Chlorine in PDB 4ilt: Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E

The structure of Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E, PDB code: 4ilt was solved by C.M.Bianchetti, T.E.Takasuka, L.F.Bergeman, C.H.Harmann, B.G.Fox, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.56 / 2.55
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.192, 85.125, 70.264, 90.00, 95.53, 90.00
R / Rfree (%)	20.3 / 26.8

The structure of Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E also contains other interesting chemical elements:

Iron

(Fe)

4 atoms

The binding sites of Chlorine atom in the Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E (pdb code 4ilt). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E, PDB code: 4ilt:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl302 b:32.5 occ:1.00 N A:GLY194 3.2 33.0 1.0 ND2 A:ASN198 3.3 35.0 1.0 CA A:GLY194 4.0 29.5 1.0 C A:PRO193 4.1 30.7 1.0 CA A:PRO193 4.2 29.1 1.0 CZ A:PHE204 4.2 29.3 1.0 CG A:ASN198 4.3 29.4 1.0 CB A:ASN198 4.4 28.2 1.0 CD1 A:LEU209 4.4 32.9 1.0 O A:PHE192 4.6 31.5 1.0 CE1 A:PHE204 4.7 34.1 1.0 N A:GLU195 4.8 31.9 1.0 C A:GLY194 4.8 32.4 1.0 CD2 A:LEU209 4.9 29.1 1.0 CE2 A:PHE204 5.0 29.1 1.0

Chlorine binding site 2 out of 2 in the Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Structure of the Dioxygenase Domain of SACTE_2871, A Novel Dioxygenase Carbohydrate-Binding Protein Fusion From the Cellulolytic Bacterium Streptomyces Sp. Sirexaa-E within 5.0Å range: probe atom residue distance (Å) B Occ D:Cl302 b:31.3 occ:1.00 N D:GLY194 2.9 40.2 1.0 ND2 D:ASN198 3.3 32.1 1.0 CA D:GLY194 3.6 41.5 1.0 C D:PRO193 3.9 39.3 1.0 CA D:PRO193 4.0 38.0 1.0 CZ D:PHE204 4.2 30.7 1.0 CG D:ASN198 4.3 41.8 1.0 CD1 D:LEU209 4.3 33.0 1.0 CB D:ASN198 4.4 43.9 1.0 CE1 D:PHE204 4.5 36.9 1.0 N D:GLU195 4.5 40.7 1.0 C D:GLY194 4.5 42.5 1.0 O D:PHE192 4.5 37.2 1.0 CD2 D:LEU209 4.8 35.8 1.0 CB D:PRO193 5.0 33.7 1.0 O D:GLU195 5.0 46.0 1.0

C.M.Bianchetti, C.H.Harmann, T.E.Takasuka, G.L.Hura, K.Dyer, B.G.Fox. Fusion of Dioxygenase and Lignin-Binding Domains in A Novel Secreted Enzyme From Cellulolytic Streptomyces Sp. Sirexaa-E. J.Biol.Chem. V. 288 18574 2013.
ISSN: ISSN 0021-9258
PubMed: 23653358
DOI: 10.1074/JBC.M113.475848