Chlorine in PDB 3u93: Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form

The structure of Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form, PDB code: 3u93 was solved by J.Kumar, M.L.Mayer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.05 / 1.88
Space group	P 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	130.401, 55.457, 88.861, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 24.8

The structure of Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form also contains other interesting chemical elements:

Zinc

(Zn)

5 atoms

The binding sites of Chlorine atom in the Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form (pdb code 3u93). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form, PDB code: 3u93:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl263 b:44.5 occ:1.00 H B:LEU55 2.4 37.0 1.0 HH22 B:ARG31 2.6 43.3 1.0 HH12 B:ARG31 2.7 47.2 1.0 HA B:ARG54 2.9 37.5 1.0 N B:LEU55 3.3 30.8 1.0 NH2 B:ARG31 3.4 36.1 1.0 HG B:LEU55 3.4 50.3 1.0 HB2 B:LEU55 3.5 44.7 1.0 NH1 B:ARG31 3.5 39.3 1.0 HD12 B:LEU55 3.7 41.5 1.0 CA B:ARG54 3.8 31.3 1.0 HZ B:PHE32 3.9 38.7 1.0 CZ B:ARG31 3.9 33.3 1.0 C B:ARG54 4.0 37.2 1.0 HH21 B:ARG31 4.0 43.3 1.0 CB B:LEU55 4.0 37.2 1.0 CG B:LEU55 4.0 41.9 1.0 O B:ILE53 4.1 29.6 1.0 HG2 B:ARG54 4.2 65.7 1.0 HH11 B:ARG31 4.2 47.2 1.0 CA B:LEU55 4.2 29.7 1.0 CD1 B:LEU55 4.3 34.6 1.0 HB3 B:ARG54 4.4 42.0 1.0 CB B:ARG54 4.5 35.0 1.0 O B:LEU55 4.7 41.5 1.0 HD11 B:LEU55 4.7 41.5 1.0 N B:ARG54 4.7 30.8 1.0 CZ B:PHE32 4.8 32.2 1.0 CG B:ARG54 4.8 54.8 1.0 C B:ILE53 4.8 40.8 1.0 HB3 B:LEU55 4.9 44.7 1.0 HA B:LEU55 5.0 35.6 1.0 C B:LEU55 5.0 49.3 1.0

Chlorine binding site 2 out of 2 in the Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of the GLUK3 Ligand Binding Domain Complex with Glutamate and Zinc: P2221 Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl264 b:0.6 occ:1.00 HH21 B:ARG24 2.9 81.6 1.0 HH22 B:ARG20 3.6 69.6 1.0 O B:HOH345 3.6 53.0 1.0 NH2 B:ARG24 3.6 68.0 1.0 HE B:ARG24 3.6 80.3 1.0 HH21 B:ARG20 3.7 69.6 1.0 NH2 B:ARG20 3.8 58.0 1.0 HD21 B:LEU26 4.0 51.6 1.0 HG B:SER22 4.1 58.9 1.0 HH22 B:ARG24 4.1 81.6 1.0 OG B:SER22 4.3 49.1 1.0 NE B:ARG24 4.3 66.9 1.0 CZ B:ARG24 4.4 73.6 1.0 HB2 B:SER22 4.7 53.5 1.0 HD22 B:LEU26 4.8 51.6 1.0 CD2 B:LEU26 4.8 43.0 1.0 CZ B:ARG20 4.9 56.0 1.0

J.Veran, J.Kumar, P.S.Pinheiro, A.Athane, M.L.Mayer, D.Perrais, C.Mulle. Zinc Potentiates GLUK3 Glutamate Receptor Function By Stabilizing the Ligand Binding Domain Dimer Interface. Neuron V. 76 565 2012.
ISSN: ISSN 0896-6273
PubMed: 23141068
DOI: 10.1016/J.NEURON.2012.08.027