Chlorine in PDB 2wkr: Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant

The structure of Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant, PDB code: 2wkr was solved by Y.I.Wu, D.Frey, O.I.Lungu, A.Jaehrig, I.Schlichting, B.Kuhlman, K.M.Hahn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.60 / 2.20
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	112.390, 112.390, 69.200, 90.00, 90.00, 120.00
R / Rfree (%)	18.6 / 22.6

The structure of Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Chlorine atom in the Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant (pdb code 2wkr). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant, PDB code: 2wkr:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1725 b:26.6 occ:1.00 OG1 A:THR704 3.0 15.9 1.0 O A:HOH2199 3.2 31.3 1.0 CB A:ARG706 3.7 21.1 1.0 CB A:ASP661 3.8 16.4 1.0 CA A:ASP661 3.8 19.1 1.0 CD A:ARG706 3.8 37.4 1.0 CG2 A:THR704 3.8 19.1 1.0 CB A:THR704 3.9 19.3 1.0 N A:ASP661 4.0 16.8 1.0 CB A:SER701 4.0 16.7 1.0 CG A:ARG706 4.3 27.0 1.0 CG A:LEU660 4.4 20.9 1.0 OG A:SER701 4.4 15.8 1.0 C A:LEU660 4.5 16.6 1.0 CD2 A:LEU660 4.6 17.5 1.0 CB A:LEU660 4.7 17.6 1.0 O A:LEU660 4.9 17.1 1.0

Chlorine binding site 2 out of 2 in the Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1726 b:25.1 occ:1.00 OG A:SER626 3.0 11.3 1.0 NZ A:LYS659 3.2 16.5 1.0 CB A:SER626 3.5 13.7 1.0 O A:HOH2082 3.5 28.5 1.0 CE A:LYS659 3.6 15.9 1.0 CB A:SER629 3.8 16.1 1.0 CG2 A:VAL628 4.0 14.2 1.0 CD A:LYS659 4.0 14.3 1.0 O A:ALA556 4.4 15.8 1.0 N A:SER629 4.5 13.0 1.0 C A:VAL628 4.5 16.9 1.0 O A:HOH2084 4.5 33.7 1.0 O A:VAL628 4.6 19.3 1.0 CA A:SER629 4.7 17.2 1.0 O A:HOH2167 4.7 28.8 1.0 OG A:SER629 4.8 15.0 1.0 CA A:SER626 5.0 13.5 1.0

Y.I.Wu, D.Frey, O.I.Lungu, A.Jaehrig, I.Schlichting, B.Kuhlman, K.M.Hahn. A Genetically Encoded Photoactivatable Rac Controls the Motility of Living Cells. Nature V. 461 104 2009.
ISSN: ISSN 0028-0836
PubMed: 19693014
DOI: 10.1038/NATURE08241