Chlorine in PDB 4ene: Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex

The structure of Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex, PDB code: 4ene was solved by H.H.Lim, T.Shane, C.Miller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.87 / 2.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	232.260, 97.520, 173.960, 90.00, 132.87, 90.00
R / Rfree (%)	23.9 / 27.8

The binding sites of Chlorine atom in the Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex (pdb code 4ene). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 5 binding sites of Chlorine where determined in the Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex, PDB code: 4ene:
Jump to Chlorine binding site number: 1; 2; 3; 4; 5;

Chlorine binding site 1 out of 5 in the Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl501 b:42.2 occ:1.00 OH A:TYR445 2.9 49.9 1.0 OG A:SER107 3.1 36.8 1.0 N A:ILE356 3.2 43.9 1.0 CA A:GLY355 3.2 43.2 1.0 CB A:SER107 3.5 35.4 1.0 N A:PHE357 3.6 39.0 1.0 CD1 A:ILE109 3.7 43.3 1.0 C A:GLY355 3.7 41.0 1.0 CB A:PHE357 3.7 39.0 1.0 CZ A:TYR445 4.0 45.3 1.0 OE2 A:GLU148 4.0 47.2 1.0 CG A:GLU148 4.1 41.8 1.0 CA A:GLY149 4.1 45.1 1.0 N A:GLY149 4.2 42.1 1.0 CE2 A:TYR445 4.2 40.3 1.0 CG1 A:ILE109 4.3 41.1 1.0 CA A:PHE357 4.3 44.2 1.0 CA A:ILE356 4.3 46.1 1.0 CA A:SER107 4.4 40.5 1.0 CG2 A:ILE356 4.4 35.6 1.0 C A:ILE356 4.4 46.5 1.0 N A:GLY355 4.4 50.2 1.0 CD A:GLU148 4.6 48.0 1.0 CB A:ILE356 4.8 36.4 1.0 CG A:PHE357 4.9 44.2 1.0 O A:GLY355 4.9 38.8 1.0 CD1 A:PHE357 5.0 43.1 1.0

Chlorine binding site 2 out of 5 in the Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl502 b:60.6 occ:1.00 N A:SER107 3.0 38.4 1.0 CZ A:PHE348 3.7 38.0 1.0 CB A:SER107 3.7 35.4 1.0 C A:GLY106 3.8 43.7 1.0 CA A:SER107 3.8 40.5 1.0 CA A:GLY106 3.8 47.1 1.0 CG A:PRO110 3.9 38.7 1.0 N A:GLY108 3.9 41.9 1.0 CG2 A:ILE448 4.1 37.3 1.0 CD A:PRO110 4.1 33.2 1.0 O A:GLY105 4.1 50.0 1.0 CE1 A:PHE348 4.2 40.9 1.0 C A:SER107 4.4 37.6 1.0 CE2 A:PHE348 4.6 37.4 1.0 CB A:PRO110 4.6 32.7 1.0 CD1 A:ILE448 4.7 36.4 1.0 OG A:SER107 4.8 36.8 1.0 N A:GLY106 4.8 50.5 1.0 C A:GLY105 4.8 56.1 1.0 O A:GLY106 4.9 41.8 1.0

Chlorine binding site 3 out of 5 in the Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl503 b:43.9 occ:1.00 OH B:TYR445 2.6 50.9 1.0 OG B:SER107 3.0 40.5 1.0 N B:ILE356 3.3 38.5 1.0 CB B:SER107 3.4 37.2 1.0 CA B:GLY355 3.5 41.0 1.0 CZ B:TYR445 3.6 46.0 1.0 CD1 B:ILE109 3.6 40.7 1.0 N B:PHE357 3.7 33.8 1.0 CE2 B:TYR445 3.8 39.7 1.0 CB B:PHE357 3.9 40.5 1.0 C B:GLY355 3.9 38.2 1.0 CG2 B:ILE356 4.1 32.5 1.0 CG1 B:ILE109 4.2 39.7 1.0 CA B:ILE356 4.3 44.8 1.0 CA B:PHE357 4.4 44.1 1.0 CA B:SER107 4.4 41.6 1.0 OE2 B:GLU148 4.4 41.7 1.0 CG B:GLU148 4.4 41.3 1.0 CA B:GLY149 4.5 42.9 1.0 C B:ILE356 4.5 44.0 1.0 N B:GLY149 4.6 38.7 1.0 CB B:ILE356 4.7 33.6 1.0 CE1 B:TYR445 4.7 41.6 1.0 N B:GLY355 4.8 51.2 1.0 CG B:PHE357 4.9 44.9 1.0 CB B:ILE109 4.9 44.4 1.0 CD1 B:PHE357 4.9 43.6 1.0 CD B:GLU148 5.0 46.5 1.0 CE1 B:PHE348 5.0 38.6 1.0

Chlorine binding site 4 out of 5 in the Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl504 b:49.9 occ:1.00 N B:SER107 3.1 33.5 1.0 CG B:PRO110 3.5 35.3 1.0 N B:GLY108 3.6 36.5 1.0 CB B:SER107 3.6 37.2 1.0 CD B:PRO110 3.7 28.7 1.0 CA B:SER107 3.8 41.6 1.0 C B:GLY106 4.0 45.1 1.0 CG2 B:ILE448 4.0 36.8 1.0 CZ B:PHE348 4.1 37.8 1.0 O B:GLY105 4.1 45.7 1.0 O B:HOH609 4.1 42.7 1.0 CA B:GLY106 4.2 45.2 1.0 C B:SER107 4.2 36.3 1.0 CB B:PRO110 4.2 32.4 1.0 OG B:SER107 4.6 40.5 1.0 CE1 B:PHE348 4.6 38.6 1.0 CA B:GLY108 4.6 38.0 1.0 CD1 B:ILE448 4.8 35.7 1.0 O B:HOH631 4.9 39.9 1.0 N B:PRO110 4.9 30.8 1.0 C B:GLY105 5.0 55.6 1.0 CE2 B:PHE348 5.0 30.7 1.0

Chlorine binding site 5 out of 5 in the Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 5 of Structure of the N- and C-Terminal Trimmed Clc-EC1 Cl-/H+ Antiporter and Fab Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl501 b:65.6 occ:1.00 NH2 B:ARG205 3.0 64.9 1.0 NH2 A:ARG205 3.1 64.8 1.0 NH1 A:ARG205 3.1 57.7 1.0 NH1 B:ARG205 3.1 57.0 1.0 CZ B:ARG205 3.5 68.5 1.0 CZ A:ARG205 3.5 67.6 1.0 CA A:TYR210 3.8 57.5 1.0 CD1 A:TYR210 3.8 52.5 1.0 CA B:TYR210 4.1 51.1 1.0 CD1 B:TYR210 4.2 55.0 1.0 N A:TYR210 4.3 53.9 1.0 CB A:TYR210 4.4 42.4 1.0 O A:ARG209 4.4 61.8 1.0 N B:TYR210 4.5 49.7 1.0 CG A:TYR210 4.5 49.7 1.0 O B:ARG209 4.6 64.1 1.0 N A:THR211 4.6 60.0 1.0 C A:ARG209 4.6 55.8 1.0 CE1 A:TYR210 4.6 53.1 1.0 CB B:TYR210 4.7 47.1 1.0 C A:TYR210 4.7 57.7 1.0 C B:ARG209 4.7 54.7 1.0 NE A:ARG205 4.8 65.8 1.0 NE B:ARG205 4.8 67.2 1.0 CG B:TYR210 4.9 55.8 1.0

H.H.Lim, T.Shane, C.Miller. Intracellular Proton Access in A Cl(-)/H(+) Antiporter. Plos Biol. V. 10 01441 2012.
ISSN: ISSN 1544-9173
PubMed: 23239938
DOI: 10.1371/JOURNAL.PBIO.1001441