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Chlorine in PDB 2zak: Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation

Enzymatic activity of Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation

All present enzymatic activity of Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation:
3.4.19.5; 3.5.1.1;

Protein crystallography data

The structure of Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation, PDB code: 2zak was solved by K.Michalska, A.Hernandez-Santoyo, M.Jaskolski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 2.01
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 51.350, 77.780, 147.930, 90.00, 90.00, 90.00
R / Rfree (%) 19.7 / 25.3

Other elements in 2zak:

The structure of Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation (pdb code 2zak). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation, PDB code: 2zak:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in 2zak

Go back to Chlorine Binding Sites List in 2zak
Chlorine binding site 1 out of 2 in the Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl323

b:33.5
occ:1.00
N A:ILE310 3.0 21.8 1.0
N A:TYR311 3.3 20.2 1.0
CA A:GLY309 3.7 23.8 1.0
C A:GLY309 3.9 23.2 1.0
CA A:ILE310 3.9 21.2 1.0
O A:TYR311 4.0 20.0 1.0
CD2 A:TYR311 4.0 21.8 1.0
C A:ILE310 4.1 20.8 1.0
CA A:TYR311 4.2 21.4 1.0
CB A:TYR311 4.2 21.5 1.0
CB A:ILE310 4.3 20.8 1.0
C A:TYR311 4.4 21.7 1.0
CG A:TYR311 4.5 22.8 1.0
CE2 A:TYR311 5.0 24.5 1.0

Chlorine binding site 2 out of 2 in 2zak

Go back to Chlorine Binding Sites List in 2zak
Chlorine binding site 2 out of 2 in the Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Orthorhombic Crystal Structure of Precursor E. Coli Isoaspartyl Peptidase/L-Asparaginase (Ecaiii) with Active-Site T179A Mutation within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl323

b:44.9
occ:1.00
N B:ILE310 3.2 21.8 1.0
N B:TYR311 3.2 22.6 1.0
O B:TYR311 3.9 24.8 1.0
CA B:ILE310 4.0 22.0 1.0
CD2 B:TYR311 4.0 22.0 1.0
CA B:TYR311 4.0 23.7 1.0
C B:ILE310 4.0 22.4 1.0
CB B:TYR311 4.1 23.3 1.0
C B:GLY309 4.2 22.0 1.0
C B:TYR311 4.2 24.7 1.0
CB B:ILE310 4.2 22.0 1.0
CA B:GLY309 4.2 21.9 1.0
CG B:TYR311 4.5 22.6 1.0
CG1 B:ILE310 5.0 22.5 1.0
CE2 B:TYR311 5.0 23.5 1.0

Reference:

K.Michalska, D.Borek, A.Hernandez-Santoyo, M.Jaskolski. Crystal Packing of Plant-Type L-Asparaginase From Escherichia Coli Acta Crystallogr.,Sect.D V. 64 309 2008.
ISSN: ISSN 0907-4449
PubMed: 18323626
DOI: 10.1107/S0907444907068072
Page generated: Sat Dec 12 09:30:13 2020

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