Chlorine in PDB 3hnb: Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor

The structure of Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor, PDB code: 3hnb was solved by Z.Liu, C.Yuan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.80 / 1.15
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	42.054, 55.775, 67.971, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 22

The binding sites of Chlorine atom in the Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor (pdb code 3hnb). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor, PDB code: 3hnb:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor within 5.0Å range: probe atom residue distance (Å) B Occ M:Cl1 b:31.0 occ:1.00 CL1 M:7681 0.0 31.0 1.0 C21 M:7681 1.5 30.7 1.0 C22 M:7681 2.5 30.7 1.0 C20 M:7681 2.5 30.6 1.0 NE2 M:HIS2315 3.2 7.1 0.2 CD2 M:HIS2315 3.5 7.2 0.2 C23 M:7681 3.7 30.3 1.0 C19 M:7681 3.8 30.6 1.0 C18 M:7681 4.3 30.7 1.0 CE1 M:HIS2315 4.4 6.8 0.2 NE2 M:HIS2315 4.6 13.2 0.3 CD2 M:HIS2315 4.7 12.4 0.3 CG M:HIS2315 4.8 8.0 0.2 CL2 M:7681 4.9 30.2 1.0

Chlorine binding site 2 out of 2 in the Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Factor VIII TRP2313-HIS2315 Segment Is Involved in Membrane Binding As Shown By Crystal Structure of Complex Between Factor VIII C2 Domain and An Inhibitor within 5.0Å range: probe atom residue distance (Å) B Occ M:Cl1 b:30.2 occ:1.00 CL2 M:7681 0.0 30.2 1.0 C23 M:7681 1.5 30.3 1.0 C22 M:7681 2.4 30.7 1.0 C18 M:7681 2.6 30.7 1.0 C12 M:7681 2.9 28.7 1.0 O2 M:7681 3.0 31.1 1.0 N M:HIS2315 3.2 11.1 0.5 N M:HIS2315 3.2 10.2 0.2 N M:HIS2315 3.3 10.9 0.3 C11 M:7681 3.6 28.8 1.0 C21 M:7681 3.7 30.7 1.0 O M:TRP2313 3.8 10.7 1.0 CA M:HIS2315 3.8 9.7 0.2 CA M:HIS2315 3.8 11.0 0.5 C19 M:7681 3.9 30.6 1.0 C17 M:7681 3.9 30.7 1.0 C15 M:7681 4.0 29.4 1.0 C13 M:7681 4.0 28.6 1.0 CA M:HIS2315 4.0 10.9 0.3 C16 M:7681 4.1 30.5 1.0 C M:VAL2314 4.2 10.5 1.0 CA M:VAL2314 4.2 10.8 1.0 C20 M:7681 4.3 30.6 1.0 N3 M:7681 4.4 28.7 1.0 O M:HIS2315 4.5 10.9 0.3 C M:HIS2315 4.6 9.8 0.2 O M:HIS2315 4.6 10.0 0.2 CD2 M:HIS2315 4.6 7.2 0.2 C M:HIS2315 4.6 10.6 0.3 C M:TRP2313 4.7 10.1 1.0 C M:HIS2315 4.8 10.7 0.5 CL1 M:7681 4.9 31.0 1.0 CB M:HIS2315 4.9 11.3 0.5 CD1 M:TRP2313 5.0 11.7 1.0 N M:VAL2314 5.0 10.1 1.0 C10 M:7681 5.0 28.8 1.0

Z.Liu, L.Lin, C.Yuan, G.A.Nicolaes, L.Chen, E.J.Meehan, B.Furie, B.Furie, M.Huang. TRP2313-HIS2315 of Factor VIII C2 Domain Is Involved in Membrane Binding: Structure of A Complex Between the C2 Domain and An Inhibitor of Membrane Binding. J.Biol.Chem. V. 285 8824 2010.
ISSN: ISSN 0021-9258
PubMed: 20089867
DOI: 10.1074/JBC.M109.080168