Chlorine in PDB 3sg8: Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex

The structure of Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex, PDB code: 3sg8 was solved by K.Shi, D.R.Houston, A.M.Berghuis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.99 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	43.334, 101.479, 73.455, 90.00, 100.78, 90.00
R / Rfree (%)	18.5 / 24.2

The binding sites of Chlorine atom in the Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex (pdb code 3sg8). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex, PDB code: 3sg8:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl306 b:30.7 occ:1.00 NZ A:LYS121 3.1 40.0 1.0 CE1 A:HIS251 3.5 29.3 1.0 CE A:LYS121 3.7 35.8 1.0 ND1 A:HIS251 3.9 26.5 1.0 CD A:LYS121 4.0 28.8 1.0 NE2 A:HIS251 4.7 28.1 1.0 O A:HOH471 4.9 20.5 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type Iva Tobramycin Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl306 b:34.1 occ:1.00 O B:HOH311 3.0 12.9 1.0 NZ B:LYS70 3.0 50.3 1.0 N B:ASN134 3.2 30.0 1.0 CG2 B:ILE133 3.8 24.8 1.0 CB B:ASN134 3.9 35.9 1.0 CE B:LYS70 4.0 47.1 1.0 CA B:ILE133 4.0 24.2 1.0 C B:ILE133 4.1 26.4 1.0 CA B:ASN134 4.1 33.8 1.0 CD B:LYS70 4.1 39.1 1.0 CB B:ILE133 4.4 23.8 1.0 CG1 B:ILE133 4.6 24.3 1.0 O B:ASN134 4.8 33.8 1.0 C B:ASN134 5.0 34.0 1.0

K.Shi, D.R.Houston, A.M.Berghuis. Crystal Structures of Antibiotic-Bound Complexes of Aminoglycoside 2''-Phosphotransferase Iva Highlight the Diversity in Substrate Binding Modes Among Aminoglycoside Kinases. Biochemistry V. 50 6237 2011.
ISSN: ISSN 0006-2960
PubMed: 21678960
DOI: 10.1021/BI200747F