Chlorine in PDB 3tlu: The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype)

The structure of The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype), PDB code: 3tlu was solved by L.Sauguet, H.Nury, P.J.Corringer, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.14 / 2.85
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	177.330, 127.810, 159.090, 90.00, 100.77, 90.00
R / Rfree (%)	20.3 / 22.3

The binding sites of Chlorine atom in the The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype) (pdb code 3tlu). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype), PDB code: 3tlu:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl318 b:56.3 occ:1.00 O A:HOH325 3.0 66.8 1.0 NH1 A:ARG85 3.3 57.9 1.0 N A:ARG85 3.4 73.1 1.0 N A:PHE78 3.5 66.0 1.0 CG A:ARG85 3.6 76.6 1.0 CD2 A:PHE78 3.7 76.9 1.0 CB A:ARG85 3.9 69.0 1.0 CA A:ALA84 3.9 76.4 1.0 CA A:ARG77 4.0 62.6 1.0 CD A:ARG85 4.0 75.9 1.0 CB A:PHE78 4.1 68.0 1.0 CG2 A:VAL81 4.1 64.5 1.0 C A:ALA84 4.2 79.0 1.0 CG A:PHE78 4.2 71.3 1.0 C A:ARG77 4.2 67.4 1.0 CA A:ARG85 4.3 72.0 1.0 CB A:ALA84 4.3 77.8 1.0 CZ A:ARG85 4.4 74.3 1.0 CA A:PHE78 4.4 65.7 1.0 CB A:ARG77 4.4 58.5 1.0 CE2 A:PHE78 4.5 80.7 1.0 O A:PHE78 4.6 68.8 1.0 NE A:ARG85 4.6 71.0 1.0

Chlorine binding site 2 out of 5 in the The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl318 b:79.5 occ:1.00 N B:PHE78 3.4 62.6 1.0 NH1 B:ARG85 3.5 56.6 1.0 N B:ARG85 3.5 66.2 1.0 CA B:ALA84 3.8 70.2 1.0 CD2 B:PHE78 3.8 74.5 1.0 CA B:ARG77 3.9 58.8 1.0 CG B:ARG85 4.0 61.8 1.0 CG2 B:VAL81 4.0 62.4 1.0 CB B:ALA84 4.1 71.5 1.0 CB B:PHE78 4.1 65.9 1.0 C B:ARG77 4.1 62.9 1.0 C B:ALA84 4.2 73.4 1.0 CB B:ARG85 4.2 59.7 1.0 CB B:ARG77 4.3 55.4 1.0 CG B:PHE78 4.3 69.6 1.0 CA B:PHE78 4.3 63.2 1.0 CD B:ARG85 4.3 65.1 1.0 O B:PHE78 4.4 61.2 1.0 O B:HOH347 4.5 67.5 1.0 CA B:ARG85 4.5 64.0 1.0 CZ B:ARG85 4.6 69.8 1.0 CE2 B:PHE78 4.7 77.4 1.0 CG B:ARG77 4.9 63.6 1.0 NE B:ARG85 4.9 56.7 1.0 C B:PHE78 4.9 62.9 1.0 N B:ALA84 4.9 71.8 1.0 O B:ASN83 5.0 76.4 1.0

Chlorine binding site 3 out of 5 in the The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype) within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl319 b:50.5 occ:1.00 O C:HOH342 3.0 36.3 1.0 NH1 C:ARG85 3.3 48.4 1.0 N C:ARG85 3.3 66.4 1.0 N C:PHE78 3.5 59.5 1.0 CD2 C:PHE78 3.5 72.4 1.0 CG C:ARG85 3.6 49.3 1.0 CB C:ARG85 3.9 55.9 1.0 CA C:ALA84 3.9 70.8 1.0 CD C:ARG85 4.0 57.3 1.0 CB C:PHE78 4.0 61.4 1.0 CA C:ARG77 4.1 58.3 1.0 CG C:PHE78 4.1 64.9 1.0 CG2 C:VAL81 4.1 65.9 1.0 C C:ALA84 4.2 73.5 1.0 C C:ARG77 4.2 60.4 1.0 CA C:ARG85 4.2 63.8 1.0 CZ C:ARG85 4.3 67.5 1.0 CA C:PHE78 4.3 59.5 1.0 CE2 C:PHE78 4.4 76.0 1.0 CB C:ALA84 4.4 72.0 1.0 CB C:ARG77 4.5 59.3 1.0 O C:PHE78 4.6 66.7 1.0 NE C:ARG85 4.6 60.2 1.0 C C:PHE78 5.0 64.6 1.0

Chlorine binding site 4 out of 5 in the The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype) within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl318 b:62.1 occ:1.00 N D:ARG85 3.4 66.6 1.0 O C:HOH324 3.5 70.2 1.0 N D:PHE78 3.5 63.9 1.0 NH1 D:ARG85 3.5 50.8 1.0 CA D:ALA84 3.8 67.9 1.0 CD2 D:PHE78 3.8 70.4 1.0 CG D:ARG85 3.8 58.0 1.0 CA D:ARG77 4.0 62.7 1.0 CG2 D:VAL81 4.1 67.4 1.0 CB D:ARG85 4.1 62.8 1.0 CB D:ALA84 4.1 69.1 1.0 CB D:PHE78 4.1 65.1 1.0 C D:ALA84 4.1 71.8 1.0 C D:ARG77 4.2 67.7 1.0 CD D:ARG85 4.3 63.9 1.0 CG D:PHE78 4.3 67.6 1.0 CB D:ARG77 4.3 58.8 1.0 CA D:PHE78 4.4 63.5 1.0 CA D:ARG85 4.4 65.6 1.0 O D:PHE78 4.5 65.6 1.0 CZ D:ARG85 4.6 63.3 1.0 CE2 D:PHE78 4.7 73.6 1.0 NE D:ARG85 4.9 57.2 1.0 CG D:ARG77 4.9 63.2 1.0 C D:PHE78 4.9 66.5 1.0 N D:ALA84 4.9 69.0 1.0

Chlorine binding site 5 out of 5 in the The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype)


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of The Glic Pentameric Ligand-Gated Ion Channel LOOP2-24' Oxidized Mutant in A Locally-Closed Conformation (LC1 Subtype) within 5.0Å range: probe atom residue distance (Å) B Occ E:Cl318 b:71.5 occ:1.00 NH1 E:ARG85 3.3 65.8 1.0 N E:ARG85 3.4 70.4 1.0 N E:PHE78 3.5 57.8 1.0 CD2 E:PHE78 3.6 67.8 1.0 CG E:ARG85 3.6 64.8 1.0 CA E:ALA84 3.9 72.1 1.0 CB E:ARG85 3.9 65.6 1.0 CD E:ARG85 4.0 68.7 1.0 CA E:ARG77 4.0 58.9 1.0 CB E:PHE78 4.1 59.1 1.0 CG2 E:VAL81 4.1 68.7 1.0 CG E:PHE78 4.1 62.2 1.0 C E:ALA84 4.2 76.7 1.0 C E:ARG77 4.2 61.7 1.0 CB E:ALA84 4.3 73.3 1.0 CA E:ARG85 4.3 68.5 1.0 CZ E:ARG85 4.4 73.4 1.0 CA E:PHE78 4.4 56.8 1.0 CE2 E:PHE78 4.4 71.4 1.0 CB E:ARG77 4.5 56.8 1.0 O E:PHE78 4.6 61.6 1.0 NE E:ARG85 4.6 56.2 1.0 C E:PHE78 5.0 60.5 1.0

M.S.Prevost, L.Sauguet, H.Nury, C.Van Renterghem, C.Huon, F.Poitevin, M.Baaden, M.Delarue, P.J.Corringer. A Locally Closed Conformation of A Bacterial Pentameric Proton-Gated Ion Channel. Nat.Struct.Mol.Biol. V. 19 642 2012.
ISSN: ISSN 1545-9993
PubMed: 22580559
DOI: 10.1038/NSMB.2307