Chlorine in PDB 3c4x: Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A

All present enzymatic activity of Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A:
2.7.11.14;

The structure of Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A, PDB code: 3c4x was solved by P.Singh, J.J.G.Tesmer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.75 / 2.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.116, 95.122, 234.823, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / n/a

The structure of Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Chlorine atom in the Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A (pdb code 3c4x). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A, PDB code: 3c4x:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl565 b:55.7 occ:1.00 NE2 B:GLN173 3.4 39.5 1.0 N A:LEU166 3.5 36.0 1.0 O A:HOH613 3.7 41.4 1.0 NH1 B:ARG170 3.8 39.3 1.0 CB A:SER165 3.8 37.3 1.0 CG A:LEU166 3.9 34.2 1.0 CA A:SER165 4.1 37.1 1.0 CB A:LEU166 4.1 35.7 1.0 CD1 A:LEU166 4.2 33.2 1.0 C A:SER165 4.3 36.6 1.0 CD B:GLN173 4.3 37.1 1.0 OE1 B:GLN173 4.4 41.5 1.0 CA A:LEU166 4.4 36.1 1.0 CD1 A:TYR167 4.4 32.6 1.0 CE1 A:TYR167 4.4 33.1 1.0 OG A:SER165 4.7 37.8 1.0 CZ B:ARG170 4.8 37.9 1.0 N A:TYR167 4.9 35.9 1.0 CE1 A:HIS79 5.0 54.0 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of G Protein Coupled Receptor Kinase 1 Bound to Atp and Magnesium Chloride at 2.9A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl566 b:41.8 occ:1.00 N A:GLN287 3.2 45.6 1.0 CD A:ARG290 3.5 43.4 1.0 CA A:PHE286 3.8 45.7 1.0 NH1 A:ARG290 3.8 45.6 1.0 CG A:GLN287 3.9 48.3 1.0 C A:PHE286 4.0 45.5 1.0 CB A:GLN287 4.0 46.3 1.0 CA A:GLN287 4.1 45.9 1.0 CB A:PHE286 4.3 45.6 1.0 CB A:ARG290 4.3 42.2 1.0 NE A:ARG290 4.5 44.9 1.0 CD2 A:PHE286 4.5 42.4 1.0 O A:GLY285 4.5 48.5 1.0 CG A:ARG290 4.5 43.3 1.0 CZ A:ARG290 4.6 44.9 1.0 O A:GLN287 4.6 44.5 1.0 CG2 A:VAL279 4.8 54.4 1.0 CG A:PHE286 4.8 43.7 1.0 C A:GLN287 4.9 45.5 1.0 N A:PHE286 5.0 46.1 1.0

P.Singh, B.Wang, T.Maeda, K.Palczewski, J.J.Tesmer. Structures of Rhodopsin Kinase in Different Ligand States Reveal Key Elements Involved in G Protein-Coupled Receptor Kinase Activation. J.Biol.Chem. V. 283 14053 2008.
ISSN: ISSN 0021-9258
PubMed: 18339619
DOI: 10.1074/JBC.M708974200