Chlorine in PDB 3vlz: Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf

All present enzymatic activity of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf:
1.7.7.1;

The structure of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf, PDB code: 3vlz was solved by S.Nakano, M.Takahashi, A.Sakamoto, H.Morikawa, K.Katayanagi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.20 / 2.07
Space group	P 4 21 2
Cell size a, b, c (Å), α, β, γ (°)	132.889, 132.889, 77.646, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 28.2

The structure of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf also contains other interesting chemical elements:

Potassium	(K)	1 atom
Iron	(Fe)	5 atoms

The binding sites of Chlorine atom in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf (pdb code 3vlz). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf, PDB code: 3vlz:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl604 b:30.5 occ:1.00 O A:HOH1251 2.8 45.0 1.0 N A:MET175 3.0 26.3 1.0 O A:HOH1065 3.3 28.1 1.0 CA A:MET175 3.6 26.8 1.0 CD A:LYS91 3.9 24.0 1.0 NH1 A:ARG179 3.9 9.0 1.0 CG A:MET175 3.9 28.5 1.0 CE2 A:PHE96 4.0 18.7 1.0 CMA A:SRM601 4.0 24.1 1.0 C A:GLY174 4.1 26.4 1.0 CA A:GLY174 4.3 25.7 1.0 CB A:MET175 4.3 26.8 1.0 SD A:MET175 4.3 30.5 1.0 O A:HOH1712 4.3 39.8 1.0 O A:HOH1200 4.4 37.0 1.0 CD2 A:PHE96 4.5 21.1 1.0 NZ A:LYS91 4.6 22.3 1.0 CE A:LYS91 4.6 23.3 1.0 C A:MET175 4.8 26.4 1.0 O2 A:SO3606 4.8 27.4 1.0 CG A:LYS91 4.8 23.1 1.0 CB A:LYS91 4.9 23.4 1.0 CZ A:ARG179 4.9 15.1 1.0 CHA A:SRM601 5.0 17.3 1.0 CZ A:PHE96 5.0 20.6 1.0

Chlorine binding site 2 out of 2 in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - SO3 Full Complex From Tobacco Leaf within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl605 b:31.7 occ:1.00 O A:HOH1127 3.3 38.7 1.0 O A:HOH1018 3.3 27.7 1.0 N A:LYS100 3.5 25.7 1.0 N A:ARG99 3.6 23.4 1.0 CA A:GLY447 3.7 20.5 1.0 CB A:LYS100 3.9 26.1 1.0 N A:GLY447 4.0 20.2 1.0 O A:HIS97 4.1 20.3 1.0 CA A:ARG99 4.1 24.4 1.0 CB A:ARG99 4.1 24.5 1.0 C A:ARG98 4.2 22.3 1.0 C A:ARG99 4.3 24.9 1.0 CA A:LYS100 4.3 26.1 1.0 CA A:ARG98 4.4 22.2 1.0 O A:HOH1419 4.6 33.5 1.0 C A:GLY447 4.7 21.1 1.0 O A:HOH1698 4.7 51.6 1.0 O A:ARG98 4.9 21.8 1.0 N A:ASN101 5.0 26.1 1.0

S.Nakano, M.Takahashi, A.Sakamoto, H.Morikawa, K.Katayanagi. X-Ray Crystal Structure of A Mutant Assimilatory Nitrite Reductase That Shows Sulfite Reductase-Like Activity Chem.Biodivers. V. 9 1989 2012.
ISSN: ISSN 1612-1872
PubMed: 22976986
DOI: 10.1002/CBDV.201100442