Chlorine in PDB 3mzr: Rnase Crystals Grown in Loops/Micromounts

All present enzymatic activity of Rnase Crystals Grown in Loops/Micromounts:
3.1.27.5;

The structure of Rnase Crystals Grown in Loops/Micromounts, PDB code: 3mzr was solved by I.I.Mathews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.50
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	64.190, 64.190, 63.800, 90.00, 90.00, 120.00
R / Rfree (%)	17.6 / 19.3

The binding sites of Chlorine atom in the Rnase Crystals Grown in Loops/Micromounts (pdb code 3mzr). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Rnase Crystals Grown in Loops/Micromounts, PDB code: 3mzr:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Rnase Crystals Grown in Loops/Micromounts


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Rnase Crystals Grown in Loops/Micromounts within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl125 b:14.3 occ:1.00 OG1 A:THR3 3.1 10.4 1.0 O A:HOH180 3.1 13.5 1.0 CB A:THR3 4.1 9.7 1.0 CG2 A:THR3 4.1 10.8 1.0 CB A:ALA5 4.2 10.1 1.0 N A:ALA6 4.2 8.0 1.0 CB A:ALA6 4.2 7.6 1.0 O A:HOH187 4.3 12.7 1.0 CA A:ALA6 4.7 7.9 1.0 O A:HOH166 4.8 11.7 1.0 C A:ALA5 4.9 8.9 1.0

Chlorine binding site 2 out of 4 in the Rnase Crystals Grown in Loops/Micromounts


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Rnase Crystals Grown in Loops/Micromounts within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl126 b:21.6 occ:1.00 O A:HOH162 2.9 10.8 1.0 CB A:SER15 3.8 14.2 1.0 CB A:SER50 3.8 9.4 1.0 CA A:SER50 3.9 8.0 1.0 O A:SER15 4.3 20.9 1.0 OG A:SER15 4.3 12.3 1.0 N A:SER50 4.7 8.8 1.0 O A:HOH147 4.8 10.6 1.0 O A:HOH218 4.8 29.5 1.0 CA A:SER15 4.9 13.8 1.0 C A:SER15 4.9 16.5 1.0

Chlorine binding site 3 out of 4 in the Rnase Crystals Grown in Loops/Micromounts


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Rnase Crystals Grown in Loops/Micromounts within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl127 b:35.3 occ:1.00 O A:HOH198 3.2 13.7 1.0 CG A:GLU2 3.8 13.6 1.0 CB A:ALA6 3.9 7.6 1.0 OE2 A:GLU2 4.2 13.2 1.0 CD A:GLU2 4.5 13.6 1.0 CB A:GLU2 4.5 14.2 1.0 CA A:GLU2 4.5 13.7 1.0 O A:HOH171 4.8 13.3 1.0

Chlorine binding site 4 out of 4 in the Rnase Crystals Grown in Loops/Micromounts


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Rnase Crystals Grown in Loops/Micromounts within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl128 b:15.4 occ:1.00 O A:HOH131 3.1 28.8 1.0 N A:THR45 3.3 8.7 1.0 CE1 A:HIS12 3.5 10.8 1.0 CA A:ASN44 3.7 9.0 1.0 O A:HOH264 3.7 26.8 1.0 OG1 A:THR45 3.7 13.0 1.0 O A:HOH259 3.8 29.2 1.0 C A:ASN44 3.9 9.1 1.0 CD1 A:PHE120 3.9 13.7 1.0 CB A:THR45 4.0 8.9 1.0 ND1 A:HIS12 4.2 9.4 1.0 O A:VAL43 4.2 12.5 1.0 CA A:THR45 4.2 8.9 1.0 N A:ASN44 4.3 9.9 1.0 NZ A:LYS41 4.3 22.5 1.0 CB A:PHE120 4.4 12.2 1.0 OD1 A:ASN44 4.4 12.8 1.0 CG A:PHE120 4.4 11.0 1.0 NE2 A:HIS12 4.5 10.8 1.0 C A:VAL43 4.6 11.7 1.0 O A:PHE120 4.6 14.3 1.0 CG1 A:VAL43 4.7 12.7 1.0 CE1 A:PHE120 4.7 14.0 1.0 CB A:ASN44 4.8 10.2 1.0 CG A:ASN44 4.8 12.6 1.0 O A:THR45 4.9 8.5 1.0

M.A.Berger, J.H.Decker, I.I.Mathews. Diffraction Study of Protein Crystals Grown in Cryoloops and Micromounts. J.Appl.Crystallogr. V. 43 1513 2010.
ISSN: ISSN 0021-8898
PubMed: 22477781
DOI: 10.1107/S0021889810040409