Atomistry » Chlorine » PDB 3es1-3f5r » 3ezw
Atomistry »
  Chlorine »
    PDB 3es1-3f5r »
      3ezw »

Chlorine in PDB 3ezw: Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices

Enzymatic activity of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices

All present enzymatic activity of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices:
2.7.1.30;

Protein crystallography data

The structure of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices, PDB code: 3ezw was solved by M.J.Anderson, B.Delabarre, P.Dunten, A.T.Brunger, S.R.Quake, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.00
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 91.116, 114.260, 212.624, 90.00, 91.15, 90.00
R / Rfree (%) 16.7 / 22.5

Other elements in 3ezw:

The structure of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices (pdb code 3ezw). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 8 binding sites of Chlorine where determined in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices, PDB code: 3ezw:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Chlorine binding site 1 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 1 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl2648

b:23.5
occ:1.00
 O A:HOH9099 2.9 42.3 1.0
NE A:ARG177 3.3 22.1 1.0
N A:GLN226 3.3 29.9 1.0
CG2 A:VAL178 3.6 20.5 1.0
CG A:GLN226 3.8 32.2 1.0
CA A:GLY225 3.8 32.5 1.0
O A:GLN226 3.9 27.2 1.0
CD A:ARG177 3.9 22.5 1.0
CB A:ARG177 3.9 23.2 1.0
C A:GLY225 4.1 30.9 1.0
CB A:GLN226 4.1 31.2 1.0
CA A:GLN226 4.1 29.0 1.0
CZ A:ARG177 4.3 23.7 1.0
CG2 A:THR174 4.3 23.8 1.0
CB A:THR174 4.3 22.5 1.0
NH2 A:ARG177 4.3 25.7 1.0
C A:GLN226 4.5 27.4 1.0
CG A:ARG177 4.5 23.6 1.0
CD A:GLN226 4.8 30.2 1.0
CB A:VAL178 5.0 22.4 1.0
O A:TYR224 5.0 31.0 1.0

Chlorine binding site 2 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 2 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl2649

b:33.4
occ:1.00
 O B:HOH2881 3.1 51.3 1.0
N B:GLN226 3.1 29.9 1.0
NE B:ARG177 3.1 28.0 1.0
O B:GLN226 3.3 30.2 1.0
NH2 B:ARG177 3.5 25.1 1.0
CA B:GLY225 3.6 28.7 1.0
CZ B:ARG177 3.8 27.7 1.0
CG2 B:VAL178 3.8 22.6 1.0
C B:GLY225 3.9 29.3 1.0
O B:HOH2828 4.1 37.4 1.0
CG2 B:THR174 4.1 21.7 1.0
C B:GLN226 4.2 31.3 1.0
CA B:GLN226 4.2 30.6 1.0
CG B:GLN226 4.2 32.8 1.0
CD B:ARG177 4.2 25.9 1.0
CB B:ARG177 4.3 26.2 1.0
CB B:THR174 4.4 23.5 1.0
CB B:GLN226 4.8 31.9 1.0
CG B:ARG177 4.9 27.7 1.0

Chlorine binding site 3 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 3 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Cl2642

b:34.7
occ:1.00
 N E:GLN226 3.2 35.1 1.0
NE E:ARG177 3.2 30.6 1.0
CA E:GLY225 3.6 33.1 1.0
NH2 E:ARG177 3.6 33.7 1.0
O E:GLN226 3.6 33.9 1.0
CG2 E:VAL178 3.8 28.9 1.0
CZ E:ARG177 3.9 30.3 1.0
C E:GLY225 3.9 34.2 1.0
CG2 E:THR174 4.0 30.1 1.0
CA E:GLN226 4.2 36.5 1.0
CB E:THR174 4.2 28.5 1.0
CD E:ARG177 4.3 28.4 1.0
C E:GLN226 4.3 36.2 1.0
CB E:ARG177 4.4 30.0 1.0
CB E:GLN226 4.6 38.2 1.0
CG E:GLN226 4.8 42.7 1.0
NH1 E:ARG236 4.8 60.5 1.0
N E:GLY225 4.9 31.7 1.0
CG E:ARG177 5.0 29.4 1.0

Chlorine binding site 4 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 4 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Cl2644

b:28.8
occ:1.00
 N G:GLN226 3.2 27.5 1.0
NE G:ARG177 3.3 25.3 1.0
CA G:GLY225 3.5 27.2 1.0
CG2 G:VAL178 3.6 24.9 1.0
O G:GLN226 3.7 27.4 1.0
NH2 G:ARG177 3.7 28.2 1.0
C G:GLY225 3.9 26.8 1.0
CZ G:ARG177 3.9 24.4 1.0
CG2 G:THR174 4.1 21.7 1.0
CA G:GLN226 4.2 28.7 1.0
CB G:THR174 4.3 23.3 1.0
CD G:ARG177 4.3 21.9 1.0
CB G:GLN226 4.3 30.3 1.0
C G:GLN226 4.4 27.8 1.0
CB G:ARG177 4.4 24.4 1.0
CG G:GLN226 4.5 35.0 1.0
N G:GLY225 4.9 26.4 1.0
O G:TYR224 4.9 24.5 1.0
CG G:ARG177 5.0 24.4 1.0

Chlorine binding site 5 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 5 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl2650

b:32.5
occ:1.00
 N C:GLN226 3.1 30.6 1.0
NE C:ARG177 3.2 34.0 1.0
CA C:GLY225 3.6 29.7 1.0
O C:GLN226 3.6 31.5 1.0
NH2 C:ARG177 3.6 35.2 1.0
CG2 C:VAL178 3.7 25.1 1.0
C C:GLY225 3.8 29.8 1.0
CZ C:ARG177 3.9 35.2 1.0
CA C:GLN226 4.1 32.1 1.0
CG2 C:THR174 4.2 25.2 1.0
CD C:ARG177 4.2 33.1 1.0
CB C:THR174 4.2 29.0 1.0
C C:GLN226 4.3 32.3 1.0
CB C:ARG177 4.3 31.0 1.0
CB C:GLN226 4.5 33.7 1.0
CG C:GLN226 4.8 36.1 1.0
CG C:ARG177 4.9 32.2 1.0
N C:GLY225 5.0 27.6 1.0

Chlorine binding site 6 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 6 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 6 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cl2641

b:37.2
occ:1.00
 NE D:ARG177 2.9 40.3 1.0
NH2 D:ARG177 3.4 42.4 1.0
N D:GLN226 3.5 33.5 1.0
CZ D:ARG177 3.6 38.8 1.0
CG2 D:VAL178 3.6 27.1 1.0
O D:GLN226 3.7 34.3 1.0
CA D:GLY225 3.8 32.4 1.0
CD D:ARG177 4.0 36.5 1.0
CB D:ARG177 4.1 29.8 1.0
C D:GLY225 4.2 32.2 1.0
CG2 D:THR174 4.3 27.1 1.0
CB D:THR174 4.3 29.6 1.0
NH2 D:ARG236 4.4 58.7 1.0
CA D:GLN226 4.4 35.1 1.0
CB D:GLN226 4.4 36.1 1.0
C D:GLN226 4.5 34.9 1.0
CG D:ARG177 4.7 31.9 1.0
NH1 D:ARG177 4.9 39.4 1.0

Chlorine binding site 7 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 7 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 7 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Cl2643

b:35.5
occ:1.00
 NE F:ARG177 3.2 37.0 1.0
N F:GLN226 3.2 37.2 1.0
O F:GLN226 3.5 38.9 1.0
NH2 F:ARG177 3.6 42.9 1.0
CA F:GLY225 3.7 33.2 1.0
CZ F:ARG177 3.8 41.5 1.0
CG2 F:VAL178 3.9 27.0 1.0
C F:GLY225 4.0 34.5 1.0
CG2 F:THR174 4.0 31.8 1.0
CA F:GLN226 4.1 39.2 1.0
CD F:ARG177 4.2 34.1 1.0
C F:GLN226 4.2 39.9 1.0
CB F:THR174 4.2 31.3 1.0
CB F:ARG177 4.4 30.7 1.0
CB F:GLN226 4.4 40.5 1.0
CG F:ARG177 4.9 33.1 1.0

Chlorine binding site 8 out of 8 in 3ezw

Go back to Chlorine Binding Sites List in 3ezw
Chlorine binding site 8 out of 8 in the Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 8 of Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices within 5.0Å range:
probe atom residue distance (Å) B Occ
H:Cl2645

b:39.0
occ:1.00
 NE H:ARG177 3.0 34.2 1.0
NH2 H:ARG177 3.3 38.7 1.0
N H:GLN226 3.3 35.1 1.0
O H:GLN226 3.5 35.6 1.0
CZ H:ARG177 3.6 33.9 1.0
CA H:GLY225 3.8 31.3 1.0
CG2 H:VAL178 3.8 25.8 1.0
CG2 H:THR174 4.0 28.6 1.0
C H:GLY225 4.1 32.1 1.0
CB H:ARG177 4.1 30.8 1.0
CD H:ARG177 4.1 32.5 1.0
CG H:GLN226 4.1 42.4 1.0
CB H:THR174 4.2 29.7 1.0
CA H:GLN226 4.3 37.5 1.0
C H:GLN226 4.3 38.4 1.0
CG H:ARG177 4.7 32.4 1.0
CB H:GLN226 4.8 38.1 1.0
NH1 H:ARG177 4.9 35.2 1.0

Reference:

M.J.Anderson, B.Delabarre, A.Raghunathan, B.O.Palsson, A.T.Brunger, S.R.Quake. Crystal Structure of A Hyperactive Escherichia Coli Glycerol Kinase Mutant GLY230 --> Asp Obtained Using Microfluidic Crystallization Devices. Biochemistry V. 46 5722 2007.
ISSN: ISSN 0006-2960
PubMed: 17441732
DOI: 10.1021/BI700096P
Page generated: Sat Jul 20 19:03:08 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy