Chlorine in PDB 3h84: Crystal Structure of GET3

All present enzymatic activity of Crystal Structure of GET3:
3.6.3.16;

The structure of Crystal Structure of GET3, PDB code: 3h84 was solved by J.Hu, J.Li, X.Qian, B.Sha, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.30
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	219.400, 113.743, 48.370, 90.00, 90.00, 90.00
R / Rfree (%)	22.2 / 24.7

The structure of Crystal Structure of GET3 also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Zinc	(Zn)	1 atom
Sodium	(Na)	3 atoms

The binding sites of Chlorine atom in the Crystal Structure of GET3 (pdb code 3h84). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of GET3, PDB code: 3h84:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of GET3


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of GET3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl356 b:73.8 occ:1.00 O B:ASP166 3.4 47.6 1.0 O B:HOH751 3.6 50.9 1.0 CG2 B:THR56 3.8 44.2 1.0 O B:ILE54 4.0 42.6 1.0 OG1 B:THR56 4.0 44.8 1.0 C B:ASP166 4.0 47.6 1.0 CD1 B:PHE165 4.2 45.0 1.0 CA B:THR167 4.2 49.0 1.0 CB B:PHE165 4.3 45.6 1.0 N B:THR56 4.4 44.0 1.0 N B:THR167 4.4 48.3 1.0 CB B:THR56 4.5 44.4 1.0 N B:ASP166 4.5 46.6 1.0 CA B:PHE165 4.6 45.8 1.0 C B:SER55 4.6 43.6 1.0 C B:ILE54 4.6 42.5 1.0 C B:PHE165 4.6 46.0 1.0 CA B:SER55 4.7 43.4 1.0 CG B:PHE165 4.7 45.4 1.0 CG2 B:ILE54 4.8 42.0 1.0 O B:HOH391 4.9 38.2 1.0 CA B:ASP166 4.9 47.3 1.0 N B:SER55 5.0 43.0 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of GET3


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of GET3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl357 b:68.0 occ:1.00 N B:THR173 3.9 54.2 1.0 O B:HOH416 4.0 56.0 1.0 OE2 B:GLU258 4.0 51.0 1.0 OG1 B:THR173 4.0 54.3 1.0 N B:LEU174 4.1 52.6 1.0 OE1 B:GLU258 4.2 50.8 1.0 CD2 B:LEU255 4.3 48.0 1.0 CB B:LEU174 4.3 52.0 1.0 C B:HIS172 4.4 54.4 1.0 CE1 B:PHE238 4.4 42.4 1.0 CA B:HIS172 4.4 54.7 1.0 CD B:GLU258 4.4 50.3 1.0 O B:HOH752 4.4 47.7 1.0 CD1 B:LEU174 4.5 52.1 1.0 CG B:LEU174 4.6 51.7 1.0 CZ B:PHE238 4.6 42.2 1.0 CD2 B:LEU259 4.7 47.8 1.0 CA B:THR173 4.7 53.9 1.0 CA B:LEU174 4.8 52.1 1.0 C B:THR173 4.9 53.3 1.0 O B:GLY171 4.9 55.0 1.0

J.Hu, J.Li, X.Qian, V.Denic, B.Sha. The Crystal Structures of Yeast GET3 Suggest A Mechanism For Tail-Anchored Protein Membrane Insertion. Plos One V. 4 E8061 2009.
ISSN: ESSN 1932-6203
PubMed: 19956640
DOI: 10.1371/JOURNAL.PONE.0008061