Chlorine in PDB 2zag: Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus

The structure of Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus, PDB code: 2zag was solved by N.Maita, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.00
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	137.402, 266.397, 73.982, 90.00, 90.00, 90.00
R / Rfree (%)	22 / 27.2

The structure of Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus also contains other interesting chemical elements:

Calcium

(Ca)

4 atoms

The binding sites of Chlorine atom in the Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus (pdb code 2zag). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus, PDB code: 2zag:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl5 b:49.0 occ:1.00 NH1 A:ARG866 2.8 25.6 1.0 NH2 A:ARG762 3.5 30.6 1.0 CD A:ARG866 3.7 23.8 1.0 CG A:PRO820 3.7 35.3 1.0 CZ A:PHE895 3.9 29.7 1.0 CA A:SER559 3.9 30.3 1.0 CB A:SER559 3.9 31.5 1.0 CZ A:ARG866 3.9 24.8 1.0 OG A:SER559 4.0 34.8 1.0 CE1 A:PHE895 4.2 30.2 1.0 NE A:ARG866 4.3 24.4 1.0 O A:SER559 4.5 31.7 1.0 CZ A:ARG762 4.5 28.3 1.0 CD A:PRO820 4.6 34.4 1.0 NH1 A:ARG762 4.6 25.9 1.0 CG2 A:VAL893 4.7 30.2 1.0 C A:SER559 4.7 30.8 1.0 N A:SER559 4.9 30.1 1.0 CE2 A:PHE895 4.9 32.6 1.0 CD1 A:ILE950 5.0 36.2 1.0

Chlorine binding site 2 out of 4 in the Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl6 b:69.9 occ:1.00 NH1 B:ARG866 3.0 36.1 1.0 NH2 B:ARG762 3.4 27.6 1.0 CZ B:PHE895 3.8 41.2 1.0 CD B:PRO820 4.0 49.4 1.0 CD B:ARG866 4.0 36.2 1.0 CA B:SER559 4.0 38.3 1.0 OG B:SER559 4.1 37.6 1.0 CG B:PRO820 4.1 49.5 1.0 CB B:SER559 4.1 37.9 1.0 CZ B:ARG866 4.2 36.9 1.0 CE1 B:PHE895 4.4 41.2 1.0 NE B:ARG866 4.6 36.6 1.0 CB B:PRO820 4.6 49.3 1.0 CZ B:ARG762 4.6 30.4 1.0 O B:SER559 4.6 40.0 1.0 CE2 B:PHE895 4.7 41.1 1.0 C B:SER559 4.8 39.0 1.0 CG2 B:VAL893 4.9 41.2 1.0 NH1 B:ARG762 4.9 31.7 1.0 CD1 B:ILE950 5.0 47.9 1.0

Chlorine binding site 3 out of 4 in the Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl7 b:79.2 occ:1.00 NH1 C:ARG866 3.1 50.4 1.0 CZ C:PHE895 3.6 64.8 1.0 NH2 C:ARG762 3.7 52.9 1.0 CG C:PRO820 3.7 54.8 1.0 CD C:ARG866 3.9 53.4 1.0 CA C:SER559 4.1 44.9 1.0 OG C:SER559 4.2 46.1 1.0 CB C:SER559 4.2 45.9 1.0 CZ C:ARG866 4.3 50.0 1.0 NH1 C:ARG762 4.3 52.5 1.0 CE1 C:PHE895 4.3 65.0 1.0 CD C:PRO820 4.4 55.2 1.0 CZ C:ARG762 4.4 52.7 1.0 CE2 C:PHE895 4.5 65.0 1.0 NE C:ARG866 4.6 50.5 1.0 O C:SER559 4.7 44.9 1.0 CD1 C:ILE950 4.9 61.9 1.0 C C:SER559 4.9 45.0 1.0

Chlorine binding site 4 out of 4 in the Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of the Semet-Substituted Soluble Domain of STT3 From P. Furiosus within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl8 b:48.5 occ:1.00 NH1 D:ARG866 2.8 15.6 1.0 NH2 D:ARG762 3.4 27.6 1.0 CZ D:PHE895 3.5 18.1 1.0 CD D:ARG866 3.7 18.5 1.0 CE1 D:PHE895 3.9 19.8 1.0 CZ D:ARG866 3.9 17.4 1.0 CA D:SER559 4.0 27.2 1.0 CB D:SER559 4.0 26.6 1.0 CD D:PRO820 4.2 33.6 1.0 CG D:PRO820 4.3 31.4 1.0 NE D:ARG866 4.3 15.4 1.0 OG D:SER559 4.4 31.6 1.0 CZ D:ARG762 4.5 26.9 1.0 CE2 D:PHE895 4.5 18.8 1.0 O D:SER559 4.6 28.1 1.0 CB D:PRO820 4.7 32.4 1.0 NH1 D:ARG762 4.7 31.0 1.0 CG2 D:VAL893 4.8 20.7 1.0 C D:SER559 4.8 28.3 1.0 N D:SER559 5.0 27.0 1.0

M.Igura, N.Maita, J.Kamishikiryo, M.Yamada, T.Obita, K.Maenaka, D.Kohda. Structure-Guided Identification of A New Catalytic Motif of Oligosaccharyltransferase Embo J. V. 27 234 2008.
ISSN: ISSN 0261-4189
PubMed: 18046457
DOI: 10.1038/SJ.EMBOJ.7601940