Chlorine in PDB 4hb3: Crystal Structure of CRM1(T539S)-Ran-RANBP1 with Weakly Bound Unmodeled Leptomycin B

The structure of Crystal Structure of CRM1(T539S)-Ran-RANBP1 with Weakly Bound Unmodeled Leptomycin B, PDB code: 4hb3 was solved by Q.Sun, Y.M.Chook, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.80
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	106.118, 106.118, 305.425, 90.00, 90.00, 90.00
R / Rfree (%)	24.1 / 27.6

The structure of Crystal Structure of CRM1(T539S)-Ran-RANBP1 with Weakly Bound Unmodeled Leptomycin B also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Chlorine atom in the Crystal Structure of CRM1(T539S)-Ran-RANBP1 with Weakly Bound Unmodeled Leptomycin B (pdb code 4hb3). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total only one binding site of Chlorine was determined in the Crystal Structure of CRM1(T539S)-Ran-RANBP1 with Weakly Bound Unmodeled Leptomycin B, PDB code: 4hb3:

Chlorine binding site 1 out of 1 in the Crystal Structure of CRM1(T539S)-Ran-RANBP1 with Weakly Bound Unmodeled Leptomycin B


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Crystal Structure of CRM1(T539S)-Ran-RANBP1 with Weakly Bound Unmodeled Leptomycin B within 5.0Å range: probe atom residue distance (Å) B Occ C:Cl1101 b:0.0 occ:1.00 O C:LYS605 3.0 79.9 1.0 CB C:TYR608 3.5 87.5 1.0 N C:TYR608 3.7 83.5 1.0 CE C:LYS567 3.7 87.0 1.0 NZ C:LYS567 3.8 85.7 1.0 C C:CYS606 4.0 80.0 1.0 C C:LYS605 4.1 80.0 1.0 CA C:CYS606 4.1 78.0 1.0 CG C:LYS607 4.2 81.9 1.0 N C:LYS607 4.2 79.8 1.0 CA C:TYR608 4.2 86.3 1.0 NZ C:LYS607 4.3 83.1 1.0 O C:CYS606 4.3 81.8 1.0 N C:CYS606 4.5 78.1 1.0 C C:LYS607 4.7 81.5 1.0 CG C:TYR608 4.8 89.6 1.0 CD C:LYS567 4.8 87.8 1.0 CA C:LYS607 4.8 80.8 1.0 CD2 C:HIS609 4.9 88.7 1.0 CE C:LYS607 5.0 82.5 1.0

Q.Sun, Y.P.Carrasco, Y.Hu, X.Guo, H.Mirzaei, J.Macmillan, Y.M.Chook. Nuclear Export Inhibition Through Covalent Conjugation and Hydrolysis of Leptomycin B By CRM1. Proc.Natl.Acad.Sci.Usa V. 110 1303 2013.
ISSN: ISSN 0027-8424
PubMed: 23297231
DOI: 10.1073/PNAS.1217203110