Chlorine in PDB 4gdm: Crystal Structure of E.Coli Menh

All present enzymatic activity of Crystal Structure of E.Coli Menh:
4.2.99.20;

The structure of Crystal Structure of E.Coli Menh, PDB code: 4gdm was solved by J.M.Johnston, E.N.Baker, Z.Guo, M.Jiang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.77 / 2.75
Space group	I 41 2 2
Cell size a, b, c (Å), α, β, γ (°)	116.664, 116.664, 476.949, 90.00, 90.00, 90.00
R / Rfree (%)	19.2 / 22.3

The binding sites of Chlorine atom in the Crystal Structure of E.Coli Menh (pdb code 4gdm). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 3 binding sites of Chlorine where determined in the Crystal Structure of E.Coli Menh, PDB code: 4gdm:
Jump to Chlorine binding site number: 1; 2; 3;

Chlorine binding site 1 out of 3 in the Crystal Structure of E.Coli Menh


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of E.Coli Menh within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:61.2 occ:1.00 OH A:TYR85 3.3 54.0 1.0 ND2 A:ASN233 3.3 44.8 1.0 OH A:TYR148 3.4 63.6 1.0 NH2 A:ARG168 3.6 39.9 1.0 CE1 A:TYR148 3.8 64.8 1.0 CZ A:TYR148 4.1 68.5 1.0 CB A:ASN233 4.1 42.3 1.0 CE2 A:TYR85 4.1 46.0 1.0 CZ A:TYR85 4.1 53.4 1.0 CE1 A:PHE153 4.2 63.5 1.0 CG A:ASN233 4.2 58.2 1.0 CD2 A:LEU164 4.3 61.3 1.0 CZ A:PHE153 4.4 62.5 1.0 NE A:ARG168 4.5 44.8 1.0 CZ A:ARG168 4.5 56.8 1.0 O A:HOH419 4.5 49.1 1.0 CD1 A:LEU164 4.9 61.0 1.0 O A:HOH408 5.0 45.8 1.0

Chlorine binding site 2 out of 3 in the Crystal Structure of E.Coli Menh


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of E.Coli Menh within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl303 b:48.4 occ:1.00 O B:HOH417 3.1 38.9 1.0 OH B:TYR148 3.3 59.2 1.0 OH B:TYR85 3.3 55.8 1.0 NH2 B:ARG168 3.4 66.0 1.0 ND2 B:ASN233 3.6 40.4 1.0 CE1 B:TYR148 3.8 54.8 1.0 CE2 B:TYR85 3.9 41.7 1.0 CZ B:TYR148 4.0 60.7 1.0 CZ B:TYR85 4.1 51.9 1.0 CD2 B:LEU164 4.1 53.5 1.0 CE1 B:PHE153 4.1 49.2 1.0 CZ B:ARG168 4.3 73.1 1.0 NE B:ARG168 4.4 57.1 1.0 CB B:ASN233 4.4 41.6 1.0 CZ B:PHE153 4.4 47.2 1.0 O B:HOH405 4.5 51.8 1.0 CG B:ASN233 4.5 56.5 1.0

Chlorine binding site 3 out of 3 in the Crystal Structure of E.Coli Menh


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of E.Coli Menh within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl302 b:49.6 occ:1.00 OH C:TYR148 3.1 49.4 1.0 NH2 C:ARG168 3.1 44.0 1.0 OH C:TYR85 3.3 45.0 1.0 O C:HOH404 3.4 43.6 1.0 ND2 C:ASN233 3.6 39.7 1.0 CE1 C:TYR148 3.8 49.5 1.0 CE2 C:TYR85 3.9 36.0 1.0 CZ C:TYR148 3.9 52.7 1.0 CZ C:ARG168 4.0 58.5 1.0 CZ C:TYR85 4.0 43.9 1.0 NE C:ARG168 4.0 44.2 1.0 O C:HOH406 4.1 54.3 1.0 CD2 C:LEU164 4.3 39.3 1.0 CE1 C:PHE153 4.3 51.4 1.0 CB C:ASN233 4.3 30.1 1.0 CG C:ASN233 4.4 51.7 1.0 CZ C:PHE153 4.5 50.6 1.0 O C:HOH405 4.9 38.5 1.0 CD1 C:LEU164 4.9 42.7 1.0

J.M.Johnston, M.Jiang, Z.Guo, E.N.Baker. Crystal Structures of E. Coli Native Menh and Two Active Site Mutants. Plos One V. 8 61325 2013.
ISSN: ESSN 1932-6203
PubMed: 23637813
DOI: 10.1371/JOURNAL.PONE.0061325