Chlorine in PDB 2vpq: Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp

All present enzymatic activity of Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp:
6.3.4.14;

The structure of Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp, PDB code: 2vpq was solved by I.Mochalkin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.1
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	78.247, 63.318, 105.142, 90.00, 103.82, 90.00
R / Rfree (%)	21.5 / 28.4

The structure of Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp also contains other interesting chemical elements:

Magnesium

(Mg)

4 atoms

The binding sites of Chlorine atom in the Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp (pdb code 2vpq). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp, PDB code: 2vpq:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1452 b:32.4 occ:1.00 NH1 A:ARG292 2.7 23.6 1.0 O A:HOH2270 2.8 27.6 1.0 N A:VAL295 3.2 20.6 1.0 CG2 A:VAL295 3.4 20.1 1.0 CA A:GLN294 3.6 20.8 1.0 CZ A:ARG292 3.6 24.7 1.0 NH2 A:ARG292 3.6 24.2 1.0 CG A:GLN294 3.8 22.1 1.0 CB A:GLN294 3.8 20.8 1.0 C A:GLN294 3.9 20.6 1.0 CB A:VAL295 4.1 20.3 1.0 CB A:TYR81 4.2 21.5 1.0 CA A:VAL295 4.2 20.4 1.0 CG A:TYR81 4.3 22.2 1.0 OE1 A:GLU296 4.3 20.2 1.0 CD2 A:TYR81 4.5 22.4 1.0 O A:ILE293 4.6 19.6 1.0 CD A:GLN294 4.6 23.0 1.0 NE2 A:GLN294 4.7 26.0 1.0 CD1 A:TYR81 4.8 22.9 1.0 N A:GLN294 4.9 20.5 1.0 NE A:ARG292 4.9 24.3 1.0 NH2 A:ARG338 4.9 21.9 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Biotin Carboxylase From S. Aureus Complexed with Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1453 b:36.8 occ:1.00 O B:HOH2247 2.9 46.9 1.0 NH1 B:ARG292 2.9 23.9 1.0 N B:VAL295 3.2 18.6 1.0 CG2 B:VAL295 3.3 18.1 1.0 CA B:GLN294 3.7 19.7 1.0 CZ B:ARG292 3.7 23.1 1.0 CG B:GLN294 3.7 20.0 1.0 NH2 B:ARG292 3.8 22.6 1.0 C B:GLN294 3.9 19.1 1.0 CB B:GLN294 3.9 20.0 1.0 CB B:VAL295 4.0 18.6 1.0 CG B:TYR81 4.1 22.8 1.0 CB B:TYR81 4.1 22.4 1.0 CA B:VAL295 4.1 18.6 1.0 O B:HOH2276 4.3 37.8 1.0 CD2 B:TYR81 4.4 23.6 1.0 CD1 B:TYR81 4.5 23.4 1.0 OE1 B:GLU296 4.5 17.7 1.0 O B:ILE293 4.7 20.0 1.0 CD B:GLN294 4.8 20.9 1.0 NE2 B:GLN294 4.8 20.4 1.0 NE B:ARG292 4.9 23.7 1.0 N B:GLN294 4.9 20.0 1.0

I.Mochalkin, J.R.Miller, A.Evdokimov, S.Lightle, C.Yan, C.K.Stover, G.L.Waldrop. Structural Evidence For Substrate-Induced Synergism and Half-Sites Reactivity in Biotin Carboxylase. Protein Sci. V. 17 1706 2008.
ISSN: ISSN 0961-8368
PubMed: 18725455
DOI: 10.1110/PS.035584.108