Chlorine in PDB 4gx8: Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera

All present enzymatic activity of Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera:
2.7.7.7;

The structure of Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera, PDB code: 4gx8 was solved by A.Robinson, N.Horan, Z.-Q.Xu, N.E.Dixon, A.J.Oakley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.04 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	84.470, 56.630, 138.010, 90.00, 93.52, 90.00
R / Rfree (%)	21.3 / 24.6

The binding sites of Chlorine atom in the Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera (pdb code 4gx8). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 4 binding sites of Chlorine where determined in the Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera, PDB code: 4gx8:
Jump to Chlorine binding site number: 1; 2; 3; 4;

Chlorine binding site 1 out of 4 in the Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl401 b:30.5 occ:1.00 O A:HOH581 3.0 19.0 1.0 O A:HOH579 3.0 27.4 1.0 N A:ARG38 3.2 10.9 1.0 CG A:ARG38 3.6 18.4 0.5 O A:HOH584 3.6 18.8 1.0 CA A:THR37 3.7 5.9 1.0 CG A:MET46 3.8 12.8 1.0 O A:ALA36 3.8 13.0 1.0 CG A:ARG38 3.9 8.5 0.5 C A:THR37 4.0 9.7 1.0 CB A:ARG38 4.0 11.3 0.5 CB A:ARG38 4.1 13.2 0.5 CA A:MET46 4.1 11.8 1.0 CA A:ARG38 4.2 11.8 0.5 CA A:ARG38 4.2 13.1 0.5 CG2 A:THR37 4.2 14.5 1.0 CB A:MET46 4.4 15.3 1.0 O A:HOH646 4.5 27.6 1.0 O A:HOH611 4.5 24.4 1.0 NE A:ARG38 4.5 20.0 0.5 CD A:ARG38 4.5 18.7 0.5 CB A:THR37 4.6 7.1 1.0 O A:HOH602 4.6 22.8 1.0 CD A:ARG38 4.6 11.8 0.5 C A:ALA36 4.7 11.0 1.0 N A:THR37 4.7 8.7 1.0 N A:MET46 4.7 11.6 1.0 O A:SER42 4.8 9.4 1.0 O A:SER45 5.0 17.1 1.0

Chlorine binding site 2 out of 4 in the Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera within 5.0Å range: probe atom residue distance (Å) B Occ B:Cl401 b:32.0 occ:1.00 O B:HOH651 2.9 21.1 1.0 O B:HOH620 2.9 25.7 1.0 N B:ARG38 3.2 13.1 1.0 CG B:ARG38 3.6 21.7 1.0 CA B:THR37 3.8 9.6 1.0 CB B:ARG38 3.9 20.9 1.0 CG B:MET46 3.9 25.1 1.0 O B:ALA36 3.9 15.5 1.0 C B:THR37 4.0 11.5 1.0 CA B:MET46 4.0 12.5 1.0 CD B:ARG38 4.1 33.5 1.0 CA B:ARG38 4.1 15.0 1.0 CG2 B:THR37 4.2 14.9 1.0 CB B:MET46 4.4 17.6 1.0 NE B:ARG38 4.5 34.7 1.0 O B:HOH552 4.6 21.7 1.0 CB B:THR37 4.6 10.5 1.0 N B:MET46 4.6 14.6 1.0 O B:SER45 4.8 16.7 1.0 C B:ALA36 4.8 10.8 1.0 N B:THR37 4.8 8.5 1.0 O B:SER42 4.8 12.4 1.0 N B:SER47 4.9 18.2 1.0

Chlorine binding site 3 out of 4 in the Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 3 of Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl401 b:29.1 occ:1.00 O C:HOH563 2.9 25.4 1.0 O C:HOH569 3.0 23.6 1.0 N C:ARG38 3.2 12.3 1.0 CG C:ARG38 3.7 22.7 1.0 CA C:THR37 3.8 5.9 1.0 CG C:MET46 3.8 16.9 1.0 O C:HOH582 3.9 21.8 1.0 CB C:ARG38 3.9 19.5 1.0 O C:HOH763 3.9 36.1 1.0 O C:ALA36 3.9 14.1 1.0 C C:THR37 4.0 11.5 1.0 CA C:MET46 4.0 11.3 1.0 CA C:ARG38 4.1 14.8 1.0 CD C:ARG38 4.2 38.5 1.0 CG2 C:THR37 4.2 14.1 1.0 O C:HOH760 4.2 30.9 1.0 NE C:ARG38 4.3 44.7 1.0 CB C:MET46 4.4 12.8 1.0 CB C:THR37 4.6 8.5 1.0 N C:MET46 4.7 10.8 1.0 O C:HOH662 4.7 20.2 1.0 O C:SER45 4.7 17.3 1.0 N C:THR37 4.8 7.9 1.0 C C:ALA36 4.8 11.8 1.0 O C:SER42 4.8 8.6 1.0 N C:SER47 5.0 13.5 1.0 C C:SER45 5.0 14.0 1.0

Chlorine binding site 4 out of 4 in the Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 4 of Crystal Structure of A Dna Polymerase III Alpha-Epsilon Chimera within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl401 b:33.4 occ:1.00 O D:HOH654 3.0 21.4 1.0 O D:HOH657 3.0 21.5 1.0 N D:ARG38 3.2 10.1 1.0 O D:HOH708 3.6 20.9 1.0 NH1 D:ARG38 3.6 14.7 0.3 CZ D:ARG38 3.6 11.4 0.3 CB D:ARG38 3.6 15.3 0.3 NH2 D:ARG38 3.7 16.3 0.3 CA D:THR37 3.8 6.1 1.0 CG D:ARG38 3.8 15.4 0.7 CG D:MET46 3.9 16.2 1.0 O D:ALA36 3.9 11.8 1.0 CB D:ARG38 3.9 18.4 0.7 C D:THR37 4.0 8.8 1.0 CA D:ARG38 4.1 12.6 0.3 CA D:MET46 4.1 11.1 1.0 CA D:ARG38 4.1 12.6 0.7 NE D:ARG38 4.2 14.3 0.3 O D:HOH707 4.3 28.4 1.0 CG2 D:THR37 4.3 10.4 1.0 CD D:ARG38 4.3 21.9 0.7 CB D:MET46 4.4 14.4 1.0 O D:HOH516 4.5 22.8 1.0 NE D:ARG38 4.5 33.3 0.7 CG D:ARG38 4.6 17.4 0.3 CB D:THR37 4.6 5.5 1.0 C D:ALA36 4.7 12.3 1.0 N D:MET46 4.7 12.8 1.0 N D:THR37 4.8 7.8 1.0 CD D:ARG38 4.8 6.6 0.3 O D:SER42 4.9 8.9 1.0 O D:SER45 5.0 14.4 1.0

K.Ozawa, N.P.Horan, A.Robinson, H.Yagi, F.R.Hill, S.Jergic, Z.Q.Xu, K.V.Loscha, N.Li, M.Tehei, A.J.Oakley, G.Otting, T.Huber, N.E.Dixon. Proofreading Exonuclease on A Tether: the Complex Between the E. Coli Dna Polymerase III Subunits Alpha, {Varepsilon}, Theta and Beta Reveals A Highly Flexible Arrangement of the Proofreading Domain Nucleic Acids Res. V. 41 5354 2013.
ISSN: ISSN 0305-1048
PubMed: 23580545
DOI: 10.1093/NAR/GKT162