Chlorine in PDB 2xgl: The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential

The structure of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential, PDB code: 2xgl was solved by F.Gerard, M.A.Brooks, H.Barreteau, T.Touze, M.Graille, A.Bouhss, D.Blanot, H.V.Tilbeurgh, D.Mengin-Lecreulx, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.24 / 2.70
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	52.000, 101.100, 43.520, 90.00, 90.00, 90.00
R / Rfree (%)	22.2 / 26.8

The structure of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential also contains other interesting chemical elements:

Cadmium	(Cd)	7 atoms
Sodium	(Na)	14 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 11;

Binding sites:

The binding sites of Chlorine atom in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential (pdb code 2xgl). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 11 binding sites of Chlorine where determined in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential, PDB code: 2xgl:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Chlorine binding site 1 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1121 b:56.3 occ:1.00 N A:TRP70 3.5 16.4 1.0 CD1 A:TRP70 3.8 14.4 1.0 CB A:TRP70 4.0 15.8 1.0 O A:TRP70 4.1 20.2 1.0 CA A:TRP70 4.2 16.1 1.0 CG A:TRP70 4.3 14.0 1.0 CA A:THR69 4.3 15.7 1.0 C A:THR69 4.3 15.3 1.0 C A:TRP70 4.6 18.6 1.0 CB A:THR69 4.6 17.4 1.0 NE1 A:TRP70 4.9 13.1 1.0

Chlorine binding site 2 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1125 b:46.4 occ:1.00 N A:GLU74 2.5 25.4 1.0 O A:LEU87 2.8 8.4 1.0 O A:GLU74 3.3 24.8 1.0 C A:ILE73 3.4 23.6 1.0 CA A:ILE73 3.4 21.6 1.0 CA A:GLU74 3.4 28.2 1.0 CB A:LEU87 3.5 9.9 1.0 C A:LEU87 3.6 8.1 1.0 CB A:GLU74 3.6 31.2 1.0 C A:GLU74 3.8 25.7 1.0 CG2 A:ILE73 3.8 17.8 1.0 CA A:LEU87 4.0 8.4 1.0 O A:LYS72 4.1 25.6 1.0 CG A:LEU87 4.1 12.9 1.0 CD2 A:LEU87 4.2 15.4 1.0 CB A:ILE73 4.2 20.0 1.0 CD A:PRO89 4.3 8.7 1.0 N A:LEU87 4.3 7.9 1.0 NA A:NA1131 4.4 33.7 1.0 CG A:GLU74 4.5 41.3 1.0 CG A:PRO89 4.5 9.9 1.0 O A:ILE73 4.5 23.9 1.0 N A:ILE73 4.5 23.7 1.0 C A:LYS72 4.7 25.5 1.0 N A:VAL88 4.7 7.4 1.0 CG1 A:ILE73 5.0 17.7 1.0

Chlorine binding site 3 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1126 b:24.6 occ:1.00 CD B:CD1116 2.4 27.8 1.0 NZ A:LYS84 3.1 9.4 1.0 CE A:LYS84 3.4 10.9 1.0 NA B:NA1126 3.5 54.1 1.0 CB B:LYS84 3.6 11.2 1.0 OD2 A:ASP82 3.6 12.4 1.0 CG A:LYS84 3.6 11.0 1.0 OD1 A:ASP82 3.7 20.3 1.0 CG A:ASP82 4.0 13.3 1.0 OE1 B:GLU109 4.0 17.7 1.0 OE2 B:GLU109 4.0 15.5 1.0 ND1 B:HIS106 4.1 16.1 1.0 CD A:LYS84 4.1 10.4 1.0 O A:ASP82 4.2 11.7 1.0 CG2 B:THR85 4.2 10.7 1.0 CD B:GLU109 4.3 13.9 1.0 CG B:LYS84 4.4 11.7 1.0 O B:LYS84 4.4 10.4 1.0 CE1 B:HIS106 4.6 13.2 1.0 CG B:HIS106 4.6 11.8 1.0 C B:LYS84 4.6 10.3 1.0 CA B:LYS84 4.7 10.9 1.0 CB B:HIS106 4.9 10.0 1.0 CD B:LYS84 5.0 11.2 1.0

Chlorine binding site 4 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1128 b:49.8 occ:1.00 CD A:CD1119 2.4 48.2 1.0 OH A:TYR102 3.1 27.6 1.0 CE A:MET100 3.4 22.1 1.0 CG A:MET100 3.6 20.0 1.0 SD A:MET100 3.7 25.1 1.0 CZ A:TYR102 4.0 23.4 1.0 CE1 A:TYR102 4.3 21.9 1.0 OE2 A:GLU71 4.6 40.0 1.0 CB A:MET100 4.8 17.8 1.0

Chlorine binding site 5 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1129 b:45.2 occ:1.00 NA A:NA1122 3.0 45.6 1.0 NZ A:LYS84 3.1 9.4 1.0 OE1 A:GLU108 3.1 20.5 1.0 O A:GLU81 3.2 12.6 1.0 CE A:LYS84 3.4 10.9 1.0 CD A:LYS84 3.6 10.4 1.0 O A:GLU83 3.9 11.3 1.0 O A:ASP82 3.9 11.7 1.0 C A:ASP82 4.0 11.9 1.0 CA A:ASP82 4.0 11.9 1.0 CD A:GLU108 4.1 17.9 1.0 C A:GLU81 4.2 13.1 1.0 CB A:ALA30 4.4 11.7 1.0 N A:ASP82 4.6 12.3 1.0 OE2 A:GLU108 4.6 21.3 1.0 OE2 B:GLU109 4.6 15.5 1.0 C A:GLU83 4.6 11.8 1.0 N A:GLU83 4.7 12.6 1.0 CG A:LYS84 4.8 11.0 1.0

Chlorine binding site 6 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1130 b:43.4 occ:1.00 O A:GLU71 2.8 22.3 1.0 NE A:ARG40 3.0 16.3 1.0 O A:PRO89 3.0 8.0 1.0 NH2 A:ARG40 3.4 17.7 1.0 CE3 A:TRP70 3.5 7.8 1.0 N A:GLU71 3.5 20.2 1.0 CG1 A:ILE73 3.6 17.7 1.0 CB A:TRP70 3.6 15.8 1.0 CG1 A:VAL88 3.6 5.9 1.0 CZ A:ARG40 3.6 19.0 1.0 CD A:PRO89 3.6 8.7 1.0 C A:GLU71 3.8 23.7 1.0 N A:PRO89 3.9 7.8 1.0 CG A:ARG40 4.0 10.6 1.0 CD A:ARG40 4.0 13.3 1.0 C A:PRO89 4.0 8.3 1.0 CA A:TRP70 4.1 16.1 1.0 CD2 A:TRP70 4.1 10.3 1.0 CD1 A:ILE73 4.2 15.0 1.0 CG A:TRP70 4.2 14.0 1.0 C A:VAL88 4.2 7.0 1.0 CA A:GLU71 4.3 23.0 1.0 C A:TRP70 4.3 18.6 1.0 CA A:VAL88 4.3 6.8 1.0 CZ3 A:TRP70 4.5 7.5 1.0 CA A:PRO89 4.5 8.2 1.0 CB A:VAL88 4.6 6.4 1.0 N A:ILE73 4.6 23.7 1.0 CG A:PRO89 4.7 9.9 1.0 CB A:GLU71 4.9 23.5 1.0 C A:LYS72 4.9 25.5 1.0 N A:LYS72 4.9 26.6 1.0 CB A:ILE73 4.9 20.0 1.0 NH1 A:ARG40 4.9 22.9 1.0 O A:VAL88 5.0 6.7 1.0

Chlorine binding site 7 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1118 b:27.6 occ:1.00 CD A:CD1116 2.4 29.8 1.0 NZ B:LYS84 3.1 10.3 1.0 ND1 A:HIS106 3.3 17.7 1.0 CE B:LYS84 3.5 11.5 1.0 OE2 A:GLU109 3.5 23.6 1.0 CB A:LYS84 3.6 10.9 1.0 OD2 B:ASP82 3.6 13.6 1.0 OD1 B:ASP82 3.7 22.5 1.0 CG B:LYS84 3.8 11.7 1.0 CG B:ASP82 4.0 15.1 1.0 OE1 A:GLU109 4.1 20.0 1.0 CG2 A:THR85 4.1 11.1 1.0 CE1 A:HIS106 4.1 14.2 1.0 CD A:GLU109 4.2 18.3 1.0 O A:LYS84 4.2 10.3 1.0 CD B:LYS84 4.3 11.2 1.0 O B:ASP82 4.3 12.9 1.0 CG A:HIS106 4.4 12.4 1.0 CG A:LYS84 4.4 11.0 1.0 C A:LYS84 4.5 10.0 1.0 CB A:HIS106 4.6 10.9 1.0 CA A:LYS84 4.7 10.4 1.0

Chlorine binding site 8 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 8 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1119 b:39.8 occ:1.00 N B:TYR114 3.1 11.1 1.0 O B:TYR114 3.4 15.0 1.0 CA B:GLU113 3.9 10.7 1.0 CA B:TYR114 3.9 11.3 1.0 C B:GLU113 3.9 10.2 1.0 CB B:TYR114 4.0 10.7 1.0 CB B:GLU113 4.1 12.2 1.0 C B:TYR114 4.1 13.3 1.0 CD2 B:TYR114 4.3 7.2 1.0 NH2 B:ARG50 4.4 24.8 1.0 OE2 B:GLU113 4.6 24.3 1.0 CG B:TYR114 4.7 7.6 1.0 CG B:GLU113 4.7 15.6 1.0 CD B:GLU113 4.7 20.8 1.0

Chlorine binding site 9 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 9 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1121 b:73.3 occ:1.00 OE1 B:GLU93 3.5 29.3 1.0 CD B:GLU93 3.8 24.4 1.0 CD B:LYS67 4.2 29.2 1.0 OE2 B:GLU93 4.3 32.3 1.0 CG B:GLU93 4.3 17.6 1.0 CB B:GLU93 4.7 12.8 1.0 CB B:LYS67 4.8 14.7 1.0

Chlorine binding site 10 out of 11 in the The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 10 of The X-Ray Structure of the Escherichia Coli Colicin M Immunity Protein Demonstrates the Presence of A Disulphide Bridge, Which Is Functionally Essential within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1122 b:50.1 occ:1.00 CD B:CD1117 2.5 45.3 1.0 OH B:TYR102 3.4 25.5 1.0 CG B:MET100 4.1 19.6 1.0 CE B:MET100 4.3 23.4 1.0 CZ B:TYR102 4.3 23.2 1.0 SD B:MET100 4.3 25.4 1.0 CE1 B:TYR102 4.4 21.7 1.0 OE2 B:GLU71 4.8 36.9 1.0

F.Gerard, M.A.Brooks, H.Barreteau, T.Touze, M.Graille, A.Bouhss, D.Blanot, H.V.Tilbeurgh, D.Mengin-Lecreulx. X-Ray Structure and Site-Directed Mutagenesis Analysis of the Escherichia Coli Colicin M Immunity Protein. J.Bacteriol. V. 193 205 2011.
ISSN: ISSN 0021-9193
PubMed: 21037007
DOI: 10.1128/JB.01119-10