Chlorine in PDB 3t0w: Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red

The structure of Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red, PDB code: 3t0w was solved by R.Stanfield, N.Senutovitch, S.Bhattacharyya, G.Rule, I.A.Wilson, B.Armitage, A.S.Waggoner, P.Berget, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.77 / 1.50
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	73.899, 83.533, 35.142, 90.00, 90.00, 90.00
R / Rfree (%)	14.8 / 18.3

The binding sites of Chlorine atom in the Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red (pdb code 3t0w). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red, PDB code: 3t0w:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl201 b:17.7 occ:1.00 O A:HOH341 3.1 14.7 1.0 N A:PHE22 3.1 5.9 1.0 CA A:ILE21 3.5 7.8 1.0 O A:HOH381 3.5 17.3 1.0 CB A:ILE21 3.8 10.2 1.0 C A:ILE21 3.8 6.6 1.0 CG2 A:VAL11 4.0 15.4 1.0 OG A:SER7 4.1 12.6 0.6 CB A:SER7 4.1 12.9 0.4 CD2 A:PHE22 4.1 6.9 1.0 CA A:PHE22 4.1 5.8 1.0 CG2 A:ILE21 4.1 13.3 1.0 CB A:PHE22 4.1 5.9 1.0 CB A:SER7 4.1 11.5 0.6 O A:PHE22 4.3 8.5 1.0 O A:THR20 4.4 8.5 1.0 O A:HOH398 4.4 12.6 1.0 CG A:PHE22 4.6 6.2 1.0 C A:PHE22 4.7 7.4 1.0 N A:ILE21 4.8 6.9 1.0 N A:SER7 4.8 9.2 1.0 OG1 A:THR102 4.8 8.4 1.0 OG A:SER7 4.9 15.0 0.4

Chlorine binding site 2 out of 2 in the Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Fluorogen Activating Protein M8VL in Complex with Dimethylindole Red within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:23.1 occ:1.00 CE2 A:PHE22 4.0 8.6 1.0 CZ A:PHE22 4.4 9.4 1.0 CD2 A:PHE22 4.9 6.9 1.0 O A:HOH381 4.9 17.3 1.0

N.Senutovitch, R.L.Stanfield, S.Bhattacharyya, G.S.Rule, I.A.Wilson, B.A.Armitage, A.S.Waggoner, P.B.Berget. A Variable Light Domain Fluorogen Activating Protein Homodimerizes to Activate Dimethylindole Red. Biochemistry V. 51 2471 2012.
ISSN: ISSN 0006-2960
PubMed: 22390683
DOI: 10.1021/BI201422G