Chlorine in PDB 4e3u: Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline

All present enzymatic activity of Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline:
3.2.1.17;

The structure of Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline, PDB code: 4e3u was solved by J.J.Tanner, T.A.Pemberton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.50 / 1.50
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	77.995, 77.995, 37.740, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 21.6

The binding sites of Chlorine atom in the Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline (pdb code 4e3u). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline, PDB code: 4e3u:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl202 b:27.5 occ:1.00 O A:HOH361 2.9 32.4 1.0 N A:GLY26 3.0 11.5 1.0 OG A:SER24 3.2 17.2 1.0 CA A:GLY26 3.4 11.1 1.0 CB A:SER24 3.4 13.7 1.0 CA A:GLN121 3.7 21.4 1.0 CD1 A:ILE124 3.8 32.7 1.0 N A:LEU25 4.1 12.8 1.0 N A:GLN121 4.1 21.1 1.0 CB A:GLN121 4.1 27.4 1.0 C A:LEU25 4.2 13.0 1.0 CG1 A:ILE124 4.2 25.4 1.0 C A:SER24 4.3 12.7 1.0 O A:VAL120 4.4 16.8 1.0 CG A:GLN121 4.4 33.6 1.0 C A:VAL120 4.5 17.3 1.0 CA A:SER24 4.5 11.8 1.0 C A:GLY26 4.5 13.6 1.0 CA A:LEU25 4.5 10.4 1.0 CG2 A:VAL120 4.6 20.0 1.0 N A:ASN27 4.7 12.7 1.0 O A:SER24 4.8 13.3 1.0 CB A:LEU25 4.8 12.6 1.0 C A:GLN121 4.8 15.9 1.0 O A:HOH325 5.0 34.9 1.0

Chlorine binding site 2 out of 3 in the Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl203 b:18.4 occ:1.00 ND2 A:ASN113 3.3 13.9 1.0 CB A:ASN113 3.5 10.6 1.0 CA A:ALA110 3.9 11.7 1.0 CG A:ASN113 3.9 12.1 1.0 O A:VAL109 4.0 14.9 1.0 CG1 A:VAL109 4.2 19.0 1.0 N A:ALA110 4.2 12.5 1.0 C A:VAL109 4.3 12.3 1.0 CD A:ARG114 4.5 14.6 1.0 CB A:ALA110 4.5 15.6 1.0 CG A:ARG114 4.5 13.6 1.0 CA A:ASN113 4.9 13.0 1.0 C A:ALA110 4.9 13.0 1.0 O A:ALA110 4.9 13.1 1.0

Chlorine binding site 3 out of 3 in the Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of Hen Egg White Lysozyme Cryoprotected in Proline within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl204 b:35.1 occ:1.00 O A:HOH320 3.0 17.4 1.0 N A:THR69 3.1 13.6 1.0 O A:THR69 3.3 16.9 1.0 N A:ARG68 3.5 16.5 1.0 OG A:SER72 3.6 29.2 1.0 C A:THR69 3.6 16.0 1.0 C A:GLY67 3.6 24.0 1.0 N A:GLY67 3.6 14.8 1.0 CA A:GLY67 3.6 17.9 1.0 OD1 A:ASN65 3.7 23.1 1.0 CA A:THR69 3.8 14.2 1.0 O A:HOH322 4.0 24.2 1.0 CB A:THR69 4.1 14.9 1.0 C A:ARG68 4.1 18.4 1.0 OD1 A:ASP66 4.2 12.7 1.0 CA A:ARG68 4.2 15.1 1.0 O A:GLY67 4.2 24.5 1.0 N A:PRO70 4.3 20.8 1.0 O A:HOH321 4.6 15.3 1.0 CB A:SER72 4.7 27.9 1.0 C A:ASP66 4.7 12.2 1.0 OG1 A:THR69 4.7 13.1 1.0 N A:ASP66 4.7 10.8 1.0 CG A:ASN65 4.8 18.4 1.0 CA A:PRO70 4.9 23.1 1.0

T.A.Pemberton, B.R.Still, E.M.Christensen, H.Singh, D.Srivastava, J.J.Tanner. Proline: Mother Nature'S Cryoprotectant Applied to Protein Crystallography. Acta Crystallogr.,Sect.D V. 68 1010 2012.
ISSN: ISSN 0907-4449
PubMed: 22868767
DOI: 10.1107/S0907444912019580