Chlorine in PDB 3pku: Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide

All present enzymatic activity of Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide:
1.7.3.3;

The structure of Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide, PDB code: 3pku was solved by G.Marassio, N.Colloc'h, T.Prange, J.H.Abraini, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.97 / 1.75
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	80.120, 96.211, 105.456, 90.00, 90.00, 90.00
R / Rfree (%)	16.3 / 19.6

The structure of Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Chlorine atom in the Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide (pdb code 3pku). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide, PDB code: 3pku:

Chlorine binding site 1 out of 1 in the Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Urate Oxidase Under 1 Mpa / 10 Bars Pressure of Nitrous Oxide within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl402 b:23.6 occ:1.00 ND2 A:ASN254 3.0 21.0 1.0 CA A:GLY286 3.4 11.1 1.0 CB A:ASN254 3.5 14.9 1.0 C4 A:AZA302 3.5 13.9 1.0 C5 A:AZA302 3.5 14.2 1.0 CE1 A:HIS256 3.6 18.3 1.0 CG A:ASN254 3.7 19.0 1.0 N9 A:AZA302 3.7 14.2 1.0 N7 A:AZA302 3.8 13.7 1.0 N8 A:AZA302 3.9 14.3 1.0 N3 A:AZA302 3.9 13.6 1.0 N A:GLY286 4.0 12.0 1.0 C6 A:AZA302 4.0 14.3 1.0 NE2 A:HIS256 4.1 18.3 1.0 C2 A:AZA302 4.3 12.9 1.0 N1 A:AZA302 4.4 13.5 1.0 CD1 A:ILE288 4.4 20.6 1.0 C A:GLY286 4.4 10.3 1.0 O6 A:AZA302 4.7 16.1 1.0 ND1 A:HIS256 4.8 18.1 1.0 CA A:ASN254 4.9 14.2 1.0 OD1 A:ASN254 4.9 21.4 1.0 N A:LEU287 4.9 10.8 1.0 O A:HOH409 4.9 16.8 1.0

G.Marassio, T.Prange, H.N.David, J.Sopkova-De Oliveira Santos, L.Gabison, N.Delcroix, J.H.Abraini, N.Colloc'h. Pressure-Response Analysis of Anesthetic Gases Xenon and Nitrous Oxide on Urate Oxidase: A Crystallographic Study. Faseb J. V. 25 2266 2011.
ISSN: ISSN 0892-6638
PubMed: 21421845
DOI: 10.1096/FJ.11-183046