Chlorine in PDB 3ajv: Splicing Endonuclease From Aeropyrum Pernix

All present enzymatic activity of Splicing Endonuclease From Aeropyrum Pernix:
3.1.27.9;

The structure of Splicing Endonuclease From Aeropyrum Pernix, PDB code: 3ajv was solved by S.Yoshinari, Y.Watanabe, M.Okuda, T.Shiba, K.D.Inaoka, G.Kurisu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.70
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	95.334, 95.334, 253.819, 90.00, 90.00, 120.00
R / Rfree (%)	21.8 / 22.9

The binding sites of Chlorine atom in the Splicing Endonuclease From Aeropyrum Pernix (pdb code 3ajv). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Splicing Endonuclease From Aeropyrum Pernix, PDB code: 3ajv:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Splicing Endonuclease From Aeropyrum Pernix


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Splicing Endonuclease From Aeropyrum Pernix within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl191 b:26.2 occ:1.00 OG B:SER57 2.9 11.8 1.0 O C:HOH190 2.9 8.0 1.0 O B:HOH289 2.9 20.4 1.0 NE C:ARG154 3.2 15.4 1.0 CG1 B:ILE59 3.5 13.4 0.8 CD1 B:ILE59 3.6 14.0 0.8 CB B:SER57 3.6 14.7 1.0 NH2 C:ARG154 3.9 9.2 1.0 CG B:GLU60 3.9 14.8 1.0 CZ C:ARG154 4.0 9.5 1.0 CD C:ARG154 4.1 14.6 1.0 CG2 C:THR155 4.1 11.5 1.0 CD B:GLU60 4.1 16.4 1.0 CG C:ARG154 4.2 13.9 1.0 CE1 B:TYR102 4.4 12.9 1.0 OE1 B:GLU60 4.4 16.8 1.0 OE2 B:GLU60 4.5 17.6 1.0 CD1 B:TYR102 4.5 9.1 1.0 CB B:ILE59 4.6 10.1 0.2 CD1 B:ILE59 4.9 8.4 0.2 N B:GLU60 4.9 9.7 1.0 CB B:GLU60 4.9 13.6 1.0 CG2 B:ILE59 5.0 9.4 0.2 CA B:SER57 5.0 13.7 1.0 CB B:ILE59 5.0 10.7 0.8

Chlorine binding site 2 out of 2 in the Splicing Endonuclease From Aeropyrum Pernix


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Splicing Endonuclease From Aeropyrum Pernix within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl190 b:26.9 occ:1.00 OG D:SER57 2.9 12.3 1.0 O A:HOH198 2.9 3.0 1.0 NE A:ARG154 3.1 15.0 1.0 O D:HOH219 3.3 20.7 1.0 CG1 D:ILE59 3.4 14.9 1.0 CD1 D:ILE59 3.5 15.8 1.0 CB D:SER57 3.6 14.1 1.0 CD A:ARG154 3.9 13.1 1.0 NH2 A:ARG154 4.0 10.0 1.0 CG D:GLU60 4.0 14.1 1.0 CZ A:ARG154 4.0 10.3 1.0 CG A:ARG154 4.1 12.7 1.0 CG2 A:THR155 4.1 13.1 1.0 CD D:GLU60 4.2 16.7 1.0 CE1 D:TYR102 4.4 12.8 1.0 CD1 D:TYR102 4.5 7.7 1.0 OE1 D:GLU60 4.5 15.9 1.0 OE2 D:GLU60 4.6 15.5 1.0 N D:GLU60 4.9 10.9 1.0 CB D:ILE59 4.9 12.7 1.0 CB D:GLU60 5.0 14.9 1.0

M.Okuda, T.Shiba, D.K.Inaoka, K.Kita, G.Kurisu, S.Mineki, S.Harada, Y.Watanabe, S.Yoshinari. A Conserved Lysine Residue in the Crenarchaea-Specific Loop Is Important For the Crenarchaeal Splicing Endonuclease Activity. J.Mol.Biol. V. 405 92 2011.
ISSN: ISSN 0022-2836
PubMed: 21050862
DOI: 10.1016/J.JMB.2010.10.050