Chlorine in PDB 3lda: Yeast RAD51 H352Y Filament Interface Mutant

The structure of Yeast RAD51 H352Y Filament Interface Mutant, PDB code: 3lda was solved by N.L.Villanueva, J.Chen, S.W.Morrical, M.A.Rould, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.50
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	78.905, 78.905, 130.333, 90.00, 90.00, 120.00
R / Rfree (%)	20 / 24.6

The binding sites of Chlorine atom in the Yeast RAD51 H352Y Filament Interface Mutant (pdb code 3lda). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Yeast RAD51 H352Y Filament Interface Mutant, PDB code: 3lda:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Yeast RAD51 H352Y Filament Interface Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Yeast RAD51 H352Y Filament Interface Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1080 b:42.8 occ:1.00 NE A:ARG154 3.3 31.4 1.0 CD A:ARG154 3.7 32.3 1.0 CD2 A:HIS151 3.8 48.1 1.0 NE2 A:HIS151 4.4 50.9 1.0 CZ A:ARG154 4.4 31.5 1.0 CG A:HIS151 4.4 45.1 1.0 CB A:ARG154 4.6 34.7 1.0 CA A:HIS151 4.6 35.8 1.0 NH1 A:ARG154 4.7 30.3 1.0 CG A:ARG154 4.8 32.9 1.0 CB A:HIS151 4.9 39.4 1.0

Chlorine binding site 2 out of 3 in the Yeast RAD51 H352Y Filament Interface Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Yeast RAD51 H352Y Filament Interface Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1081 b:64.2 occ:1.00 N A:LYS191 3.3 34.9 1.0 CB A:LYS191 3.3 32.9 1.0 N A:ARG188 3.4 45.8 1.0 NZ A:LYS191 3.4 41.3 1.0 N A:THR189 3.4 41.1 1.0 N A:SER192 3.5 36.2 1.0 CE A:LYS191 3.7 35.9 1.0 CA A:LYS191 3.7 35.1 1.0 N A:GLY190 3.8 37.6 1.0 CG A:LYS191 3.9 32.8 1.0 OG1 A:THR189 3.9 40.1 1.0 CA A:ARG188 3.9 45.2 1.0 O A:GLU186 4.0 43.3 1.0 C A:ARG188 4.1 43.5 1.0 C A:LYS191 4.2 35.9 1.0 C A:GLY190 4.2 36.3 1.0 C A:PHE187 4.2 46.0 1.0 CA A:PHE187 4.3 46.8 1.0 CA A:THR189 4.3 39.6 1.0 C A:THR189 4.4 38.0 1.0 CB A:SER192 4.4 39.6 1.0 CD A:LYS191 4.4 34.7 1.0 CA A:GLY190 4.5 36.7 1.0 CA A:SER192 4.6 36.9 1.0 CB A:THR189 4.8 39.7 1.0 OE1 A:GLN326 4.8 62.6 1.0 C A:GLU186 5.0 43.0 1.0

Chlorine binding site 3 out of 3 in the Yeast RAD51 H352Y Filament Interface Mutant


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Yeast RAD51 H352Y Filament Interface Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1082 b:62.9 occ:1.00 O A:ARG188 3.1 44.8 1.0 N A:GLY190 3.2 37.6 1.0 C A:ARG188 3.2 43.5 1.0 NH1 A:ARG368 3.5 39.9 1.0 NE A:ARG368 3.5 43.1 1.0 CA A:ARG188 3.5 45.2 1.0 CA A:GLY190 3.7 36.7 1.0 CG A:ARG188 3.7 47.1 0.5 N A:THR189 3.8 41.1 1.0 CZ A:ARG368 4.0 42.1 1.0 C A:THR189 4.1 38.0 1.0 CB A:ARG188 4.2 46.6 1.0 CA A:THR189 4.4 39.6 1.0 CG A:GLN193 4.4 37.6 1.0 CD A:ARG368 4.7 40.6 1.0 NH2 A:ARG188 4.7 49.8 0.5 N A:ARG188 4.7 45.8 1.0 CG1 A:ILE387 4.7 43.0 1.0 C A:GLY190 4.8 36.3 1.0

J.Chen, N.Villanueva, M.A.Rould, S.W.Morrical. Insights Into the Mechanism of RAD51 Recombinase From the Structure and Properties of A Filament Interface Mutant. Nucleic Acids Res. V. 38 4889 2010.
ISSN: ISSN 0305-1048
PubMed: 20371520
DOI: 10.1093/NAR/GKQ209