Chlorine in PDB 3vk1: Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5

The structure of Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5, PDB code: 3vk1 was solved by J.M.Battad, D.A.K.Traore, M.Wilce, M.Byres, J.Rossjohn, R.J.Devenish, M.Prescott, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.62 / 2.20
Space group	P 42 2 2
Cell size a, b, c (Å), α, β, γ (°)	93.120, 93.120, 76.910, 90.00, 90.00, 90.00
R / Rfree (%)	20.5 / 24.2

The structure of Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5 also contains other interesting chemical elements:

Iodine

(I)

1 atom

The binding sites of Chlorine atom in the Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5 (pdb code 3vk1). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5, PDB code: 3vk1:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl227 b:68.0 occ:1.00 OG A:SER146 3.2 40.3 1.0 CB A:ARG197 3.9 46.4 1.0 SD A:MET163 4.0 51.0 1.0 O A:GLU144 4.0 37.7 1.0 CB A:SER146 4.0 36.8 1.0 CB A:LEU199 4.0 51.1 1.0 CE A:MET163 4.1 45.2 1.0 O A:ARG197 4.1 48.7 1.0 N A:LEU199 4.2 51.4 1.0 N A:SER146 4.3 39.2 1.0 C A:ARG197 4.3 49.6 1.0 CB A:TRP143 4.6 38.7 1.0 CA A:LYS198 4.6 52.3 1.0 C A:LYS198 4.7 51.8 1.0 CD1 A:QFG66 4.7 76.4 1.0 N A:LYS198 4.7 51.4 1.0 CD A:ARG197 4.7 48.6 1.0 OE1 A:GLU215 4.8 70.6 1.0 CA A:PRO145 4.8 39.6 1.0 CA A:ARG197 4.8 49.0 1.0 CA A:SER146 4.8 39.4 1.0 CA A:LEU199 4.8 52.0 1.0 C A:PRO145 4.8 38.9 1.0 CD1 A:LEU199 4.8 52.8 1.0 CG A:LEU199 4.8 53.1 1.0 O A:HOH231 4.9 42.9 1.0 CG A:ARG197 5.0 48.1 1.0

Chlorine binding site 2 out of 3 in the Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl228 b:31.3 occ:1.00 O A:HOH253 3.5 35.9 1.0 NZ A:LYS182 3.6 36.5 1.0 CE A:MET156 4.1 48.7 1.0 CE A:LYS182 4.2 42.3 1.0 SD A:MET156 4.2 48.2 1.0 O A:HOH291 4.5 66.2 1.0 CG2 A:THR180 4.6 36.1 1.0 OE1 A:GLU94 4.7 36.8 1.0

Chlorine binding site 3 out of 3 in the Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Green-Fluorescent Variant of the Non-Fluorescent Chromoprotein RTMS5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl229 b:45.3 occ:1.00 O A:HOH317 3.1 60.1 1.0 N A:GLY155 3.4 47.0 1.0 CG A:MET189 3.9 53.9 1.0 N A:GLY154 4.0 44.6 1.0 CA A:GLY155 4.1 49.9 1.0 N A:ARG153 4.1 42.6 1.0 CB A:ALA152 4.1 46.5 1.0 CA A:ARG153 4.2 42.5 1.0 C A:ARG153 4.2 43.2 1.0 CE A:MET189 4.2 52.0 1.0 C A:ALA152 4.3 43.9 1.0 O A:HOH314 4.4 49.5 1.0 C A:GLY154 4.4 47.1 1.0 CA A:GLY154 4.6 45.4 1.0 CB A:MET189 4.6 54.9 1.0 O A:ALA152 4.7 44.6 1.0 SD A:MET189 4.7 54.8 1.0 CA A:ALA152 4.8 44.4 1.0 O A:ARG153 4.9 42.0 1.0

J.M.Battad, D.A.Traore, E.Byres, J.Rossjohn, R.J.Devenish, S.Olsen, M.C.Wilce, M.Prescott. A Green Fluorescent Protein Containing A Qfg Tri-Peptide Chromophore: Optical Properties and X-Ray Crystal Structure. Plos One V. 7 47331 2012.
ISSN: ESSN 1932-6203
PubMed: 23071789
DOI: 10.1371/JOURNAL.PONE.0047331