Chlorine in PDB 3cc7: Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U

The structure of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U, PDB code: 3cc7 was solved by G.Blaha, G.Gurel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.83 / 2.70
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	212.832, 299.896, 576.013, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 22.6

The structure of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U also contains other interesting chemical elements:

Strontium	(Sr)	108 atoms
Magnesium	(Mg)	93 atoms
Potassium	(K)	2 atoms
Cadmium	(Cd)	5 atoms
Sodium	(Na)	75 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 22;

Binding sites:

The binding sites of Chlorine atom in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U (pdb code 3cc7). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 22 binding sites of Chlorine where determined in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U, PDB code: 3cc7:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Chlorine binding site 1 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8803 b:50.8 occ:1.00 N2 0:G1676 3.3 39.5 1.0 O 0:HOH9444 3.4 30.4 1.0 O 0:HOH5871 3.5 59.3 1.0 CE 2:LYS2 3.6 33.8 1.0 C1' 0:G1452 3.8 41.1 1.0 O 0:HOH3600 3.8 50.4 1.0 O2' 0:G1452 3.9 41.0 1.0 O 0:HOH7634 4.0 51.6 1.0 N3 0:A1486 4.2 36.3 1.0 C2 0:A1486 4.2 34.5 1.0 C2 0:G1676 4.4 40.1 1.0 O4' 0:G1452 4.4 41.5 1.0 C2' 0:G1452 4.4 40.2 1.0 N3 0:G1676 4.5 41.9 1.0 NZ 2:LYS2 4.5 34.0 1.0 CD 2:LYS2 4.6 33.9 1.0 O 2:HOH3526 4.6 63.4 1.0 N9 0:G1452 4.7 43.2 1.0 O2 0:C1451 4.7 39.5 1.0 N3 0:G1452 4.8 43.3 1.0

Chlorine binding site 2 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8805 b:58.8 occ:1.00 O 0:HOH4590 3.2 32.5 1.0 N2 0:G201 3.2 43.4 1.0 O 0:HOH4966 3.3 63.0 1.0 O 0:HOH3220 3.5 39.5 1.0 C5 0:C197 3.6 44.7 1.0 C1' 0:U202 3.9 45.4 1.0 C5' 0:G229 3.9 43.3 1.0 O4' 0:U202 3.9 46.2 1.0 O3' 0:G229 4.1 40.1 1.0 C2 0:G201 4.2 44.1 1.0 C6 0:C197 4.3 45.5 1.0 C4' 0:G229 4.3 41.8 1.0 N3 0:G201 4.3 45.4 1.0 O2' 0:U202 4.5 46.9 1.0 N1 0:U202 4.6 44.8 1.0 O2 0:U202 4.6 45.9 1.0 C4 0:C197 4.7 45.2 1.0 OP1 0:C230 4.7 41.6 1.0 N4 0:C197 4.7 45.4 1.0 O2 0:C195 4.8 38.7 1.0 C3' 0:G229 4.8 41.0 1.0 O 0:HOH9778 4.9 41.5 1.0 N1 0:A199 4.9 49.6 1.0 C2 0:U202 4.9 43.7 1.0 C2' 0:U202 4.9 45.2 1.0 C4' 0:U202 5.0 46.0 1.0

Chlorine binding site 3 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8811 b:58.9 occ:1.00 N Q:HIS53 2.9 39.6 1.0 O2' 0:C2388 3.2 28.4 1.0 O Q:HOH1479 3.3 26.4 1.0 CA Q:PHE52 3.5 41.5 1.0 CB Q:PHE52 3.5 40.6 1.0 C Q:PHE52 3.7 41.1 1.0 CE2 Q:PHE56 3.7 31.5 1.0 CB Q:HIS53 3.8 37.7 1.0 CD2 Q:PHE56 3.8 31.1 1.0 O 0:HOH3617 3.8 44.9 1.0 CA Q:HIS53 3.9 37.2 1.0 CG2 Q:ILE84 4.0 40.5 1.0 O4' 0:C2388 4.1 32.0 1.0 C4' 0:C2388 4.1 31.1 1.0 C1' 0:C2388 4.1 32.4 1.0 C2' 0:C2388 4.2 31.9 1.0 CG1 Q:ILE84 4.5 42.1 1.0 O 0:HOH4445 4.5 41.0 1.0 C3' 0:C2388 4.7 32.1 1.0 O 0:HOH3184 4.7 50.0 1.0 CB Q:ILE84 4.7 40.6 1.0 O Q:PHE52 4.9 41.7 1.0 CD1 Q:ILE84 4.9 45.7 1.0 CG Q:PHE52 4.9 41.6 1.0 N Q:PHE52 4.9 42.8 1.0 O3' 0:C2388 5.0 32.5 1.0

Chlorine binding site 4 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8812 b:40.0 occ:1.00 NZ K:LYS14 3.1 28.9 1.0 O 0:HOH5155 3.1 27.0 1.0 OG K:SER33 3.3 49.0 1.0 N2 0:G2582 3.4 37.1 1.0 C2 0:A2596 3.4 40.6 1.0 N3 0:A2596 3.6 40.5 1.0 O K:HOH7499 3.8 42.0 1.0 CG K:PRO45 3.8 37.2 1.0 O K:ILE32 3.9 38.8 1.0 CB K:SER33 3.9 43.5 1.0 CD K:LYS14 4.0 28.7 1.0 C1' 0:U2597 4.0 38.4 1.0 CA K:SER33 4.0 41.8 1.0 CE K:LYS14 4.0 26.9 1.0 CB K:PRO45 4.1 37.5 1.0 O2 0:U2597 4.3 37.8 1.0 O2' 0:U2597 4.4 42.8 1.0 N1 0:A2596 4.5 41.1 1.0 N3 0:A2583 4.6 38.0 1.0 O4' 0:U2597 4.7 39.6 1.0 C2 0:G2582 4.7 37.5 1.0 CG K:LYS14 4.8 29.2 1.0 N1 0:U2597 4.8 37.2 1.0 C2' 0:U2597 4.8 39.4 1.0 C K:ILE32 4.8 40.1 1.0 N K:VAL34 4.9 41.1 1.0 C2 0:U2597 4.9 36.1 1.0 C1' 0:A2583 4.9 32.5 1.0 N K:SER33 4.9 40.2 1.0 C4 0:A2596 5.0 41.0 1.0

Chlorine binding site 5 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8813 b:44.2 occ:1.00 O 0:HOH4714 2.4 0.2 1.0 N1 0:G1329 3.0 27.3 1.0 N2 0:G1329 3.1 24.8 1.0 O 0:HOH5009 3.4 60.0 1.0 O 0:HOH3672 3.4 34.6 1.0 N6 0:A1328 3.5 22.0 1.0 C2 0:G1329 3.6 27.0 1.0 O 0:HOH9826 3.7 47.7 1.0 O 0:HOH4890 3.8 78.0 1.0 N2 0:G1299 3.8 28.7 1.0 O2' 0:G1300 3.8 25.1 1.0 C1' 0:G1300 4.1 28.9 1.0 C6 0:G1329 4.1 28.6 1.0 N1 0:A1330 4.2 34.7 1.0 O4' 0:G1300 4.2 27.9 1.0 O6 0:G1329 4.2 35.8 1.0 C2' 0:G1300 4.6 28.9 1.0 C2 0:A1330 4.7 35.5 1.0 N3 0:G1299 4.7 30.1 1.0 C2 0:G1299 4.7 29.3 1.0 C4' 0:G1300 4.8 29.1 1.0 C6 0:A1328 4.8 20.0 1.0 N3 0:G1329 4.9 27.4 1.0

Chlorine binding site 6 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8814 b:48.6 occ:1.00 O2' 0:G644 3.0 28.0 1.0 O 0:HOH7775 3.0 36.7 1.0 ND1 L:HIS13 3.1 24.9 1.0 C2' 0:G644 3.5 25.9 1.0 N9 0:G644 3.7 21.7 1.0 C4 0:G644 3.8 21.9 1.0 CG L:GLN7 3.9 29.8 1.0 C8 0:G644 4.0 22.4 1.0 CE1 L:HIS13 4.0 24.3 1.0 C1' 0:G644 4.0 23.4 1.0 CG L:HIS13 4.0 25.4 1.0 CB L:HIS13 4.1 24.2 1.0 N3 0:G644 4.2 24.0 1.0 C5 0:G644 4.2 22.5 1.0 N7 0:G644 4.3 22.4 1.0 CG L:LYS4 4.4 34.2 1.0 O 0:HOH3219 4.5 62.1 1.0 CB L:GLN7 4.6 28.4 1.0 O L:HIS13 4.8 27.5 1.0 C2 0:G644 4.8 25.4 1.0 CD L:GLN7 4.9 33.4 1.0 CA L:LYS4 4.9 31.7 1.0 C6 0:G644 5.0 24.2 1.0 OE1 L:GLN7 5.0 34.8 1.0 C3' 0:G644 5.0 25.4 1.0

Chlorine binding site 7 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8815 b:58.0 occ:1.00 N6 0:A1598 3.0 42.9 1.0 O 0:HOH6849 3.4 50.7 1.0 N2 0:G1646 3.5 48.7 1.0 N6 0:A1597 3.5 41.6 1.0 C6 0:A1598 4.0 43.9 1.0 C1' 0:G1647 4.0 46.6 1.0 O4' 0:G1647 4.0 45.6 1.0 N7 0:A1597 4.1 41.4 1.0 C1' 0:G1540 4.4 50.7 1.0 C6 0:A1597 4.5 43.0 1.0 C2 0:G1646 4.5 48.8 1.0 N3 0:G1646 4.6 48.2 1.0 C5 0:A1597 4.6 43.2 1.0 C5 0:A1598 4.7 44.3 1.0 O4' 0:G1540 4.7 49.2 1.0 N1 0:A1598 4.8 44.1 1.0 O2' 0:U1596 4.8 51.4 1.0 N7 0:A1598 4.8 44.8 1.0 O2' 0:G1540 4.9 51.8 1.0 N9 0:G1647 4.9 49.1 1.0 O2' 0:G1647 4.9 43.0 1.0 N3 0:G1540 4.9 51.7 1.0

Chlorine binding site 8 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 8 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8816 b:54.2 occ:1.00 O2' 0:G1119 3.1 34.4 1.0 NZ J:LYS56 3.1 37.4 1.0 N4 0:C1243 3.4 28.9 1.0 C8 0:G1119 3.4 31.7 1.0 CD J:LYS56 3.6 35.9 1.0 NE2 J:GLN52 3.8 47.3 1.0 CE J:LYS56 3.8 35.8 1.0 C2' 0:G1119 3.9 34.8 1.0 N7 0:G1119 4.0 31.1 1.0 N9 0:G1119 4.2 30.9 1.0 OE1 J:GLN52 4.2 46.2 1.0 O 0:HOH6999 4.2 76.1 1.0 CD J:GLN52 4.4 47.5 1.0 C4 0:C1243 4.5 32.2 1.0 C1' 0:G1119 4.5 32.5 1.0 C5 0:C1243 4.7 33.3 1.0 N6 0:A1242 4.8 34.1 1.0 CG J:LYS56 4.9 34.2 1.0 O 0:HOH3480 4.9 32.8 1.0 NA 0:NA8502 5.0 55.1 1.0 C5 0:G1119 5.0 28.1 1.0

Chlorine binding site 9 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 9 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8817 b:50.6 occ:1.00 O Y:HOH8852 2.5 85.2 1.0 O2' 0:C594 2.8 40.2 1.0 O Y:HOH8833 3.1 52.8 1.0 O Y:HOH8891 3.5 38.5 1.0 NE Y:ARG115 3.5 44.5 1.0 O4' 0:U595 3.5 48.4 1.0 CG Y:ARG115 3.6 41.8 1.0 C5' 0:U595 3.7 42.9 1.0 C4' 0:U595 3.7 46.7 1.0 CD Y:ARG115 3.8 42.2 1.0 C2' 0:C594 3.9 41.7 1.0 NH2 Y:ARG122 3.9 44.5 1.0 O 0:HOH6590 4.4 68.2 1.0 O 0:HOH6336 4.5 85.5 1.0 CZ Y:ARG115 4.6 45.0 1.0 O2 0:C594 4.7 48.8 1.0 C1' 0:C594 4.7 43.9 1.0 C1' 0:U595 4.8 49.6 1.0 O5' 0:U595 4.8 40.1 1.0 NE2 Y:GLN119 4.9 33.0 1.0 NH2 Y:ARG115 4.9 45.9 1.0

Chlorine binding site 10 out of 22 in the Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 10 of Structure of Anisomycin Resistant 50S Ribosomal Subunit: 23S Rrna Mutation C2487U within 5.0Å range: probe atom residue distance (Å) B Occ 0:Cl8822 b:74.5 occ:1.00 N1 0:G1072 3.0 24.1 1.0 N2 0:G1072 3.3 23.5 1.0 O2' 0:G1087 3.3 39.6 1.0 C8 0:G1087 3.5 30.1 1.0 N7 0:A1291 3.5 34.7 1.0 O 0:HOH5387 3.6 45.0 1.0 C2 0:G1072 3.6 24.2 1.0 N7 0:G1087 3.9 31.5 1.0 C2' 0:G1087 3.9 34.6 1.0 C5 0:A1291 4.0 34.0 1.0 C6 0:G1072 4.0 26.3 1.0 N9 0:G1087 4.0 31.0 1.0 O6 0:G1072 4.0 30.1 1.0 C8 0:A1291 4.1 31.6 1.0 O 0:HOH9157 4.1 44.9 1.0 C6 0:A1291 4.3 35.8 1.0 N6 0:A1291 4.4 36.8 1.0 C1' 0:G1087 4.4 34.0 1.0 C5 0:G1087 4.5 32.1 1.0 OE2 W:GLU10 4.5 50.0 1.0 C4 0:G1087 4.6 31.5 1.0 O 0:HOH4074 4.6 65.0 1.0 C4 0:A1291 4.7 33.4 1.0 N9 0:A1291 4.7 31.4 1.0 N3 0:G1072 4.9 25.9 1.0

G.Blaha, G.Gurel, S.J.Schroeder, P.B.Moore, T.A.Steitz. Mutations Outside the Anisomycin-Binding Site Can Make Ribosomes Drug-Resistant. J.Mol.Biol. V. 379 505 2008.
ISSN: ISSN 0022-2836
PubMed: 18455733
DOI: 10.1016/J.JMB.2008.03.075