Chlorine in PDB 3m24: Crystal Structure of Tagbfp Fluorescent Protein

The structure of Crystal Structure of Tagbfp Fluorescent Protein, PDB code: 3m24 was solved by V.N.Malashkevich, O.M.Subach, S.C.Almo, V.V.Verkhusha, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.94 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	82.953, 105.943, 137.155, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 23.8

The binding sites of Chlorine atom in the Crystal Structure of Tagbfp Fluorescent Protein (pdb code 3m24). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Tagbfp Fluorescent Protein, PDB code: 3m24:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Tagbfp Fluorescent Protein


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Tagbfp Fluorescent Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl508 b:11.6 occ:1.00 O B:HOH421 3.1 22.9 1.0 O A:HOH427 3.3 31.7 1.0 N B:ASP159 3.3 9.8 1.0 N A:ASP159 3.3 7.3 1.0 CA A:ASN158 3.6 6.1 1.0 CA B:ASN158 3.6 9.0 1.0 CB A:ASN158 3.9 6.5 1.0 O B:HOH396 3.9 11.0 1.0 CB B:ASN158 4.0 10.1 1.0 C A:ASN158 4.0 6.6 1.0 C B:ASN158 4.0 9.4 1.0 O A:HOH390 4.1 13.4 1.0 OD2 B:ASP159 4.3 12.2 1.0 CB A:ASP159 4.4 9.9 1.0 CB B:ASP159 4.4 11.2 1.0 CA B:ASP159 4.4 10.3 1.0 O A:THR144 4.4 7.0 1.0 CA A:ASP159 4.4 8.6 1.0 O B:ASP159 4.4 9.8 1.0 O B:THR144 4.4 6.1 1.0 O A:ASP159 4.4 10.1 1.0 OD2 A:ASP159 4.5 11.8 1.0 CG B:ASP159 4.8 16.1 1.0 N A:ASN158 4.8 5.1 1.0 CG A:ASP159 4.8 18.6 1.0 N B:ASN158 4.8 7.6 1.0 C B:ASP159 4.9 9.6 1.0 C A:ASP159 4.9 9.1 1.0 O A:ARG157 5.0 5.2 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Tagbfp Fluorescent Protein


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Tagbfp Fluorescent Protein within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl509 b:14.6 occ:1.00 O C:HOH388 3.1 19.1 1.0 N C:ASP159 3.3 11.2 1.0 N D:ASP159 3.4 14.4 1.0 O C:HOH357 3.4 5.0 1.0 CA D:ASN158 3.7 13.5 1.0 CA C:ASN158 3.7 10.2 1.0 O D:HOH354 3.8 18.4 1.0 CB C:ASN158 3.9 10.7 1.0 C C:ASN158 4.0 10.7 1.0 CB D:ASN158 4.0 13.6 1.0 C D:ASN158 4.1 13.8 1.0 O C:THR144 4.2 8.4 1.0 CB C:ASP159 4.2 14.2 1.0 CA C:ASP159 4.3 12.3 1.0 CB D:ASP159 4.3 16.3 1.0 O C:ASP159 4.4 11.5 1.0 O D:THR144 4.4 12.3 1.0 CA D:ASP159 4.4 15.6 1.0 OD2 D:ASP159 4.4 17.4 1.0 OD2 C:ASP159 4.6 15.9 1.0 O D:ASP159 4.6 16.9 1.0 CG D:ASP159 4.8 26.1 1.0 C C:ASP159 4.8 11.6 1.0 N D:ASN158 4.8 13.4 1.0 CG C:ASP159 4.9 26.9 1.0 N C:ASN158 4.9 9.1 1.0 C D:ASP159 5.0 16.0 1.0

O.M.Subach, V.N.Malashkevich, W.D.Zencheck, K.S.Morozova, K.D.Piatkevich, S.C.Almo, V.V.Verkhusha. Structural Characterization of Acylimine-Containing Blue and Red Chromophores in Mtagbfp and Tagrfp Fluorescent Proteins. Chem.Biol. V. 17 333 2010.
ISSN: ISSN 1074-5521
PubMed: 20416505
DOI: 10.1016/J.CHEMBIOL.2010.03.005