Atomistry » Chlorine » PDB 4gen-4glo » 4ghc
Atomistry »
  Chlorine »
    PDB 4gen-4glo »
      4ghc »

Chlorine in PDB 4ghc: Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution

Enzymatic activity of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution

All present enzymatic activity of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution:
1.13.11.15;

Protein crystallography data

The structure of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution, PDB code: 4ghc was solved by E.G.Kovaleva, J.D.Lipscomb, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 22.47 / 1.55
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 110.356, 151.736, 96.335, 90.00, 90.00, 90.00
R / Rfree (%) 11.7 / 14.8

Other elements in 4ghc:

The structure of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution also contains other interesting chemical elements:

Iron (Fe) 4 atoms
Calcium (Ca) 1 atom

Chlorine Binding Sites:

The binding sites of Chlorine atom in the Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution (pdb code 4ghc). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution, PDB code: 4ghc:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in 4ghc

Go back to Chlorine Binding Sites List in 4ghc
Chlorine binding site 1 out of 4 in the Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cl402

b:11.6
occ:1.00
 NH1 A:ARG243 3.2 10.4 1.0
NH1 A:ARG293 3.2 9.0 1.0
O A:HOH770 3.2 21.7 1.0
CE1 A:HIS248 3.3 9.2 1.0
NH2 A:ARG243 3.3 9.4 1.0
ND1 A:HIS248 3.4 9.3 1.0
CB A:ARG293 3.5 8.1 1.0
CG A:ARG293 3.6 8.3 1.0
CD A:ARG293 3.6 8.7 1.0
CZ A:ARG243 3.7 9.8 1.0
O A:ARG293 3.8 8.5 1.0
CA A:ARG293 3.9 7.9 1.0
CZ2 A:TRP304 4.0 10.8 1.0
CH2 A:TRP304 4.1 11.6 1.0
C A:ARG293 4.2 7.7 1.0
CZ A:ARG293 4.2 8.6 1.0
NE A:ARG293 4.4 8.6 1.0
NE2 A:HIS248 4.5 9.2 1.0
O A:HOH867 4.5 13.9 1.0
CE2 A:TRP304 4.6 10.3 1.0
CG A:HIS248 4.6 8.4 1.0
CZ3 A:TRP304 4.7 11.1 1.0
CE2 A:PHE257 4.8 8.9 1.0
CZ A:PHE257 4.8 9.0 1.0

Chlorine binding site 2 out of 4 in 4ghc

Go back to Chlorine Binding Sites List in 4ghc
Chlorine binding site 2 out of 4 in the Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cl403

b:10.3
occ:1.00
 NH1 B:ARG243 3.1 9.5 1.0
O B:HOH817 3.2 20.2 1.0
NH1 B:ARG293 3.2 9.4 1.0
NH2 B:ARG243 3.3 9.3 1.0
CE1 B:HIS248 3.3 8.2 1.0
ND1 B:HIS248 3.4 8.3 1.0
CB B:ARG293 3.5 8.7 1.0
CG B:ARG293 3.6 8.8 1.0
CD B:ARG293 3.6 9.0 1.0
CZ B:ARG243 3.7 8.9 1.0
O B:ARG293 3.8 8.8 1.0
CA B:ARG293 3.9 8.2 1.0
CZ2 B:TRP304 4.1 10.2 1.0
CH2 B:TRP304 4.1 10.7 1.0
C B:ARG293 4.2 8.2 1.0
CZ B:ARG293 4.2 9.2 1.0
NE B:ARG293 4.4 8.9 1.0
NE2 B:HIS248 4.5 8.2 1.0
O B:HOH906 4.5 11.6 1.0
CE2 B:TRP304 4.6 9.1 1.0
CG B:HIS248 4.7 7.8 1.0
CZ3 B:TRP304 4.7 11.2 1.0
CE2 B:PHE257 4.8 8.1 1.0
CZ B:PHE257 4.8 8.3 1.0
NE B:ARG243 5.0 8.6 1.0

Chlorine binding site 3 out of 4 in 4ghc

Go back to Chlorine Binding Sites List in 4ghc
Chlorine binding site 3 out of 4 in the Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cl402

b:12.2
occ:1.00
 NH1 C:ARG243 3.2 11.3 1.0
NH1 C:ARG293 3.2 11.3 1.0
CE1 C:HIS248 3.3 10.4 1.0
O C:HOH878 3.3 27.5 1.0
NH2 C:ARG243 3.3 10.6 1.0
ND1 C:HIS248 3.4 9.5 1.0
CB C:ARG293 3.6 10.1 1.0
CG C:ARG293 3.7 10.8 1.0
CD C:ARG293 3.7 10.7 1.0
CZ C:ARG243 3.7 10.8 1.0
O C:ARG293 3.9 10.7 1.0
CA C:ARG293 4.0 10.1 1.0
CZ2 C:TRP304 4.0 12.5 1.0
CH2 C:TRP304 4.1 13.1 1.0
CZ C:ARG293 4.2 10.8 1.0
C C:ARG293 4.3 9.9 1.0
NE C:ARG293 4.4 10.3 1.0
NE2 C:HIS248 4.4 9.7 1.0
O C:HOH831 4.5 13.4 1.0
CG C:HIS248 4.6 9.4 1.0
CE2 C:TRP304 4.7 11.7 1.0
CZ3 C:TRP304 4.7 13.6 1.0
CE2 C:PHE257 4.8 8.4 1.0
CZ C:PHE257 4.8 8.7 1.0

Chlorine binding site 4 out of 4 in 4ghc

Go back to Chlorine Binding Sites List in 4ghc
Chlorine binding site 4 out of 4 in the Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Structure of Y257F Variant of Homoprotocatechuate 2,3-Dioxygenase From B.Fuscum at 1.55 Ang Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cl402

b:11.1
occ:1.00
 NH1 D:ARG243 3.2 10.2 1.0
O D:HOH740 3.2 21.6 1.0
NH1 D:ARG293 3.3 9.3 1.0
CE1 D:HIS248 3.3 8.4 1.0
NH2 D:ARG243 3.3 9.7 1.0
ND1 D:HIS248 3.4 8.5 1.0
CB D:ARG293 3.6 8.2 1.0
CG D:ARG293 3.6 8.6 1.0
CD D:ARG293 3.7 8.5 1.0
CZ D:ARG243 3.7 9.7 1.0
O D:ARG293 3.8 8.4 1.0
CA D:ARG293 3.9 8.2 1.0
CZ2 D:TRP304 4.0 11.5 1.0
CH2 D:TRP304 4.0 11.5 1.0
C D:ARG293 4.2 8.3 1.0
CZ D:ARG293 4.3 9.2 1.0
NE D:ARG293 4.4 8.7 1.0
NE2 D:HIS248 4.4 8.3 1.0
O D:HOH823 4.5 13.2 1.0
CG D:HIS248 4.6 8.0 1.0
CE2 D:TRP304 4.6 10.4 1.0
CZ3 D:TRP304 4.7 11.8 1.0
CE2 D:PHE257 4.8 9.1 1.0
CZ D:PHE257 4.8 9.0 1.0

Reference:

E.G.Kovaleva, J.D.Lipscomb. Structural Basis For the Role of Tyrosine 257 of Homoprotocatechuate 2,3-Dioxygenase in Substrate and Oxygen Activation. Biochemistry V. 51 8755 2012.
ISSN: ISSN 0006-2960
PubMed: 23066739
DOI: 10.1021/BI301115C
Page generated: Sun Jul 21 14:38:06 2024

Last articles

Cl in 6COG
Cl in 6CST
Cl in 6CTW
Cl in 6CTZ
Cl in 6CTT
Cl in 6CSP
Cl in 6CU9
Cl in 6CTX
Cl in 6CTU
Cl in 6CTP
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy