Chlorine in PDB 3dkh: L559A Mutant of Melanocarpus Albomyces Laccase

All present enzymatic activity of L559A Mutant of Melanocarpus Albomyces Laccase:
1.10.3.2;

The structure of L559A Mutant of Melanocarpus Albomyces Laccase, PDB code: 3dkh was solved by N.Hakulinen, J.Rouvinen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.82 / 2.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	173.510, 62.020, 125.670, 90.00, 99.92, 90.00
R / Rfree (%)	22.4 / 28.2

The structure of L559A Mutant of Melanocarpus Albomyces Laccase also contains other interesting chemical elements:

Copper

(Cu)

8 atoms

The binding sites of Chlorine atom in the L559A Mutant of Melanocarpus Albomyces Laccase (pdb code 3dkh). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the L559A Mutant of Melanocarpus Albomyces Laccase, PDB code: 3dkh:

Chlorine binding site 1 out of 1 in the L559A Mutant of Melanocarpus Albomyces Laccase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of L559A Mutant of Melanocarpus Albomyces Laccase within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl610 b:49.9 occ:1.00 O A:HOH1022 2.7 20.8 1.0 N A:GLY96 2.9 21.2 1.0 CU A:CU604 3.0 48.0 1.0 NE2 A:HIS93 3.3 19.0 1.0 CE1 A:HIS93 3.6 18.4 1.0 CA A:HIS95 3.6 22.1 1.0 CE1 A:HIS434 3.7 13.7 1.0 O A:TRP94 3.7 20.7 1.0 NE2 A:HIS434 3.7 13.4 1.0 C A:HIS95 3.8 21.5 1.0 CA A:GLY96 3.8 25.1 1.0 O A:ILE97 4.0 27.2 1.0 O A:LEU435 4.0 7.5 1.0 CA A:HIS436 4.0 8.9 1.0 N A:ILE97 4.1 27.3 1.0 N A:GLY437 4.1 10.6 1.0 C A:GLY96 4.1 26.4 1.0 C A:TRP94 4.5 20.5 1.0 N A:HIS95 4.5 21.9 1.0 CD2 A:HIS95 4.5 21.9 1.0 O A:HOH1154 4.6 28.9 1.0 C A:HIS436 4.6 9.9 1.0 CD2 A:HIS93 4.6 18.6 1.0 C A:ILE97 4.6 28.8 1.0 CG A:HIS95 4.6 22.2 1.0 CB A:HIS95 4.7 21.4 1.0 CG A:HIS436 4.8 4.0 1.0 CB A:HIS436 4.8 7.6 1.0 C A:LEU435 4.8 9.2 1.0 N A:HIS436 4.9 8.7 1.0 ND1 A:HIS93 4.9 20.0 1.0 O A:GLY96 4.9 27.7 1.0 O A:HIS95 5.0 21.1 1.0 ND1 A:HIS434 5.0 13.9 1.0 O A:HOH1035 5.0 22.8 1.0

M.Andberg, N.Hakulinen, S.Auer, M.Saloheimo, A.Koivula, J.Rouvinen, K.Kruus. Essential Role of the C-Terminus in Melanocarpus Albomyces Laccase For Enzyme Production, Catalytic Properties and Structure Febs J. V. 276 6285 2009.
ISSN: ISSN 1742-464X
PubMed: 19780817
DOI: 10.1111/J.1742-4658.2009.07336.X