Chlorine in PDB 3vm0: Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf

All present enzymatic activity of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf:
1.7.7.1;

The structure of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf, PDB code: 3vm0 was solved by S.Nakano, M.Takahashi, A.Sakamoto, H.Morikawa, K.Katayanagi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.76 / 1.70
Space group	P 4 21 2
Cell size a, b, c (Å), α, β, γ (°)	133.229, 133.229, 77.644, 90.00, 90.00, 90.00
R / Rfree (%)	16.3 / 18.6

The structure of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf also contains other interesting chemical elements:

Potassium	(K)	1 atom
Iron	(Fe)	5 atoms

The binding sites of Chlorine atom in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf (pdb code 3vm0). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf, PDB code: 3vm0:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl604 b:24.0 occ:1.00 O A:HOH1285 3.0 26.6 1.0 N A:MET175 3.1 13.2 1.0 O A:HOH1065 3.1 20.9 1.0 CA A:MET175 3.7 14.5 1.0 NH1 A:ARG179 3.8 12.9 1.0 CE2 A:PHE96 3.8 11.4 1.0 CD A:LYS91 3.9 16.8 1.0 CG A:MET175 4.0 17.6 1.0 O A:HOH1054 4.0 38.5 1.0 CMA A:SRM601 4.0 13.3 1.0 SD A:MET175 4.1 21.0 1.0 C A:GLY174 4.1 13.3 1.0 CA A:GLY174 4.2 13.0 1.0 CB A:MET175 4.4 14.9 1.0 CD2 A:PHE96 4.5 13.7 1.0 CZ A:ARG179 4.7 12.0 1.0 CZ A:PHE96 4.8 13.4 1.0 O A:HOH1751 4.8 38.2 1.0 NZ A:LYS91 4.8 22.1 1.0 CE A:LYS91 4.8 19.2 1.0 CG A:LYS91 4.8 14.4 1.0 O3D A:SRM601 4.9 22.7 1.0 CB A:LYS91 4.9 13.6 1.0 CHA A:SRM601 4.9 12.5 1.0 C A:MET175 4.9 14.2 1.0 O A:HOH1221 5.0 33.8 1.0

Chlorine binding site 2 out of 2 in the Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Assimilatory Nitrite Reductase (NII3) - N226K Mutant - NO2 Complex From Tobacco Leaf within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl556 b:22.4 occ:1.00 O A:HOH1133 3.0 26.7 1.0 O A:HOH1018 3.1 15.9 1.0 N A:LYS100 3.3 16.5 1.0 N A:ARG99 3.5 15.1 1.0 CA A:GLY447 3.8 13.8 1.0 CB A:LYS100 3.8 18.1 1.0 CA A:ARG99 4.1 15.2 1.0 CB A:ARG99 4.1 15.0 1.0 O A:HOH1417 4.1 45.1 1.0 N A:GLY447 4.1 13.6 1.0 C A:ARG98 4.2 14.4 1.0 CA A:LYS100 4.2 17.8 1.0 C A:ARG99 4.2 15.7 1.0 O A:HIS97 4.2 13.9 1.0 CA A:ARG98 4.5 14.4 1.0 O A:HOH1499 4.8 36.6 1.0 C A:GLY447 4.8 14.7 1.0 N A:ASN101 4.9 18.1 1.0 O A:ARG98 4.9 14.3 1.0 NH1 A:ARG98 5.0 13.6 1.0

S.Nakano, M.Takahashi, A.Sakamoto, H.Morikawa, K.Katayanagi. X-Ray Crystal Structure of A Mutant Assimilatory Nitrite Reductase That Shows Sulfite Reductase-Like Activity Chem.Biodivers. V. 9 1989 2012.
ISSN: ISSN 1612-1872
PubMed: 22976986
DOI: 10.1002/CBDV.201100442