Chlorine in PDB 2wd0: Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2

The structure of Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2, PDB code: 2wd0 was solved by M.Sotomayor, W.Weihofen, R.Gaudet, D.P.Corey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.77 / 2.74
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	179.693, 179.693, 63.982, 90.00, 90.00, 90.00
R / Rfree (%)	21.941 / 24.865

The structure of Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2 also contains other interesting chemical elements:

Calcium	(Ca)	6 atoms
Sodium	(Na)	2 atoms

The binding sites of Chlorine atom in the Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2 (pdb code 2wd0). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2, PDB code: 2wd0:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl701 b:33.6 occ:1.00 CA A:CA502 2.6 19.8 1.0 OE1 A:GLU74 3.1 21.2 1.0 NH1 A:ARG73 3.2 27.7 1.0 O A:VAL103 3.3 20.0 1.0 N A:LEU139 3.3 23.3 1.0 N A:VAL103 3.5 19.8 1.0 CA A:GLY102 3.5 19.8 1.0 OE1 A:GLU22 3.6 21.5 1.0 C A:GLY102 3.8 19.8 1.0 CA A:ASP138 3.8 22.9 1.0 OD1 A:ASP138 3.9 22.8 1.0 C A:VAL103 3.9 20.2 1.0 CB A:LEU139 3.9 23.4 1.0 CD A:ARG73 4.0 24.3 1.0 CZ A:ARG73 4.0 26.9 1.0 CB A:ASP138 4.0 22.9 1.0 C A:ASP138 4.1 23.1 1.0 CA A:LEU139 4.2 23.4 1.0 NE A:ARG73 4.3 26.1 1.0 CA A:VAL103 4.3 19.8 1.0 CD A:GLU74 4.3 22.1 1.0 CG A:ASP138 4.3 22.8 1.0 CD2 A:LEU139 4.4 22.9 1.0 CG A:LEU139 4.7 23.6 1.0 OD1 A:ASP105 4.7 18.7 1.0 N A:ASN104 4.8 20.4 1.0 OE2 A:GLU74 4.8 22.1 1.0 O A:GLY102 4.8 20.0 1.0 CD A:GLU22 4.8 21.0 1.0 N A:GLY102 4.9 20.1 1.0 O A:LEU139 4.9 23.8 1.0 NH2 A:ARG73 4.9 27.3 1.0 CB A:ARG73 5.0 22.7 1.0

Chlorine binding site 2 out of 3 in the Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl702 b:52.8 occ:1.00 N A:PHE9 3.0 16.7 1.0 CD A:ARG94 3.5 32.1 1.0 CB A:PHE9 3.7 16.2 1.0 CA A:PHE8 3.7 17.2 1.0 CB A:PHE8 3.8 17.3 1.0 CD2 A:PHE9 3.8 14.9 1.0 CG A:ARG94 3.8 31.0 1.0 C A:PHE8 3.9 16.6 1.0 CG2 A:ILE92 3.9 30.5 1.0 CA A:PHE9 3.9 16.4 1.0 CG A:PHE9 4.2 15.1 1.0 O A:PHE9 4.4 16.1 1.0 CB A:ARG94 4.6 29.9 1.0 C A:PHE9 4.7 16.4 1.0 NH1 A:ARG94 4.7 33.4 1.0 NE A:ARG94 4.7 33.4 1.0 CE2 A:PHE9 4.8 14.4 1.0

Chlorine binding site 3 out of 3 in the Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Nonsyndromic Deafness (DFNB12) Associated Mutant D124G of Mouse Cadherin-23 EC1-2 within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl701 b:41.8 occ:1.00 CA C:CA501 2.7 26.4 1.0 O C:HOH2016 2.8 31.3 1.0 NH1 C:ARG73 3.1 28.3 1.0 O C:VAL103 3.2 21.2 1.0 N C:LEU139 3.3 26.4 1.0 N C:VAL103 3.3 20.5 1.0 OE1 C:GLU74 3.3 21.3 1.0 CA C:GLY102 3.4 20.4 1.0 C C:GLY102 3.6 20.3 1.0 OE1 C:GLU22 3.6 15.3 1.0 C C:VAL103 3.8 21.0 1.0 CA C:ASP138 3.8 26.7 1.0 CB C:LEU139 3.9 25.9 1.0 CB C:ASP138 3.9 26.9 1.0 OD1 C:ASP138 4.0 28.1 1.0 C C:ASP138 4.0 26.5 1.0 CZ C:ARG73 4.1 27.0 1.0 CA C:VAL103 4.1 20.6 1.0 CA C:LEU139 4.2 25.9 1.0 CD2 C:LEU139 4.2 25.5 1.0 CD C:ARG73 4.3 24.0 1.0 CG C:ASP138 4.4 27.4 1.0 CD C:GLU74 4.4 21.7 1.0 NE C:ARG73 4.5 25.7 1.0 O C:GLY102 4.6 20.2 1.0 CG C:LEU139 4.6 25.6 1.0 N C:ASN104 4.6 21.2 1.0 N C:GLY102 4.7 20.7 1.0 OD1 C:ASP105 4.7 22.1 1.0 OE2 C:GLU74 4.8 21.7 1.0 CD C:GLU22 4.8 15.4 1.0 O C:LEU139 4.8 26.2 1.0 NH2 C:ARG73 4.9 27.7 1.0 CB C:ARG73 5.0 21.4 1.0

M.Sotomayor, W.Weihofen, R.Gaudet, D.P.Corey. Structural Determinants of Cadherin-23 Function in Hearing and Deafness. Neuron V. 66 85 2010.
ISSN: ISSN 0896-6273
PubMed: 20399731
DOI: 10.1016/J.NEURON.2010.03.028