1 45.5 1.0 N A:VAL146 3.4 36.5 1.0 O A:VAL146 3.5 35.1 1.0 CE A:LYS183 3.7 53.5 1.0 CA A:SER145 3.7 37.6 1.0 NZ A:LYS183 3.7 44.3 1.0 O A:HOH2055 3.8 41.9 1.0 CE A:LYS187 3.9 42.3 1.0 CD A:LYS183 3.9 49.2 1.0 C A:SER145 4.0 37.9 1.0 CB A:SER145 4.2 39.4 1.0 OG A:SER145 4.2 47.2 1.0 O A:PHE144 4.3 37.0 1.0 C A:VAL146 4.4 35.5 1.0 CA A:VAL146 4.4 34.8 1.0 CG2 A:VAL146 4.5 37.6 1.0 CG A:LYS183 4.8 40.1 1.0 N A:SER145 4.8 36.1 1.0 O A:HOH2085 4.9 49.6 1.0 C A:PHE144 5.0 35.9 1.0

C.J.Mccleverty, M.Hornsby, G.Spraggon, A.Kreusch. Crystal Structure of Human PUS10, A Novel Pseudouridine Synthase. J.Mol.Biol. V. 373 1243 2007.
ISSN: ISSN 0022-2836
PubMed: 17900615
DOI: 10.1016/J.JMB.2007.08.053