Chlorine in PDB 3zfk: N-Terminal Truncated Nuclease Domain of Colicin E7

The structure of N-Terminal Truncated Nuclease Domain of Colicin E7, PDB code: 3zfk was solved by E.Toth, A.Czene, B.Gyurcsik, H.Otten, J.-C.N.Poulsen, S.Larsen, H.E.M.Christensen, K.Nagata, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.099 / 1.70
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	55.400, 55.400, 73.200, 90.00, 90.00, 120.00
R / Rfree (%)	20 / 23.43

The structure of N-Terminal Truncated Nuclease Domain of Colicin E7 also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Chlorine atom in the N-Terminal Truncated Nuclease Domain of Colicin E7 (pdb code 3zfk). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the N-Terminal Truncated Nuclease Domain of Colicin E7, PDB code: 3zfk:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the N-Terminal Truncated Nuclease Domain of Colicin E7


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of N-Terminal Truncated Nuclease Domain of Colicin E7 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl1133 b:46.7 occ:1.00 O A:HOH2011 2.3 42.3 1.0 O A:HOH2010 2.4 38.2 1.0 H A:GLY455 2.9 28.6 1.0 O A:HOH2085 3.0 39.5 1.0 HB A:THR454 3.2 20.3 1.0 HA2 A:GLY455 3.6 23.9 1.0 N A:GLY455 3.6 23.8 1.0 CA A:GLY455 4.1 20.0 1.0 O A:HOH2081 4.1 29.9 1.0 CB A:THR454 4.2 16.9 1.0 C A:THR454 4.6 21.1 1.0 HA A:THR454 4.6 22.3 1.0 HD3 A:ARG485 4.7 42.4 1.0 CA A:THR454 4.8 18.6 1.0 HG23 A:THR454 4.8 36.3 1.0 C A:GLY455 4.8 19.6 1.0 HG3 A:LYS456 4.8 45.2 1.0 HA3 A:GLY455 4.9 23.9 1.0 HG1 A:THR454 4.9 27.0 1.0 OG1 A:THR454 4.9 22.5 1.0 HG22 A:THR454 4.9 36.3 1.0 CG2 A:THR454 4.9 30.2 1.0

Chlorine binding site 2 out of 2 in the N-Terminal Truncated Nuclease Domain of Colicin E7


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of N-Terminal Truncated Nuclease Domain of Colicin E7 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl1133 b:50.6 occ:1.00 O B:HOH2010 2.2 38.4 1.0 O B:HOH2009 2.3 42.3 1.0 O B:HOH2077 2.8 39.3 1.0 H B:GLY455 2.9 29.8 1.0 HB B:THR454 3.3 22.1 1.0 HA2 B:GLY455 3.5 22.2 1.0 N B:GLY455 3.6 24.8 1.0 O B:HOH2074 4.0 30.1 1.0 CA B:GLY455 4.1 18.5 1.0 CB B:THR454 4.3 18.4 1.0 C B:THR454 4.7 19.9 1.0 HD3 B:ARG485 4.7 44.0 1.0 HA B:THR454 4.7 23.5 1.0 C B:GLY455 4.8 18.9 1.0 HG23 B:THR454 4.8 37.4 1.0 CA B:THR454 4.8 19.6 1.0 HA3 B:GLY455 4.9 22.2 1.0 CG2 B:THR454 5.0 31.1 1.0

A.Czene, E.Toth, E.Nemeth, H.Otten, J.-C.N.Poulsen, H.E.M.Christensen, L.Rulisek, K.Nagata, S.Larsen, B.Gyurcsik. Tracing the Reason of Inactivity of A Mutant Colicin E7 Metallonuclease: A Hint to An Alternative Catalytic Mechanism? To Be Published.