Chlorine in PDB 4klj: Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min

All present enzymatic activity of Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min:
2.7.7.7;

The structure of Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min, PDB code: 4klj was solved by B.D.Freudenthal, W.A.Beard, D.D.Shock, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.99 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	51.000, 80.600, 55.500, 90.00, 107.90, 90.00
R / Rfree (%)	17.9 / 22.7

The structure of Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Sodium	(Na)	3 atoms

The binding sites of Chlorine atom in the Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min (pdb code 4klj). This binding sites where shown within 5.0 Angstroms radius around Chlorine atom.
In total 2 binding sites of Chlorine where determined in the Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min, PDB code: 4klj:
Jump to Chlorine binding site number: 1; 2;

Chlorine binding site 1 out of 2 in the Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 1 of Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl406 b:30.0 occ:1.00 N A:HIS34 3.3 26.8 1.0 CB A:HIS34 3.5 28.0 1.0 CB A:ALA32 3.5 31.8 1.0 C A:ALA32 3.6 29.8 1.0 CA A:ALA32 3.6 30.0 1.0 N A:LYS35 3.7 24.3 1.0 N A:ILE33 3.8 26.5 1.0 CA A:HIS34 3.8 28.2 1.0 ND1 A:HIS34 3.9 39.8 1.0 O A:ALA32 4.0 27.6 1.0 CG A:HIS34 4.1 28.0 1.0 C A:HIS34 4.2 24.8 1.0 CG A:LYS35 4.3 28.3 1.0 C A:ILE33 4.4 30.7 1.0 CA A:ILE33 4.6 30.8 1.0 CB A:LYS35 4.7 23.1 1.0 O6 D:DG1 4.7 28.6 1.0 O A:HOH607 4.7 41.4 1.0 CA A:LYS35 4.8 20.8 1.0

Chlorine binding site 2 out of 2 in the Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min


Mono view


Stereo pair view

A full contact list of Chlorine with other atoms in the Cl binding site number 2 of Dna Polymerase Beta Matched Product Complex with MG2+, 5 Min within 5.0Å range: probe atom residue distance (Å) B Occ A:Cl407 b:30.0 occ:1.00 NH2 A:ARG299 3.0 23.3 0.5 O A:HOH741 3.1 40.1 1.0 OG1 A:THR297 3.3 25.3 1.0 ND2 A:ASN294 3.4 24.2 1.0 NE A:ARG299 3.4 28.0 0.5 CZ A:ARG299 3.6 30.1 0.5 CG2 A:THR297 3.9 21.5 1.0 CZ A:ARG299 4.0 28.7 0.5 CB A:TYR296 4.0 20.2 1.0 OD1 A:ASN294 4.1 21.8 1.0 NH2 A:ARG299 4.1 27.2 0.5 NE A:ARG299 4.2 23.3 0.5 CG A:ASN294 4.2 19.1 1.0 CB A:THR297 4.2 23.8 1.0 CD2 A:TYR296 4.2 23.1 1.0 NH1 A:ARG299 4.4 25.4 0.5 CG A:TYR296 4.6 21.7 1.0 CD A:ARG299 4.7 26.5 0.5 CD A:ARG299 4.8 27.1 0.5 O T:HOH125 4.8 35.0 1.0 N A:THR297 4.8 22.5 1.0 O A:HOH568 4.8 24.4 1.0 NH1 A:ARG299 4.9 32.8 0.5 O A:HOH757 5.0 44.4 1.0

B.D.Freudenthal, W.A.Beard, D.D.Shock, S.H.Wilson. Observing A Dna Polymerase Choose Right From Wrong. Cell(Cambridge,Mass.) V. 154 157 2013.
ISSN: ISSN 0092-8674
PubMed: 23827680
DOI: 10.1016/J.CELL.2013.05.048