Chlorine in PDB 3m12: Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase

All present enzymatic activity of Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase:
1.5.3.1;

The structure of Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase, PDB code: 3m12 was solved by F.S.Mathews, Z.-W.Chen, M.S.Jorns, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.89 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.323, 69.251, 73.232, 90.00, 93.68, 90.00
R / Rfree (%)	17.9 / 21.5

The binding sites of Chlorine atom in the Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase (pdb code 3m12). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase, PDB code: 3m12:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl405 b:15.2 occ:1.00 O3' A:FAD400 3.0 12.5 1.0 O A:HOH432 3.0 12.4 1.0 N A:GLY344 3.2 13.8 1.0 N A:THR318 3.3 14.7 1.0 N A:SER343 3.5 14.0 1.0 C3' A:FAD400 3.6 12.8 1.0 CA A:TYR317 3.6 14.7 1.0 C A:PHE342 3.7 13.6 1.0 CA A:GLY344 3.8 13.6 1.0 C A:TYR317 3.9 15.0 1.0 CB A:PHE342 4.0 13.2 1.0 O A:HOH406 4.1 12.7 1.0 CA A:PHE342 4.1 13.4 1.0 CB A:TYR317 4.2 14.5 1.0 C A:SER343 4.2 14.0 1.0 C5' A:FAD400 4.2 13.5 1.0 O A:THR318 4.2 15.1 1.0 O A:PHE342 4.2 13.8 1.0 CA A:SER343 4.2 14.2 1.0 O A:MET316 4.3 14.7 1.0 N A:PHE342 4.3 13.6 1.0 CA A:THR318 4.4 15.2 1.0 C1' A:FAD400 4.4 12.8 1.0 C4' A:FAD400 4.5 13.0 1.0 CB A:THR318 4.6 15.3 1.0 C A:THR318 4.7 15.4 1.0 CD1 A:TYR317 4.7 13.4 1.0 C2' A:FAD400 4.7 11.9 1.0 OG A:SER43 4.7 13.5 1.0 OG1 A:THR318 4.7 15.8 1.0 CG A:TYR317 4.8 14.3 1.0 C A:GLY344 4.8 13.4 1.0 N A:TYR317 4.8 14.7 1.0 N A:HIS345 4.9 13.1 1.0 CB A:SER43 4.9 13.3 1.0 C A:MET316 4.9 14.9 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the LYS265ARG Phosphate-Crytsallized Mutant of Monomeric Sarcosine Oxidase within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl406 b:13.6 occ:1.00 O B:HOH412 3.1 12.4 1.0 O3' B:FAD400 3.1 11.5 1.0 N B:GLY344 3.2 12.6 1.0 N B:THR318 3.3 13.3 1.0 N B:SER343 3.5 12.5 1.0 CA B:TYR317 3.6 13.2 1.0 C3' B:FAD400 3.6 11.5 1.0 C B:PHE342 3.7 12.2 1.0 CA B:GLY344 3.9 12.4 1.0 C B:TYR317 3.9 13.4 1.0 CB B:PHE342 4.0 11.8 1.0 O B:HOH409 4.0 12.0 1.0 CA B:PHE342 4.1 12.0 1.0 O B:MET316 4.2 13.3 1.0 C B:SER343 4.2 13.0 1.0 O B:PHE342 4.2 12.4 1.0 CB B:TYR317 4.2 13.5 1.0 CA B:SER343 4.2 13.0 1.0 C5' B:FAD400 4.3 12.1 1.0 O B:THR318 4.3 12.7 1.0 N B:PHE342 4.3 12.2 1.0 CA B:THR318 4.4 13.4 1.0 C1' B:FAD400 4.4 13.1 1.0 CB B:THR318 4.6 13.5 1.0 C4' B:FAD400 4.6 12.3 1.0 CD1 B:TYR317 4.6 12.9 1.0 OG B:SER43 4.7 12.6 1.0 C B:THR318 4.7 13.4 1.0 C2' B:FAD400 4.7 12.5 1.0 N B:TYR317 4.7 13.2 1.0 CG B:TYR317 4.7 13.3 1.0 OG1 B:THR318 4.8 14.8 1.0 C B:GLY344 4.8 12.8 1.0 C B:MET316 4.8 13.5 1.0 CB B:SER43 4.9 12.3 1.0 N B:HIS345 4.9 12.3 1.0

M.S.Jorns, Z.W.Chen, F.S.Mathews. Structural Characterization of Mutations at the Oxygen Activation Site in Monomeric Sarcosine Oxidase. Biochemistry V. 49 3631 2010.
ISSN: ISSN 0006-2960
PubMed: 20353187
DOI: 10.1021/BI100160J